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Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect<jats:italic>T. gondii<\/jats:italic>RE gene in various human samples by<jats:italic>in house<\/jats:italic>and commercial real time polymerase chain reactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An<jats:italic>in house<\/jats:italic>and a commercial RT-PCR were applied to investigate the<jats:italic>T. gondii<\/jats:italic>RE gene in these samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The compatibility rate of the two tests was 94.7% (37\/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of<jats:italic>in house<\/jats:italic>RT-PCR test was 87.5 and 100%. When the<jats:italic>in house<\/jats:italic>RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both the commercial and<jats:italic>in house<\/jats:italic>RT-PCR tests can be used to investigate<jats:italic>T. gondii<\/jats:italic>RE gene in various clinical specimens with their high sensitivity and specificity. In house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12879-019-4666-z","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T11:04:07Z","timestamp":1575975847000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey"],"prefix":"10.1186","volume":"19","author":[{"given":"Mert","family":"D\u00f6\u015fkaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcsn\u00fc","family":"Pulluk\u00e7u","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet","family":"Karakavuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra Atalay","family":"\u015eahar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Sezai","family":"Ta\u015fbakan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meltem I\u015f\u0131kg\u00f6z","family":"Ta\u015fbakan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00fcmtaz","family":"Y\u0131lmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0363-9099","authenticated-orcid":false,"given":"Aysu De\u011firmenci","family":"D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan Y\u00fcksel","family":"G\u00fcr\u00fcz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,10]]},"reference":[{"key":"4666_CR1","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","volume":"363","author":"JG Montoya","year":"2004","unstructured":"Montoya JG, Liesenfeld O. 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