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This study aims to determine the role of household contacts of children diagnosed with pertussis in Casablanca Morocco.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>From November 2015 to October 2017, children suspected of whooping cough that consulted Ibn Rochd University hospital at Casablanca with their household contacts were enrolled in the study. Nasopharyngeal (NP) samples of the suspected children were analyzed by culture and RT-PCR. For the household contacts, NP and blood samples were collected and analyzed by RT-PCR and specific detection of pertussis toxin antibodies by ELISA, respectively.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>During the study period, the survey was carried out on 128 infants hospitalized for pertussis suspicion and their families (<jats:italic>N<\/jats:italic>\u2009=\u2009140). <jats:italic>B. pertussis<\/jats:italic> DNA was specifically detected in 73 (57%) samples, coexistence of <jats:italic>B. pertussis<\/jats:italic> and <jats:italic>B. parapertussis<\/jats:italic> DNA in 3 (2.3%) samples, coexistence of <jats:italic>B. pertussis<\/jats:italic> and <jats:italic>B. holmesii<\/jats:italic> DNA in 10 (7.81%) and only one (0.78%) sample was IS <jats:italic>481<\/jats:italic> RT-PCR positive without the possibility of determining the <jats:italic>Bordetella<\/jats:italic> species with the diagnostic tools used. Confirmations of Pertussis infection in household contacts by culture, RT- PCR and serology were 10, 46 and 39%, respectively.<\/jats:p>\n<jats:p><jats:italic>B. pertussis<\/jats:italic> DNA was confirmed in the infants as well in their mothers in 38% of the cases. Co detection of <jats:italic>B. pertussis<\/jats:italic> and <jats:italic>B. parapertussis<\/jats:italic> DNA in 2% and co-detection of <jats:italic>B. pertussis<\/jats:italic> and <jats:italic>B. holmesii<\/jats:italic> DNA in 4%. <jats:italic>B. holmesii<\/jats:italic> DNA alone was detected in 5 NP samples of index cases and their mothers.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The results of this study confirm that <jats:italic>B. pertussis<\/jats:italic> is still circulating in children and adults, and were likely a source of pertussis contamination in infants still not vaccinated. The use of RT-PCR specific for <jats:italic>B. pertussis<\/jats:italic> in the diagnosis of adults is less sensitive and should be associated with serologic tests to improve diagnosis of pertussis and contributes to preventing transmission of the disease in infants.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12879-019-4680-1","type":"journal-article","created":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T19:02:41Z","timestamp":1579028561000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco"],"prefix":"10.1186","volume":"20","author":[{"given":"Khalid","family":"Katfy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Idrissa","family":"Diawara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fakhredine","family":"Maaloum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siham","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Guiso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Fellah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bouchra","family":"Slaoui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid","family":"Zerouali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houria","family":"Belabbes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naima","family":"Elmdaghri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"key":"4680_CR1","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1378\/chest.13-2942","volume":"146","author":"JD Hartzell","year":"2014","unstructured":"Hartzell JD, Blaylock JM. 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The study was anonymous and all patients and household contacts, and their parents\/guardians for minors below 18\u2009years of age, were informed by their physicians about the nature of the study. Only patients or theirs parents\/guardians for minors who agreed to participate in this study signed the informed consent. The fact that samples will be stored for a certain period of time before testing was clearly indicated in the inform consent statement.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All authors, participants (The patients\/legal guardians) and partners agreed the publication of data presented in this manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"43"}}