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Despite this,\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      is not part of routine screening for Sexually Transmitted Infections (STIs) in many countries and the prevalence of infection and patterns of disease remain to be determined in many populations. Such data is of particular importance in light of the reported rise in antibiotic resistance in\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Urine and urethral swab samples were collected from the primary public sexual health clinic in Singapore and tested for\n                      <jats:italic>C. trachomatis (CT)<\/jats:italic>\n                      or\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      (NG) infection and for the presence of\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      . Antibiotic resistance in\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      strains detected was determined by screening for genomic mutations associated with macrolide and fluroquinolone resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We report the results of a study into\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      prevalence at the national sexual health clinic in Singapore.\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      was heavily associated with CT infection (8.1% of cases), but present in only of 2.4% in CT negative cases and not independently linked to NG infection. Furthermore, we found high rates of resistance mutations to both macrolides (25%) and fluoroquinolones (37.5%) with a majority of resistant strains being dual-resistant. Resistance mutations were only found in strains from patients with CT co-infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our results support targeted screening of CT positive patients for\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      as a cost-effective strategy to reduce the incidence of\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      in the absence of comprehensive routine screening. The high rate of dual resistance also highlights the need to ensure the availability of alternative antibiotics for the treatment of multi-drug resistant\n                      <jats:italic>M. genitalium<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12879-020-05019-1","type":"journal-article","created":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T12:33:42Z","timestamp":1588077222000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Mycoplasma genitalium in Singapore is associated with Chlamydia trachomatis infection and displays high macrolide and Fluoroquinolone resistance rates"],"prefix":"10.1186","volume":"20","author":[{"given":"Tim","family":"Hart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Ying","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Aminah bte","family":"Mansoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin T. 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