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As for healthy patients, reports on asymptomatic anaplasmosis resulting from such tick bites are rare.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Case presentation<\/jats:title>\n<jats:p>A 55-year-old female patient visited the hospital with a tick bite in the right infraclavicular region. The tick was suspected to have been on the patient for more than 10\u2009days. PCR and an indirect immunofluorescence assay (IFA) were performed to identify tick-borne infectious diseases. The blood sample collected at admission yielded a positive result in nested PCR targeting <jats:italic>Ehrlichia<\/jats:italic>- or <jats:italic>Anaplasma<\/jats:italic>-specific genes <jats:italic>groEL<\/jats:italic> and <jats:italic>ankA<\/jats:italic>. Subsequent sequencing confirmed the presence of <jats:italic>A<\/jats:italic>. <jats:italic>phagocytophilum<\/jats:italic>, and seroconversion was confirmed by the IFA involving an <jats:italic>A<\/jats:italic>. <jats:italic>phagocytophilum<\/jats:italic> antigen slide. PCR detected no <jats:italic>Rickettsia<\/jats:italic>-specific genes [outer membrane protein A (<jats:italic>ompA<\/jats:italic>) or surface cell antigen 1 (<jats:italic>sca1<\/jats:italic>)], but seroconversion of spotted fever group (SFG) rickettsiosis was confirmed by an IFA.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This study genetically and serologically confirmed an asymptomatic <jats:italic>A<\/jats:italic>. <jats:italic>phagocytophilum<\/jats:italic> infection. Although SFG rickettsiosis was not detected genetically, it was detected serologically. These findings indicate the possibility of an asymptomatic coinfection: anaplasmosis plus SFG rickettsiosis. It is, therefore, crucial for clinicians to be aware of potential asymptomatic anaplasmosis following a tick bite.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12879-020-05170-9","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T10:03:46Z","timestamp":1593511426000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Asymptomatic-anaplasmosis confirmation using genetic and serological tests and possible coinfection with spotted fever group Rickettsia: a case report"],"prefix":"10.1186","volume":"20","author":[{"given":"Jiyeon","family":"Yoo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Hoon","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choon-Mee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na Ra","family":"Yun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6373-0922","authenticated-orcid":false,"given":"Dong-Min","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,6,30]]},"reference":[{"issue":"1","key":"5170_CR1","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1186\/s12879-018-3321-4","volume":"18","author":"SW Kim","year":"2018","unstructured":"Kim SW, Kim CM, Kim DM, Yun NR. 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The adult human subject provided written informed consent to participate in the study. Animal experiment was performed in accordance with an approved animal use protocol from Chosun University Institutional Animal Care and Use Committee (CIACUC) under approval number CIACUC2017-S0026.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent was obtained from the patient for publication of this case report and any accompanying images.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"458"}}