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Toxoplasmosis is asymptomatic in healthy individuals, whereas it may lead to death in immune suppressed or deficient patients. A vaccine against <jats:italic>T. gondii<\/jats:italic> is required to prevent consequences of the infection. The aim of this study is to generate a multivalent recombinant protein vaccine against <jats:italic>T. gondii.<\/jats:italic><\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>49 previously discovered antigenic proteins of <jats:italic>T gondii<\/jats:italic> were evaluated by their expression level in <jats:italic>E. coli<\/jats:italic> and by comprehensive bioinformatics analyses to determine antigenic epitopes. Based on these analyses, six vaccine candidate proteins were selected to generate a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50\u2009V. Humoral and cellular immune responses were determined by flow cytometry and ELISA. Vaccinated mice were challenged with <jats:italic>T. gondii<\/jats:italic> Ankara strain tachyzoites.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>In mice vaccinated with hexavalent vaccine, strong total IgG (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001) and IgG2a (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) responses were induced compared to controls, the ratio of CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T lymphocytes secreting IFN-\u03b3 increased, and significantly higher extracellular IFN-\u03b3 secretion was achieved compared to the controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). The survival time of the vaccinated mice increased to 8.38\u2009\u00b1\u20092.13\u2009days which was significantly higher than controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Altogether, these results show that the hexavalent vaccine which is developed for the first time against <jats:italic>T. gondii<\/jats:italic> induced strong and balanced Th1 and Th2 immune responses as well as conferred significant protection against challenge with lethal toxoplasmosis in murine model.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12879-020-05220-2","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T08:04:00Z","timestamp":1594368240000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50\u2009V and determination of its protective efficacy against acute toxoplasmosis"],"prefix":"10.1186","volume":"20","author":[{"given":"Esra Atalay","family":"\u015eahar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan G\u00fcl\u00e7e","family":"\u0130z","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysu De\u011firmenci","family":"D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Kalantari-Dehaghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remziye","family":"Deveci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan Y\u00fcksel","family":"G\u00fcr\u00fcz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6868-008X","authenticated-orcid":false,"given":"Mert","family":"D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"5220_CR1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4161\/hv.22474","volume":"9","author":"R Verma","year":"2013","unstructured":"Verma R, Khanna P. 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