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Horizontal transferability were validated by transformation and conjugation analysis. Plasmid incompatibility typing and MLST analysis was also performed.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 117 isolates were found to be resistant to at least one of the aminoglycoside antibiotics. It was observed that 77 (65.8%) were positive for 16S rRNA methyltransferases. Among them thirty nine isolates were found to harbour only <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub>, whereas combination of genes were observed in three isolates (<jats:italic>bla<\/jats:italic><jats:sub>VEB<\/jats:sub>+ <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> in 2 isolates and <jats:italic>bla<\/jats:italic><jats:sub>PER<\/jats:sub>\u2009+\u2009<jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> in 1 isolate). <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>OXA-48<\/jats:sub> like genes were found in 23 and 9 isolates, respectively. All the resistance genes were conjugatively transferable, and incompatibility typing showed multiple 16S rRNA methyltransferase genes were originated from a single Inc. I1 group. MLST analysis detected 3 clones of <jats:italic>E.coli<\/jats:italic>ST4410, ST1341 and ST3906.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The present study identified emergence of three clones of <jats:italic>E.coli<\/jats:italic>, resistant to aminoglycoside -cephalosporin- carbapenem. This warrants immediate measures to trace their transmission dynamics in order to slow down their spread in clinical setting.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12879-020-05264-4","type":"journal-article","created":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T12:02:34Z","timestamp":1595678554000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expansion of acquired 16S rRNA methytransferases along with CTX-M-15, NDM and OXA-48 within three sequence types of Escherichia coli from northeast India"],"prefix":"10.1186","volume":"20","author":[{"given":"Jayalaxmi","family":"Wangkheimayum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohana","family":"Bhattacharjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaskar Jyoti","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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