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Notwithstanding, anti-malaria drug resistant biomarkers have not been characterized in blood donors with asymptomatic <jats:italic>P. falciparum<\/jats:italic> infection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A total of 771 blood donors were selected from five districts in the Greater Accra Region, Ghana. Each donor sample was screened with malaria rapid diagnostic test (RDT) kit and parasitaemia quantified microscopically. Dried blood spots from malaria positive samples were genotyped for <jats:italic>P. falciparum<\/jats:italic> chloroquine resistance transporter (<jats:italic>Pfcrt<\/jats:italic>), <jats:italic>P. falciparum<\/jats:italic> multi-drug resistance (<jats:italic>Pfmdr1<\/jats:italic>), <jats:italic>P. falciparum<\/jats:italic> dihydropteroate-synthetase (<jats:italic>Pfdhps<\/jats:italic>), <jats:italic>P. falciparum<\/jats:italic> dihydrofolate-reductase (<jats:italic>Pfdhfr<\/jats:italic>) and <jats:italic>Kelch 13<\/jats:italic> propeller domain on chromosome 13 (<jats:italic>Kelch 13<\/jats:italic>) genes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Of the 771 blood donors, 91 (11.8%) were positive by RDT. Analysis of sequence reads indicated successful genotyping of <jats:italic>Pfcrt<\/jats:italic>, <jats:italic>Pfmdr1<\/jats:italic>, <jats:italic>Pfdhfr<\/jats:italic>, <jats:italic>Pfdhps<\/jats:italic> and <jats:italic>Kelch 13<\/jats:italic> genes in 84.6, 81.3, 86.8, 86.9 and 92.3% of the isolates respectively. Overall, 21 different mutant haplotypes were identified in 69 isolates (75.8%). In <jats:italic>Pfcrt<\/jats:italic>, CVIET haplotype was observed in 11.6% samples while in <jats:italic>Pfmdr1,<\/jats:italic> triple mutation (resulting in YFN haplotype) was detected in 8.1% of isolates. In <jats:italic>Pfdhfr<\/jats:italic> gene, triple mutation resulting in IRNI haplotype and in <jats:italic>Pfdhps<\/jats:italic> gene, quintuple mutation resulting in AGESS haplotype was identified in 17.7% parasite isolates. Finally, five non-synonymous <jats:italic>Kelch 13<\/jats:italic> alleles were detected; C580Y (3.6%), P615L (4.8%), A578S (4.8%), I543V (2.4%) and A676S (1.2%) were detected.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Results obtained in this study indicated various frequencies of mutant alleles in <jats:italic>Pfcrt<\/jats:italic>, <jats:italic>Pfmdr1<\/jats:italic>, <jats:italic>Pfdhfr<\/jats:italic>, <jats:italic>Pfdhps<\/jats:italic> and <jats:italic>Kelch 13<\/jats:italic> genes from <jats:italic>P. falciparum<\/jats:italic> infected blood donors. These alleles could reduce the efficacy of standard malaria treatment in transfusion-transmitted malaria cases. Incorporating malaria screening into donor screening protocol to defer infected donors is therefore recommended.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12879-020-05266-2","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T12:03:38Z","timestamp":1595419418000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of putative drug resistant biomarkers in Plasmodium falciparum isolated from Ghanaian blood donors"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6489-167X","authenticated-orcid":false,"given":"Enoch","family":"Aninagyei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwabena Obeng","family":"Duedu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanko","family":"Rufai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Comfort Dede","family":"Tetteh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaretta Gloria","family":"Chandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Ampomah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond Omane","family":"Acheampong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,22]]},"reference":[{"key":"5266_CR1","first-page":"827","volume":"79","author":"LJ Bruce-Chwatt","year":"1982","unstructured":"Bruce-Chwatt LJ. 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