{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:52:27Z","timestamp":1740135147607,"version":"3.37.3"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Affiliated Hospital of Zunyi Medical University","award":["201808"],"award-info":[{"award-number":["201808"]}]},{"name":"Honghuagang science and Technology Department","award":["201811"],"award-info":[{"award-number":["201811"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become a public health emergency of international concern. SARS-CoV-2 RNA detection is the diagnostic criterion for coronavirus disease 2019 (COVID-19). Nevertheless, RNA detection has many limitations, such as being time-consuming and cost-prohibitive, and it must be performed in specialized laboratories. Virus antibody detection is a routine method for screening for multiple viruses, but data about SARS-CoV-2 antibody detection are limited.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Method<\/jats:title>\n<jats:p>Throat swabs and blood were collected from 67 suspected SARS-CoV-2 infection patients at the Affiliated Hospital of Zunyi Medical University and Zunyi Fourth People\u2019s Hospital isolated observation departments. Throat swab samples were subjected to SARS-CoV-2 RNA detection by real-time PCR. Blood was used subjected to SARS-CoV-2 IgG\/IgM detection by an enzyme-linked immunosorbent assay (ELISA) and gold immunochromatography assay (GICA). Blood underwent C-reactive protein detection by immunoturbidimetry, and white blood cells, neutrophil percentages and lymphocyte percentages were counted and calculated, respectively. Clinical symptoms, age and lifestyle habits (smoking and drinking) in all patients were recorded. Data were analysed using SPSS version 19. The results were confirmed by T and \u03c7<jats:sup>2<\/jats:sup> tests; correlations with detection results were analysed by kappa coefficients. Odds ratio (OR) and corrected OR values were analysed by logistic regression. <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 was considered statistically significant.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Of the 67 patients included in this study, 26 were SARS-CoV-2 RNA-positive. GICA IgM sensitivity was 50.9% (13\/26), and specificity was 90.2% (37\/41). ELISA IgM sensitivity was 76.9% (20\/26), and specificity was 90.2% (37\/41). ELISA IgG sensitivity was 76.9% (20\/26), and specificity was 95.1% (39\/41). The kappa coefficients between RNA detection and ELISA IgG, ELISA IgM, and GICA IgM results were 0.741 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), 0.681 (<jats:italic>P\u2009&lt;<\/jats:italic>\u00a00.01) and 0.430 (<jats:italic>P\u2009&lt;<\/jats:italic>\u00a00.01), respectively.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Among the candidate blood indicators, serum IgG and IgM detected by ELISA had the best consistency and validity when compared with standard RNA detection; these indicators can be used as potential preliminary screening tools to identify those who should undergo nucleic acid detection in laboratories without RNA detection abilities or as a supplement to RNA detection.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12879-020-05489-3","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T12:03:58Z","timestamp":1603195438000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative analysis of the main haematological indexes and RNA detection for the diagnosis of SARS-CoV-2 infection"],"prefix":"10.1186","volume":"20","author":[{"given":"Jialin","family":"Xiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuyi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiusheng","family":"Ran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhimin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7592-189X","authenticated-orcid":false,"given":"Daimin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanzhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,10,20]]},"reference":[{"issue":"10223","key":"5489_CR1","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/S0140-6736(20)30183-5","volume":"395","author":"C Huang","year":"2020","unstructured":"Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China [J]. Lancet. 2020;395(10223):497\u2013506.","journal-title":"Lancet"},{"issue":"10223","key":"5489_CR2","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/S0140-6736(20)30185-9","volume":"395","author":"C Wang","year":"2020","unstructured":"Wang C, Horby P, Hayden FG, et al. A novel coronavirus outbreak of global health concern [J]. Lancet. 2020;395(10223):470\u20133.","journal-title":"Lancet"},{"issue":"8","key":"5489_CR3","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1056\/NEJMoa2001017","volume":"382","author":"N Zhu","year":"2020","unstructured":"Zhu N, Zhang D, Wang W, et al. A novel coronavirus from patients with pneumonia in China, 2019[J]. N Engl J Med. 2020;382(8):727\u201333.","journal-title":"N Engl J Med"},{"key":"5489_CR4","doi-asserted-by":"crossref","unstructured":"Li Q, Guan X, Wu P, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus\u2013infected pneumonia [J]. N Engl J Med. 2020;382(13):1199\u20131207.","DOI":"10.1056\/NEJMoa2001316"},{"key":"5489_CR5","volume-title":"WHO: A novel coronavirus infection pneumonia outbreak constitutes an international public health emergency","author":"National Health Committee of the people's Republic of China","year":"2020","unstructured":"National Health Committee of the people's Republic of China. WHO: A novel coronavirus infection pneumonia outbreak constitutes an international public health emergency. 2020. http:\/\/www.gov.cn\/xinwen\/2020-01\/30\/content_5473224.htm."},{"key":"5489_CR6","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.ijid.2020.01.009","volume":"91","author":"DS Hui","year":"2020","unstructured":"Hui DS, I Azhar E, Madani T A, et al. The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health\u2014the latest 2019 novel coronavirus outbreak in Wuhan, China [J]. Int J Infect Dis. 2020;91:264\u20136.","journal-title":"Int J Infect Dis"},{"issue":"10226","key":"5489_CR7","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/S0140-6736(20)30360-3","volume":"395","author":"H Chen","year":"2020","unstructured":"Chen H, Guo J, Wang C, et al. Clinical characteristics and intrauterine vertical transmission potential of COVID-19 infection in nine pregnant women: a retrospective review of medical records [J]. Lancet. 2020;395(10226):809\u201315.","journal-title":"Lancet"},{"key":"5489_CR8","doi-asserted-by":"crossref","unstructured":"Dai W, Zhang H, Yu J, et al. CT imaging and differential diagnosis of COVID-19[J]. Can Assoc Radiol J. 2020;71(2):195\u2013200.","DOI":"10.1177\/0846537120913033"},{"key":"5489_CR9","doi-asserted-by":"crossref","unstructured":"Ai T, Yang Z, Hou H, et al. Correlation of Chest CT and RT-PCR Testing in Coronavirus Disease 2019 (COVID-19) in China: A report of 1014 cases [J]. Radiology. 2020;296(2):E32\u2013E40.","DOI":"10.1148\/radiol.2020200642"},{"key":"5489_CR10","doi-asserted-by":"crossref","unstructured":"Bai Y, Yao L, Wei T, et al. Presumed asymptomatic carrier transmission of COVID-19[J]. JAMA. 2020;323(14):1406\u20137.","DOI":"10.1001\/jama.2020.2565"},{"key":"5489_CR11","volume-title":"Novel coronavirus pneumonia 2019 prevention and control program (Fifth Edition)","author":"National Health Committee of the people's Republic of China","year":"2020","unstructured":"National Health Committee of the people's Republic of China. Novel coronavirus pneumonia 2019 prevention and control program (Fifth Edition). 2020. http:\/\/www.gov.cn\/zhengce\/zhengceku\/2020-02\/22\/content_5482010.htm."},{"issue":"2","key":"5489_CR12","doi-asserted-by":"publisher","first-page":"165","DOI":"10.4142\/jvs.2010.11.2.165","volume":"11","author":"HK Lee","year":"2010","unstructured":"Lee HK, Lee BH, Seok SH, et al. Production of specific antibodies against SARScoronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. J Vet Sci. 2010;11(2):165\u20137.","journal-title":"J Vet Sci"},{"issue":"3","key":"5489_CR13","first-page":"36","volume":"29","author":"ZYZX Wan","year":"2003","unstructured":"Wan ZYZX. Yan XG IFA in testing specific antibody of SARS coronavirus. South China J Prev Med. 2003;29(3):36\u20137.","journal-title":"South China J Prev Med"},{"issue":"1","key":"5489_CR14","first-page":"4","volume":"7","author":"YH Jin","year":"2020","unstructured":"Jin YH, Cai L, Cheng ZS, et al. A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)[J]. Mil Med Res. 2020;7(1):4.","journal-title":"Mil Med Res"},{"key":"5489_CR15","volume-title":"Infection prevention and control during health care when novel coronavirus (nCoV) infection is suspected: interim guidance [S]","author":"WHO","year":"2020","unstructured":"WHO. Infection prevention and control during health care when novel coronavirus (nCoV) infection is suspected: interim guidance [S]. Geneva: World Health Organization; 2020."},{"key":"5489_CR16","doi-asserted-by":"crossref","unstructured":"Guan W, Ni Z, Hu Y, et al. Clinical characteristics of coronavirus disease 2019 in China [J]. N Engl J Med. 2020;382(18):1708\u201320.","DOI":"10.1056\/NEJMoa2002032"},{"key":"5489_CR17","doi-asserted-by":"crossref","unstructured":"Li Z, Yi Y, Luo X, et al. Development and clinical application of A rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis [J]. J Med Virol. 2020.","DOI":"10.1002\/jmv.25727"},{"key":"5489_CR18","doi-asserted-by":"crossref","unstructured":"Jia X, Zhang P, Tian Y, et al. Clinical significance of IgM and IgG test for diagnosis of highly suspected COVID-19 infection [J]. medRxiv. 2020.","DOI":"10.1101\/2020.02.28.20029025"},{"key":"5489_CR19","doi-asserted-by":"crossref","unstructured":"Fang Y, Zhang H, Xie J, et al. Sensitivity of chest CT for COVID-19: comparison to RT-PCR [J]. Radiology. 2020;296(2):E115\u201317.","DOI":"10.1148\/radiol.2020200432"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-020-05489-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12879-020-05489-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-020-05489-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T23:44:55Z","timestamp":1634687095000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/s12879-020-05489-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,20]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["5489"],"URL":"https:\/\/doi.org\/10.1186\/s12879-020-05489-3","relation":{},"ISSN":["1471-2334"],"issn-type":[{"type":"electronic","value":"1471-2334"}],"subject":[],"published":{"date-parts":[[2020,10,20]]},"assertion":[{"value":"29 April 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 October 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The present study was approved by the Affiliated Hospital of Zunyi Medical University Ethics Committee under approval number was KLL-2020-008. Written informed consent was obtained from all the patients or their legal guardians\/representatives.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"779"}}