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We investigate the variation of malaria cases, parasite density and the multiplicity of\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      infection throughout the year in Brazzaville.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From May 2015 to May 2016, suspected patients with uncomplicated malaria were enrolled at the H\u00f4pital de Mfilou, CSI \u00ab Maman Mboual\u00e9\u00bb, and the Laboratoire National de Sant\u00e9 Publique. For each patient, thick blood was examined and parasite density was calculated. After DNA isolation, MSP1 and MSP2 genes were genotyped.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 416, 259 and 131 patients with suspected malaria were enrolled at the CSI \u00abMaman Mboual\u00e9\u00bb, H\u00f4pital de Mfilou and the Laboratoire National de Sant\u00e9 Publique respectively. Proportion of malaria cases and geometric mean parasite density were higher at the CSI \u00abMaman Mboual\u00e9\u00bb compared to over sites\n                      <jats:italic>(P-value<\/jats:italic>\n                      &lt;0.001). However the multiplicity of infection was higher at the H\u00f4pital de Mfilou (\n                      <jats:italic>P-value<\/jats:italic>\n                      &lt;0.001).\n                    <\/jats:p>\n                    <jats:p>\n                      At the Laboratoire National de Sant\u00e9 Publique, malaria cases and multiplicity of infection were not influenced by different seasons. However, variation of the mean parasite density was statistically significant (\n                      <jats:italic>P-value<\/jats:italic>\n                      &lt;0.01).\n                    <\/jats:p>\n                    <jats:p>Higher proportions of malaria cases were found at the end of main rainy season either the beginning of the main dry season at the H\u00f4pital de Mfilou and the CSI \u00abMaman Mboual\u00e9\u00bb; while, lowest proportions were observed in September and January and in September and March respectively. Higher mean parasite densities were found at the end of rainy seasons with persistence at the beginning of dry seasons. The lowest mean parasite densities were found during dry seasons, with persistence at the beginning of rainy seasons. Fluctuation of the multiplicity of infection throughout the year was observed without significance between seasons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The current study suggests that malaria transmission is still variable between the north and south parts of Brazzaville. Seasonal fluctuations of malaria cases and mean parasite densities were observed with some extension to different seasons. Thus, both meteorological and entomological studies are needed to update the season\u2019s periods as well as malaria transmission intensity in Brazzaville.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12879-020-4913-3","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T09:03:08Z","timestamp":1583312588000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Variation of prevalence of malaria, parasite density and the multiplicity of Plasmodium falciparum infection throughout the year at three different health centers in Brazzaville, Republic of Congo"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5611-4328","authenticated-orcid":false,"given":"Pembe Issamou","family":"Mayengue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezi","family":"Kouhounina Batsimba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roch Fabien","family":"Niama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyna","family":"Ibara Ottia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alida","family":"Malonga-Massanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gr\u00e2ce Petula Urielle","family":"Fila-Fila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Ahombo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Charles","family":"Kobawila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri Joseph","family":"Parra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"key":"4913_CR1","volume-title":"World malaria Report","author":"World Health Organization","year":"2018","unstructured":"World Health Organization. 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Before the recruitment, the project objectives, methodology and expected results have been explained to patients and\/or theirs parents\/guardians. Written and signed informed consent was obtained from all study participants or their parents or guardians.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"190"}}