{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T10:09:00Z","timestamp":1777975740537,"version":"3.51.4"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,2,27]],"date-time":"2021-02-27T00:00:00Z","timestamp":1614384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,27]],"date-time":"2021-02-27T00:00:00Z","timestamp":1614384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007279","name":"Shanghai Municipal Health Bureau","doi-asserted-by":"publisher","award":["shslczdzk06902"],"award-info":[{"award-number":["shslczdzk06902"]}],"id":[{"id":"10.13039\/501100007279","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Respiratory tract infections (RTIs) are the common diseases in children and the routine detection methods frequently fail to identify the infectious pathogens especially for viruses. The Filmarray respiratory panel (FARP) can reliably and rapidly identify viruses and bacteria pathogens. This study is to evaluate the performance and clinical significance of FARP in children.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Children diagnosed with RTIs in pediatric intensive care unit (PICU) were enrolled in this study. Nasopharyngeal secretion (NPS) samples of these children were collected and the FARP assay for 17 pathogens and routine microbiological methods were performed. Clinical data of all patients was also collected and evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 90 children were enrolled into this study and 58 patients (64.4%) were positive for 13 pathogens by FARP, with 18 being detected positive with multiple-virus (31.3%, 18\/58). Human rhinovirus\/enterovirus (21.0%%, 17\/58) were the predominant pathogen, followed by adenovirus (18.5%). Higher proportions of various pathogens were identified in the infant and toddler (0\u20132\u2009years) groups with human rhinovirus\/enterovirus being mostly virus. Adenovirus were common in the group aged 3\u20135\u2009years, but only three pathogens including\n                      <jats:italic>M.pneumoniae<\/jats:italic>\n                      , respiratory syncytial virus, and adenovirus were also found in age group (6\u201314\u2009years). Among 58 FARP positive patients, significant differences were found in antibiotic prescription and use of glucocorticoid between the single-organism-positive group and the multi-organism-positive group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u20090.05). Furthermore, there was significant difference in use of anti-virus and usage of glucocorticoid between severe respiratory infections group and non severe respiratory infections group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrated that FARP can provide the rapid detection of respiratory virus and atypical bacteria for children, especially with severe respiratory tract infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12879-021-05900-7","type":"journal-article","created":{"date-parts":[[2021,2,27]],"date-time":"2021-02-27T06:02:53Z","timestamp":1614405773000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The clinical application of Filmarray respiratory panel in children especially with severe respiratory tract infections"],"prefix":"10.1186","volume":"21","author":[{"given":"Fen","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0886-9531","authenticated-orcid":false,"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,2,27]]},"reference":[{"issue":"2","key":"5900_CR1","doi-asserted-by":"publisher","DOI":"10.2196\/publichealth.9307","volume":"4","author":"S de Lusignan","year":"2018","unstructured":"de Lusignan S, Correa A, Pebody R, Yonova I, Smith G, Byford R, et al. Incidence of lower respiratory tract infections and atopic conditions in boys and young male adults: Royal College of general practitioners research and surveillance Centre annual report 2015-2016. JMIR Public Health Surveill. 2018;4(2):e49.","journal-title":"JMIR Public Health Surveill"},{"issue":"10","key":"5900_CR2","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1097\/INF.0000000000000800","volume":"34","author":"JM Uitti","year":"2015","unstructured":"Uitti JM, Tahtinen PA, Laine MK, Huovinen P, Ruuskanen O, Ruohola A. Role of nasopharyngeal Bacteria and respiratory viruses in acute symptoms of young children. Pediatr Infect Dis J. 2015;34(10):1056\u201362.","journal-title":"Pediatr Infect Dis J"},{"issue":"9963","key":"5900_CR3","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/S0140-6736(14)61682-2","volume":"385","author":"GBD Mortality","year":"2015","unstructured":"Mortality GBD. Causes of death C. global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the global burden of disease study 2013. Lancet. 2015;385(9963):117\u201371.","journal-title":"Lancet."},{"key":"5900_CR4","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.jcv.2018.07.003","volume":"106","author":"ML Bouzas","year":"2018","unstructured":"Bouzas ML, Oliveira JR, Queiroz A, Fukutani KF, Barral A, Rector A, et al. Diagnostic accuracy of digital RNA quantification versus real-time PCR for the detection of respiratory syncytial virus in nasopharyngeal aspirates from children with acute respiratory infection. 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Yonsei Med J. 2010;51(5):761\u20137.","journal-title":"Yonsei Med J"},{"issue":"10","key":"5900_CR7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026047","volume":"6","author":"MA Poritz","year":"2011","unstructured":"Poritz MA, Blaschke AJ, Byington CL, Meyers L, Nilsson K, Jones DE, et al. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS One. 2011;6(10):e26047.","journal-title":"PLoS One"},{"issue":"12","key":"5900_CR8","doi-asserted-by":"publisher","first-page":"3784","DOI":"10.1128\/JCM.01516-15","volume":"53","author":"N Azadeh","year":"2015","unstructured":"Azadeh N, Sakata KK, Brighton AM, Vikram HR, Grys TE. FilmArray respiratory panel assay: comparison of nasopharyngeal swabs and Bronchoalveolar lavage samples. 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Written informed consent was obtained from the patients\u2019 guardians on behalf of the children enrolled in this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"230"}}