{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:22:41Z","timestamp":1790716961017,"version":"4.1.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T00:00:00Z","timestamp":1623456000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T00:00:00Z","timestamp":1623456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram and biofilm formation among pregnant women with fever, women with a history of spontaneous abortion, women with a history of fetal loss, and women with preterm delivery at Jimma University Medical Center (JUMC), southwest Ethiopia.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A cross-sectional study was done among 144 women from June to August 2019. Isolates were tested for antibiotic susceptibility and biofilm formation using disc diffusion and microtiter plate method, respectively. Data were collected using a structured questionnaire, entered into Epidata\u00a03.1 and logistic regression was done by SPSS v25.0.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>LM was isolated in 8 (5.56%) of 144 screened women. The isolation rate of LM was relatively higher among women with a history of fetal loss (9.7%), followed by women with preterm delivery (6.25%). One of the six cord blood was positive for LM, indicating that the transplacental transmission rate at JUMC was 16.7%. More than 2% of women with an ongoing pregnancy were found to have LM septicemia, which could hurt their fetus. All of the isolates tested were susceptible to Ampicillin. However, all of the isolates were resistant to Penicillin and Meropenem and were biofilm producers.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The high magnitude of pregnancy-related listeriosis in the current study setting appears that implementation of educational programs targeting risk reduction and more studies to identify sources of LM are warranted. The choice of antibiotics should be after susceptibility testing.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12879-021-06254-w","type":"journal-article","created":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T16:02:46Z","timestamp":1623513766000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia"],"prefix":"10.1186","volume":"21","author":[{"given":"Lencho","family":"Girma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alene","family":"Geteneh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demisew","family":"Amenu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tesfaye","family":"Kassa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,12]]},"reference":[{"key":"6254_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2013\/851712","volume":"2013","author":"T Mateus","year":"2013","unstructured":"Mateus T, Silva J, Maia RL, Teixeira P. Listeriosis during pregnancy: a public health concern. ISRN Obstet Gynecol. 2013;2013:1\u20136. https:\/\/doi.org\/10.1155\/2013\/851712.","journal-title":"ISRN Obstet Gynecol"},{"key":"6254_CR2","volume-title":"WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. Geneva, Switzerland","author":"World Health Organization (WHO)","year":"2015","unstructured":"World Health Organization (WHO). WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. Geneva, Switzerland; 2015."},{"issue":"9","key":"6254_CR3","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1111\/j.1469-0691.2011.03604.x","volume":"17","author":"D Baud","year":"2011","unstructured":"Baud D, Greub G. Intracellular bacteria and adverse pregnancy outcomes. Clin Microbiol Infect. 2011;17(9):1312\u201322. https:\/\/doi.org\/10.1111\/j.1469-0691.2011.03604.x.","journal-title":"Clin Microbiol Infect"},{"key":"6254_CR4","volume-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013: European Food Safety Authority","author":"EFSA","year":"2015","unstructured":"EFSA. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013: European Food Safety Authority; 2015."},{"key":"6254_CR5","first-page":"16","volume":"5","author":"Y Amene","year":"2016","unstructured":"Amene Y, Firesbhat A. Listeriosis in large ruminants: a review. Acad J Animal Dis. 2016;5:16\u201321.","journal-title":"Acad J Animal Dis"},{"key":"6254_CR6","volume-title":"Clinical Practice Guideline Listeriosis in pregnancy","author":"Institute of Obstetricians and Gynaecologists","year":"2018","unstructured":"Institute of Obstetricians and Gynaecologists. Clinical Practice Guideline Listeriosis in pregnancy. Ireland: Royal College of Physicians of Ireland; 2018."},{"key":"6254_CR7","volume-title":"Listeriosis. CDNA National Guidelines for Public Health Units","author":"Communicable Diseases Network Australia (CDNA)","year":"2018","unstructured":"Communicable Diseases Network Australia (CDNA). Listeriosis. CDNA National Guidelines for Public Health Units. 2018."},{"issue":"1","key":"6254_CR8","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1111\/j.1469-0691.2009.03109.x","volume":"16","author":"F Allerberger","year":"2010","unstructured":"Allerberger F, Wagner M. Listeriosis: a resurgent foodborne infection. Clin Microbiol Infect. 2010;16(1):16\u201323. https:\/\/doi.org\/10.1111\/j.1469-0691.2009.03109.x.","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"6254_CR9","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1590\/S1517-83822007000200019","volume":"38","author":"EG Lemes-Marques","year":"2007","unstructured":"Lemes-Marques EG, Cruz CD, Destro MT. Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the state of Sao Paulo, Brazil. Braz J Microbiol. 2007;38(2):287\u201392. https:\/\/doi.org\/10.1590\/S1517-83822007000200019.","journal-title":"Braz J Microbiol"},{"issue":"3","key":"6254_CR10","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1515\/jpm.2011.035","volume":"39","author":"RF Lamont","year":"2011","unstructured":"Lamont RF, Sobel J, Mazaki-Tovi S, Kusanovic JP, Vaisbuch E, Kim SK, et al. Listeriosis in human pregnancy: a systematic review. J Perinat Med. 2011;39(3):227\u201336. https:\/\/doi.org\/10.1515\/jpm.2011.035.","journal-title":"J Perinat Med"},{"issue":"1","key":"6254_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-018-32574-0","volume":"8","author":"A Kuch","year":"2018","unstructured":"Kuch A, Goc A, Belkiewicz K, et al. Molecular diversity and antimicrobial susceptibility of Listeria monocytogenes isolates from invasive infections in Poland (1997\u20132013). Sci Rep. 2018;8(1):1\u201311.","journal-title":"Sci Rep"},{"key":"6254_CR12","volume-title":"Jimma University Medical Center governing Board reviewed 2010 E.C. Performance and approved 2011 E.C. plan. Jimma University","author":"Jimma University Medical Center","year":"2018","unstructured":"Jimma University Medical Center. Jimma University Medical Center governing Board reviewed 2010 E.C. Performance and approved 2011 E.C. plan. Jimma University; 2018."},{"issue":"1","key":"6254_CR13","first-page":"207","volume":"33","author":"M Silaen","year":"2017","unstructured":"Silaen M, Yerizel E, Syukur S, et al. The prevalence of Listeria monocytogenes in placental tissue from abortion and fetal death at mother and Child\u2019s Sri Ratu hospital, Medan, Indonesia. Am Sci Res J Eng Technol Sci (ASRJETS). 2017;33(1):207\u201316.","journal-title":"Am Sci Res J Eng Technol Sci (ASRJETS)"},{"issue":"4","key":"6254_CR14","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1590\/S1984-82502012000400011","volume":"48","author":"TM Benetti","year":"2012","unstructured":"Benetti TM, Monteiro CLB, Beux MR, Abrah\u00e3o WM. Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria species in refrigerated sausages. Brazilian J Pharm Sci. 2012;48(4):677\u201381. https:\/\/doi.org\/10.1590\/S1984-82502012000400011.","journal-title":"Brazilian J Pharm Sci"},{"issue":"6","key":"6254_CR15","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S1684-1182(10)60075-8","volume":"43","author":"SL Huang","year":"2010","unstructured":"Huang SL, Chou YT, Hsieh YC, Huang YC, Lin TY, Chiu CH. Epidemiology and clinical characteristics of Listeria monocytogenes bacteremia in a Taiwanese medical center. J Microbiol Immunol Infect. 2010;43(6):485\u201390. https:\/\/doi.org\/10.1016\/S1684-1182(10)60075-8.","journal-title":"J Microbiol Immunol Infect"},{"key":"6254_CR16","first-page":"781","volume":"1","author":"L Alexandre","year":"2018","unstructured":"Alexandre L, Thomas H, Anna O, et al. Listeria monocytogenes. Princ Pract Pediatr Infect Dis. 2018;1:781\u20135.","journal-title":"Princ Pract Pediatr Infect Dis"},{"key":"6254_CR17","volume-title":"Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious Bacteria. 3rd ed CLSI guideline M45","year":"2016","unstructured":"CLSI, editor. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious Bacteria. 3rd ed CLSI guideline M45. Wayne: Clinical and Laboratory Standards Institute; 2016."},{"key":"6254_CR18","volume-title":"Breakpoint tables for interpretation of MICs and zone diameters. 2018, version 8.0","author":"Testing ECoAS","year":"2018","unstructured":"Testing ECoAS. Breakpoint tables for interpretation of MICs and zone diameters. 2018, version 8.0. 2018."},{"issue":"6","key":"6254_CR19","doi-asserted-by":"publisher","first-page":"2950","DOI":"10.1128\/AEM.68.6.2950-2958.2002","volume":"68","author":"D Djordjevic","year":"2002","unstructured":"Djordjevic D, Wiedmann M, CLandsborough L. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol. 2002;68(6):2950\u20138. https:\/\/doi.org\/10.1128\/AEM.68.6.2950-2958.2002.","journal-title":"Appl Environ Microbiol"},{"key":"6254_CR20","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.lwt.2017.03.045","volume":"81","author":"A Henriques","year":"2017","unstructured":"Henriques A, Fraqueza M. Biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from the ready-to-eat meat-based food products food chain. LWT-Food Sci Technol. 2017;81:180\u20137. https:\/\/doi.org\/10.1016\/j.lwt.2017.03.045.","journal-title":"LWT-Food Sci Technol"},{"key":"6254_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2017\/6479121","volume":"2017","author":"V Filipello","year":"2017","unstructured":"Filipello V, Amato E, Gori M, Huedo P, Ciceri G, Lomonaco S, et al. Epidemiology and molecular typing of pregnancy-associated listeriosis cases in Lombardy, Italy, over a 10-year period (2005\u20132014). Infect Dis Obstet Gynecol. 2017;2017:1\u20138. https:\/\/doi.org\/10.1155\/2017\/6479121.","journal-title":"Infect Dis Obstet Gynecol"},{"issue":"5","key":"6254_CR22","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1111\/j.1365-2672.2007.03414.x","volume":"103","author":"S Kaur","year":"2007","unstructured":"Kaur S, Malik SV, Vaidya VM, et al. Listeria monocytogenes in spontaneous abortions in humans and its detection by multiplex PCR. J Appl Microbiol. 2007;103(5):1889\u201396. https:\/\/doi.org\/10.1111\/j.1365-2672.2007.03414.x.","journal-title":"J Appl Microbiol"},{"key":"6254_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2015\/201479","volume":"2015","author":"M Imanishi","year":"2015","unstructured":"Imanishi M, Routh JA, Klaber M, Gu W, Vanselow MS, Jackson KA, et al. Estimating the attack rate of pregnancy-associated listeriosis during a large outbreak. Infect Dis Obstet Gynecol. 2015;2015:1\u20135. https:\/\/doi.org\/10.1155\/2015\/201479.","journal-title":"Infect Dis Obstet Gynecol"},{"issue":"2","key":"6254_CR24","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1007\/s10096-019-03739-0","volume":"39","author":"NS Fall","year":"2020","unstructured":"Fall NS, Sarr M, Diagne N, Bass\u00e8ne H, Sokhna C, Lagier JC, et al. Listeria monocytogenes detected in vaginal self-samples of 2 women after spontaneous miscarriage, Senegal, West Africa. Eur J Clin Microbiol Infect Dis. 2020;39(2):393\u20134. https:\/\/doi.org\/10.1007\/s10096-019-03739-0.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"2013","key":"6254_CR25","first-page":"18","volume":"25","author":"J Shindang","year":"2013","unstructured":"Shindang J, Shindang CO, Ekwempu AI. Incidence of Listeria monocytogenes and other Bacteria in spontaneous abortion cases in Jos. Nigerian J Biotechnol. 2013;25(2013):18\u201322.","journal-title":"Nigerian J Biotechnol"},{"issue":"1","key":"6254_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-019-4566-8","volume":"12","author":"LN Welekidan","year":"2019","unstructured":"Welekidan LN, Bahta YW, Teklehaimanot MG, et al. Prevalence and drug resistance pattern of Listeria monocytogenes among pregnant women in Tigray region, northern Ethiopia: a cross-sectional study. BMC Res Notes. 2019;12(1):1\u20136.","journal-title":"BMC Res Notes"},{"issue":"4","key":"6254_CR27","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/S0740-0020(02)00140-5","volume":"20","author":"B Dhanashreea","year":"2003","unstructured":"Dhanashreea B, Ottab SK, Karunasagarb I, et al. Incidence of Listeria species in clinical and food samples in Mangalore, India. Food Microbiol. 2003;20(4):447\u201353. https:\/\/doi.org\/10.1016\/S0740-0020(02)00140-5.","journal-title":"Food Microbiol"},{"issue":"9","key":"6254_CR28","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1007\/s12275-015-5243-9","volume":"53","author":"DK Soni","year":"2015","unstructured":"Soni DK, Singh DV, Dubey SK. Pregnancy - associated human listeriosis: virulence and genotypic analysis of Listeria monocytogenes from clinical samples. J Microbiol. 2015;53(9):653\u201360. https:\/\/doi.org\/10.1007\/s12275-015-5243-9.","journal-title":"J Microbiol"},{"issue":"2","key":"6254_CR29","first-page":"1","volume":"17","author":"AI Ernest","year":"2015","unstructured":"Ernest AI, Ng\u2019Walida N, Ndaboine E, et al. Maternal vaginorectal colonization by group B Streptococcus and Listeria monocytogenes and its risk factors among pregnant women attending tertiary hospital in Mwanza, Tanzania. Tanzania J Health Res. 2015;17(2):1\u20139.","journal-title":"Tanzania J Health Res"},{"issue":"1","key":"6254_CR30","doi-asserted-by":"publisher","first-page":"12","DOI":"10.5603\/DEMJ.a2020.0002","volume":"5","author":"K Skowron","year":"2020","unstructured":"Skowron K, Bro\u017cek K, \u0141ukasik M, Wiktorczyk N, Korkus J, Gospodarek-Komkowska E. Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes. Disaster Emerg Med J. 2020;5(1):12\u20138. https:\/\/doi.org\/10.5603\/DEMJ.a2020.0002.","journal-title":"Disaster Emerg Med J"},{"key":"6254_CR31","first-page":"1","volume":"1","author":"S Heidarzadeh","year":"2021","unstructured":"Heidarzadeh S, Pourmand MR, Hasanvand S, et al. Antimicrobial Susceptibility, Serotyping, and Molecular Characterization of Antibiotic Resistance Genes in Listeria monocytogenes Isolated from Pregnant Women with a History of Abortion. Iran J Public Health. 2021;1:1.","journal-title":"Iran J Public Health"},{"issue":"2","key":"6254_CR32","first-page":"1","volume":"6","author":"G Babu","year":"2017","unstructured":"Babu G, Balamuruganvelu S, Maulingkar S, et al. Incidence rate and antibiotic susceptibility pattern of Listeria Species in high risk groups. Natl J Lab Med. 2017;6(2):1\u20135.","journal-title":"Natl J Lab Med"},{"issue":"9","key":"6254_CR33","doi-asserted-by":"publisher","first-page":"e0137046","DOI":"10.1371\/journal.pone.0137046","volume":"10","author":"S Doijad","year":"2015","unstructured":"Doijad S, Barbuddhe S, Garg S, et al. Biofilm-forming abilities of Listeria monocytogenes serotypes isolated from different sources. PLoS One. 2015;10(9):e0137046. https:\/\/doi.org\/10.1371\/journal.pone.0137046.","journal-title":"PLoS One"},{"issue":"2","key":"6254_CR34","first-page":"25","volume":"1","author":"U Tasneem","year":"2018","unstructured":"Tasneem U, Yasin N, Nisa I, et al. Biofilm producing bacteria: A serious threat to public health in developing countries. J Food Sci Nutr. 2018;1(2):25\u201331.","journal-title":"J Food Sci Nutr"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-021-06254-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12879-021-06254-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-021-06254-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T16:03:57Z","timestamp":1623513837000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/s12879-021-06254-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,12]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["6254"],"URL":"https:\/\/doi.org\/10.1186\/s12879-021-06254-w","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,12]]},"assertion":[{"value":"15 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical clearance was obtained from Jimma University IRB with reference number: IHRPGD\/563\/2018. Informed written consent was obtained from each study participant, and in case of participants having no formal education, informed consent was obtained from their legal guardians. Lab finding was disclosed to the study participant and their health care provider. Helsinki declarations were used as guiding principles during this research activity.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declared that no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"564"}}