{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T12:27:34Z","timestamp":1782563254119,"version":"3.54.5"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007194","name":"Wenzhou science and Technology Bureau","doi-asserted-by":"crossref","award":["Y20160251"],"award-info":[{"award-number":["Y20160251"]}],"id":[{"id":"10.13039\/501100007194","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To investigate the correlation between pleural fluid interleukin-33 (IL-33) and adenosine deaminase (ADA) and peripheral blood tuberculosis T cell spot detection (T-SPOT.TB), and the combined value of the three tests for the diagnosis of tuberculous pleurisy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>79 patients with pleural effusion admitted from June 2017 to December 2018 were enrolled. They were divided into tuberculous pleural effusion (TPE) group (57 cases, 72.2%) and malignant pleural effusion group (17 cases, 21.5%), pneumonia-like pleural effusion group (5 cases, 6.3%). Correlation between pleural fluid IL-33, pleural effusion ADA and peripheral blood T-SPOT.TB was analyzed, comparison of the three separate and combined diagnostic efficacy was also performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The levels of IL-33, ADA and peripheral blood T-SPOT.TB in patients with TPE were significantly higher than those in non-TPE (P\u2009&lt;\u20090.001). The level of pleural fluid IL-33 was positively correlated with pleural effusion ADA and peripheral blood T-SPOT.TB. The Area under the ROC curve (AUC) of TPE diagnosed by pleural IL-33, ADA and peripheral blood T-SPOT.TB were 0.753, 0.912 and 0.865, respectively. AUC for combined detection of pleural effusion IL-33, ADA and peripheral blood T-SPOT.TB is the largest, with a value of 0.962. Specificity is 100% and sensitivity is 88.5%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Combined detection of pleural effusion IL-33, ADA and peripheral blood T-SPOT.TB can improve the diagnostic efficacy of tuberculous pleurisy.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12879-021-06575-w","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T09:21:55Z","timestamp":1629710515000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Diagnostic value of combined pleural interleukin-33, adenosine deaminase and peripheral blood tuberculosis T cell spot detection TB for tuberculous pleurisy"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8757-5170","authenticated-orcid":false,"given":"Jin","family":"Fenhua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Daohui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Hui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Xiaodong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0010-3056","authenticated-orcid":false,"given":"Huang","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"issue":"47","key":"6575_CR1","doi-asserted-by":"publisher","first-page":"83315","DOI":"10.18632\/oncotarget.18160","volume":"8","author":"S Xie","year":"2017","unstructured":"Xie S, Lin L, Ming L, et al. The efficacy and safety of adjunctive corticosteroids in the treatment of tuberculosis pleurisy: a systematic review and meta analysis. Oncotarget. 2017;8(47):83315\u201322.","journal-title":"Oncotarget"},{"key":"6575_CR2","doi-asserted-by":"publisher","first-page":"101941","DOI":"10.1016\/j.tube.2020.101941","volume":"122","author":"Y Luo","year":"2020","unstructured":"Luo Y, Xue Y, Guo X, et al. Diagnostic value of pleural fluid T-SPOT for tuberculous pleurisy: an updated meta-analysis. Tuberculosis. 2020;122:101941.","journal-title":"Tuberculosis"},{"key":"6575_CR3","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1186\/s12890-017-0526-z","volume":"17","author":"J Wang","year":"2017","unstructured":"Wang J, et al. The pleural fluid lactate dehydrogenase\/ adenosine deaminase ratio differentiates between tuberculous and parapneumonic pleural effusions. BMC Pulm Med. 2017;17:168.","journal-title":"BMC Pulm Med"},{"issue":"3","key":"6575_CR4","doi-asserted-by":"publisher","first-page":"e0213728","DOI":"10.1371\/journal.pone.0213728","volume":"14","author":"AN Aggarwal","year":"2019","unstructured":"Aggarwal AN, Agarwa LR, Sehgal IS, Dhooria S. Adenosine deaminase for diagnosis of tuberculous pleural effusion: a systematic review and meta-analysis. PLoS ONE. 2019;14(3):e0213728.","journal-title":"PLoS ONE"},{"issue":"17","key":"6575_CR5","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.3760\/cma.j.issn.0366-6999.20130020","volume":"126","author":"Y Wu","year":"2013","unstructured":"Wu Y, Ye Z, Qin S, et al. Combined detections of interleukin 27, interferon-\u03b3, and adenosine deaminase in pleural effusion for diagnosis of tuberculous pleurisy. Chin Med J. 2013;126(17):3215\u201321.","journal-title":"Chin Med J"},{"issue":"1","key":"6575_CR6","first-page":"18","volume":"47","author":"F Zhu","year":"2018","unstructured":"Zhu F, Ou Q, Zheng J. Application values of T-SPOT.TB in clinical rapid diagnosis of tuberculosis. Iran J Public Health. 2018;47(1):18\u201323.","journal-title":"Iran J Public Health"},{"key":"6575_CR7","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1186\/s40249-017-0331-1","volume":"6","author":"L Ji","year":"2017","unstructured":"Ji L, et al. Usefulness of interferon-\u03b3 release assay for the diagnosis of sputum smear-negative pulmonary and extra-pulmonary TB in Zhejiang Province, China. Infect Dis Poverty. 2017;6:121.","journal-title":"Infect Dis Poverty"},{"issue":"1","key":"6575_CR8","first-page":"888","volume":"8","author":"D Li","year":"2015","unstructured":"Li D, Shen Y, Fu X, et al. Combined detections of interleukin-33 and adenosine deaminase for diagnosis of tuberculous pleural effusion. Int J Clin Exp Pathol. 2015;8(1):888\u201393.","journal-title":"Int J Clin Exp Pathol"},{"issue":"1","key":"6575_CR9","doi-asserted-by":"publisher","first-page":"45","DOI":"10.3343\/alm.2013.33.1.45","volume":"33","author":"KS Lee","year":"2013","unstructured":"Lee KS, Kim HR, Kwak S, et al. Association between elevated pleural interleukin-33 levels and tuberculous pleurisy. Ann Lab Med. 2013;33(1):45\u201351.","journal-title":"Ann Lab Med"},{"issue":"1","key":"6575_CR10","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3892\/ol.2014.2109","volume":"8","author":"WX Xuan","year":"2014","unstructured":"Xuan WX, Zhang JC, Zhou Q, et al. IL-33 levels differentiate tuberculous pleurisy from malignant pleural effusions. Oncol Lett. 2014;8(1):449\u201353.","journal-title":"Oncol Lett"},{"issue":"4","key":"6575_CR11","doi-asserted-by":"publisher","first-page":"153","DOI":"10.4046\/trd.2014.76.4.153","volume":"76","author":"D Jeon","year":"2014","unstructured":"Jeon D. Tuberculous pleurisy: an update. Tuberc Respir Dis (Seoul). 2014;76(4):153\u20139.","journal-title":"Tuberc Respir Dis (Seoul)"},{"key":"6575_CR12","doi-asserted-by":"publisher","first-page":"301","DOI":"10.3389\/fmed.2019.00301","volume":"6","author":"X Lei","year":"2019","unstructured":"Lei X, Wang J, Yang Z, et al. Diagnostic value of pleural effusion mononuclear cells count and adenosine deaminase for tuberculous pleurisy patients in China: a case-control study. Front Med (Lausanne). 2019;6:301.","journal-title":"Front Med (Lausanne)"},{"issue":"5","key":"6575_CR13","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/j.immuni.2005.09.015","volume":"23","author":"J Schmitz","year":"2005","unstructured":"Schmitz J, Owyang A, Oldham E, et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2005;23(5):479\u201390.","journal-title":"Immunity"},{"issue":"9","key":"6575_CR14","doi-asserted-by":"publisher","first-page":"4331","DOI":"10.4049\/jimmunol.1103685","volume":"189","author":"L Blom","year":"2012","unstructured":"Blom L, Poulsen LK. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189(9):4331\u20137.","journal-title":"J Immunol"},{"issue":"8","key":"6575_CR15","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1093\/intimm\/dxn060","volume":"20","author":"MD Smithgall","year":"2008","unstructured":"Smithgall MD, Comeau MR, Yoon BR, et al. IL-33 amplifies both Th1- and Th2-type responses through its activity on human basophils, allergen-reactive Th2 cells, iNKT and NK cells. Int Immunol. 2008;20(8):1019\u201330.","journal-title":"Int Immunol"},{"issue":"4","key":"6575_CR16","doi-asserted-by":"publisher","first-page":"1046","DOI":"10.1002\/eji.200838575","volume":"39","author":"E Bourgeois","year":"2009","unstructured":"Bourgeois E, Van LP, Samson M, et al. The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production. Eur J Immunol. 2009;39(4):1046\u201355.","journal-title":"Eur J Immunol"},{"issue":"1","key":"6575_CR17","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.bbrc.2009.04.081","volume":"384","author":"IS Wood","year":"2009","unstructured":"Wood IS, Wang B, Trayhurn P. IL-33, a recently identified interleukin-1 gene family member, is expressed in human adipocytes. Biochem Biophys Res Commun. 2009;384(1):105\u20139.","journal-title":"Biochem Biophys Res Commun"},{"issue":"4","key":"6575_CR18","doi-asserted-by":"publisher","first-page":"G821","DOI":"10.1152\/ajpgi.00178.2010","volume":"299","author":"A Masamune","year":"2010","unstructured":"Masamune A, Watanabe T, Kikuta K, et al. Nuclear expression of interleukin-33 in pancreatic stellate cells. Am J Physiol Gastrointest Liver Physiol. 2010;299(4):G821-832.","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"31","key":"6575_CR19","doi-asserted-by":"publisher","first-page":"10913","DOI":"10.1073\/pnas.0801898105","volume":"105","author":"D Xu","year":"2008","unstructured":"Xu D, Jiang HR, Kewin P, et al. IL-33 exacerbates antigen-induced arthritis by activating mast cells. Proc Natl Acad Sci USA. 2008;105(31):10913\u20138.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"8","key":"6575_CR20","doi-asserted-by":"publisher","first-page":"5094","DOI":"10.4049\/jimmunol.0802387","volume":"183","author":"D Pr\u00e9fontaine","year":"2009","unstructured":"Pr\u00e9fontaine D, Lajoie-Kadoch S, Foley S, et al. Increased expression of IL-33 in severe asthma: evidence of expression by airway smooth muscle cells. J Immunol. 2009;183(8):5094\u2013103.","journal-title":"J Immunol"},{"key":"6575_CR21","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1016\/j.rmed.2007.12.007","volume":"102","author":"QL Liang","year":"2008","unstructured":"Liang QL, Shi HZ, Wang K, Qin SM, Qin XJ. Diagnostic accuracy of adenosine deaminase in tuberculous pleurisy: a meta-analysis. Respir Med. 2008;102:744\u201354.","journal-title":"Respir Med"},{"key":"6575_CR22","doi-asserted-by":"publisher","first-page":"2251","DOI":"10.2169\/internalmedicine.0387-17","volume":"57","author":"A Nagayasu","year":"2018","unstructured":"Nagayasu A, Kubo S, Nakano K, et al. IgG4-related pleuritis with elevated adenosine deaminase in pleural effusion. Intern Med. 2018;57:2251\u20137.","journal-title":"Intern Med"},{"issue":"44","key":"6575_CR23","doi-asserted-by":"publisher","first-page":"e5200","DOI":"10.1097\/MD.0000000000005200","volume":"95","author":"XQ Liu","year":"2016","unstructured":"Liu XQ, Bian SN, Cheng XH, et al. Utility of T-cell interferon-\u03b3 release assays for the diagnosis of female genital tuberculousis in a tertiary referral hospital in Beijing, China. Medicine (Baltimore). 2016;95(44):e5200.","journal-title":"Medicine (Baltimore)"},{"issue":"1","key":"6575_CR24","doi-asserted-by":"publisher","first-page":"345","DOI":"10.3892\/etm.2015.2463","volume":"10","author":"L Yan","year":"2015","unstructured":"Yan L, Xiao H, Han M, et al. Diagnostic value of T-SPOT.TB interferon-\u03b3 release assays for active tuberculosis. Exp Ther Med. 2015;10(1):345\u201351.","journal-title":"Exp Ther Med"},{"issue":"4","key":"6575_CR25","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.jinf.2014.12.018","volume":"70","author":"L Pan","year":"2015","unstructured":"Pan L, Jia H, Liu F, et al. Risk factors for false-negative TSPOT.TB assay results in patients with pulmonary and extrapulmonary TB. J Infect. 2015;70(4):367\u201380.","journal-title":"J Infect"},{"issue":"2","key":"6575_CR26","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1055\/s-0036-1572559","volume":"37","author":"MK Haas","year":"2016","unstructured":"Haas MK, Daley CL. Mycobacterial lung disease complicating HIV infection. Semin Respir Crit Care Med. 2016;37(2):230\u201342.","journal-title":"Semin Respir Crit Care Med"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-021-06575-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12879-021-06575-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-021-06575-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T02:46:43Z","timestamp":1725677203000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/s12879-021-06575-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,23]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["6575"],"URL":"https:\/\/doi.org\/10.1186\/s12879-021-06575-w","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,23]]},"assertion":[{"value":"2 August 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 August 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The protocol was approved by the second affiliated hospital of Wenzhou Medical university research ethics committee with Certificate number L-2020-01. Written consent was provided by TB patients for whom clinical and demographic data were extracted.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that there is no conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"861"}}