{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:52:22Z","timestamp":1790293942449,"version":"4.1.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T00:00:00Z","timestamp":1632614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T00:00:00Z","timestamp":1632614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Hematology analyzers display abnormal parameters during malaria infection providing insightful information for suspecting and assessing malaria infection. The goal of this study is to demonstrate the potential of a three-part differential hematology analyzer to assess malaria, provide information about the parasitemia, and discuss the importance of combining C-reactive protein (CRP) with hematology parameters to obtain further information about the malaria infection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The present study shows the results of a case\u2013control study during the monsoon season of years 2018 and 2019 in Mumbai, India. The study considers 1008 non-malaria febrile cases, 209 <jats:italic>P. vivax<\/jats:italic> and 31 <jats:italic>P. falciparum<\/jats:italic> positive malaria samples, five cases of mixed <jats:italic>P. vivax<\/jats:italic> and <jats:italic>P. falciparum<\/jats:italic> infection, and three co-infection cases of <jats:italic>P. vivax<\/jats:italic> and dengue. Raw data from the three-part analyzer LC-667G CRP (HORIBA) and the corresponding microscopic findings (golden standard for diagnosis of malaria) were obtained for each sample.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The medians of platelet counts (PLT) were 102.5, 109.0, and 223.0\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\/\u00b5L, while CRP medians were 67.4, 81.4 and 10.4\u00a0mg\/L in <jats:italic>P. vivax<\/jats:italic>, <jats:italic>P. falciparum<\/jats:italic> and control groups respectively (p\u2009&lt;\u20090.001 in Mann\u2013Whitney U tests between malaria and control groups). Compared with negative samples, platelets counting less than 161.5\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\/\u00b5L were observed on malaria patients (OR 19.12, 95% CI 11.89\u201330.75). Especially in <jats:italic>P. vivax<\/jats:italic> cases, an abnormal peak was frequently observed in the white blood cells (WBC) histogram around the 37fL channel. The events counted around that channel showed a linear correlation with the counting of red blood cells infected predominantly with larger parasitic forms. Parameters like CRP (rs\u2009=\u20090.325, p\u2009&lt;\u20090.001), WBC (rs\u2009=\u20090.285, p\u2009&lt;\u20090.001) and PLT (rs\u2009=\u2009\u2212\u20090.303, p\u2009&lt;\u20090.001) were correlated with the parasitemia of <jats:italic>P. vivax<\/jats:italic> samples. Between the malaria and dengue groups, the highest area under the receiver operating characteristic curve was observed on CRP (0.867, CRP\u2009\u2265\u200926.85\u00a0mg\/L).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A three-part differential hematology analyzer has the potential to not only trigger malaria diagnosis confirmation but also assess the severity of the infection when CRP is considered.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12879-021-06704-5","type":"journal-article","created":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T21:04:34Z","timestamp":1632690274000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The utility of basic blood counts, WBC histogram and C-reactive protein in detecting malaria"],"prefix":"10.1186","volume":"21","author":[{"given":"Jun","family":"Nishimura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parag","family":"Dharap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Raimbault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,26]]},"reference":[{"key":"6704_CR1","unstructured":"WHO. 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Ethical clearance was not obtained as the study was carried out as a retrospective review of the available raw instrument and historical patient data, without any collection of extra specimen or monetary charges to patients and did not involve any communication of results of the study to the clinician, so as to affect the diagnostic and therapeutic management of the patients.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"JN and SR are employees of HORIBA Ltd. PD is an external consultant of HORIBA Ltd.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1006"}}