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Several serological approaches have been developed using recombinant antigens to improve the diagnostic indices of serological tests for melioidosis.\n<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Fusion proteins from <jats:italic>Burkholderia pseudomallei<\/jats:italic> (rGroEL-FLAG300) were evaluated as a potential target antigen for melioidosis antibodies. A total of 220 serum samples from 38 culture proven melioidosis patients (gold standard), 126 healthy individuals from endemic (n\u2009=\u200937) and non-endemic (n\u2009=\u200989) Thai provinces and 56 patients with other proven bacterial infections as negative controls were tested using indirect enzyme-linked immunosorbent assays (ELISA).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Using an optical density (OD) cut-off of 0.299148, our assay had 94.74% sensitivity (95% confidence interval (CI)\u2009=\u200982.3\u201399.4%), 95.05% specificity (95% CI\u2009=\u200990.8\u201397.7%), and 95% accuracy, which was better than in our previous work (90.48% sensitivity, 87.14% specificity, and 87.63% accuracy).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our results suggest that the application of chimeric antigens in ELISA could improve the serological diagnosis of melioidosis and should be reconfirmed with greater patient numbers.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12879-022-07369-4","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T13:04:47Z","timestamp":1650373487000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enhanced serodiagnosis of melioidosis by indirect ELISA using the chimeric protein rGroEL-FLAG300 as an antigen"],"prefix":"10.1186","volume":"22","author":[{"given":"Sumet","family":"Wajanarogana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Water R. 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The need for individual informed consent was waived by the Ethics Committee of Faculty of Medicine, Vajira Hospital, Navamindradhiraj University (no. COA 63\/2558) because we used anonymized, stored samples from previously approved study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no completing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Completing interests"}}],"article-number":"387"}}