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The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of <jats:italic>Toxoplasma gondii<\/jats:italic> isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-<jats:italic>T. gondii<\/jats:italic> IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients\u2019 buffy coats. Anti-<jats:italic>T. gondii<\/jats:italic> IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-<jats:italic>T. gondii<\/jats:italic> IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to <jats:italic>T. gondii<\/jats:italic> carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored.<\/jats:p>","DOI":"10.1186\/s12879-024-09265-5","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T06:02:26Z","timestamp":1713333746000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022"],"prefix":"10.1186","volume":"24","author":[{"given":"Nasim","family":"Bashour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arash","family":"Aminpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shabnam","family":"Vazifehkhah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasool","family":"Jafari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,4,17]]},"reference":[{"key":"9265_CR1","doi-asserted-by":"crossref","unstructured":"Su C, Dubey JP. 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The study was approved by the Ethics Committee of Urmia University of Medical Sciences under the Ethical Code of IR.UMSU.REC.1401.031. Participants were informed about the study and a signed informed consent was obtained from them.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"410"}}