{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:48:47Z","timestamp":1777906127573,"version":"3.51.4"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"DOI":"10.1186\/s12879-024-10194-6","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T09:24:17Z","timestamp":1734513857000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis"],"prefix":"10.1186","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7906-7034","authenticated-orcid":false,"given":"Reza","family":"Saberi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5877-2845","authenticated-orcid":false,"given":"Homa","family":"Hajjaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4690-6938","authenticated-orcid":false,"given":"Mahdi","family":"Fakhar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9656-1854","authenticated-orcid":false,"given":"Zahra","family":"Mirabedini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4164-9514","authenticated-orcid":false,"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"10194_CR1","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s40475-021-00232-7","volume":"8","author":"S Mann","year":"2021","unstructured":"Mann S, Frasca K, Scherrer S, Henao-Mart\u00ednez AF, Newman S, Ramanan P, et al. A review of leishmaniasis: current knowledge and future directions. Curr Trop Med Rep. 2021;8:121\u201332.","journal-title":"Curr Trop Med Rep"},{"issue":"7","key":"10194_CR2","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.adengl.2021.05.011","volume":"112","author":"I Abad\u00edas-Granado","year":"2021","unstructured":"Abad\u00edas-Granado I, Diago A, Cerro P, Palma-Ruiz A, Gilaberte Y. Cutaneous and mucocutaneous leishmaniasis. Actas Dermo Sifiliogr\u00e1ficas (English Edition). 2021;112(7):601\u201318.","journal-title":"Actas Dermo Sifiliogr\u00e1ficas (English Edition)"},{"issue":"6","key":"10194_CR3","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1111\/tbed.13316","volume":"66","author":"R Saberi","year":"2019","unstructured":"Saberi R, Fakhar M, Mohebali M, Anvari D, Gholami S. Global status of synchronizing Leishmania RNA virus in Leishmania parasites: a systematic review with meta-analysis. Transbound Emerg Dis. 2019;66(6):2244\u201351.","journal-title":"Transbound Emerg Dis"},{"issue":"15","key":"10194_CR4","doi-asserted-by":"publisher","first-page":"5979","DOI":"10.1073\/pnas.86.15.5979","volume":"86","author":"G Widmer","year":"1989","unstructured":"Widmer G, Comeau AM, Furlong DB, Wirth DF, Patterson JL. Characterization of a RNA virus from the parasite Leishmania. Proc Natl Acad Sci. 1989;86(15):5979\u201382.","journal-title":"Proc Natl Acad Sci"},{"issue":"10","key":"10194_CR5","doi-asserted-by":"publisher","first-page":"830","DOI":"10.3390\/genes10100830","volume":"10","author":"Y Kleschenko","year":"2019","unstructured":"Kleschenko Y, Grybchuk D, Matveeva NS, Macedo DH, Ponirovsky EN, Lukashev AN, et al. Molecular characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan. Genes. 2019;10(10):830.","journal-title":"Genes"},{"issue":"12","key":"10194_CR6","doi-asserted-by":"publisher","first-page":"2300","DOI":"10.1093\/nar\/23.12.2300","volume":"23","author":"G Widmer","year":"1995","unstructured":"Widmer G, Dooley S. Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association. Nucleic Acids Res. 1995;23(12):2300\u20134.","journal-title":"Nucleic Acids Res"},{"key":"10194_CR7","doi-asserted-by":"publisher","first-page":"e01932","DOI":"10.1128\/mBio.01932-18","volume":"9","author":"D Grybchuk","year":"2018","unstructured":"Grybchuk D, Kostygov A, Macedo D, Votypka J, Lukes J, Yurchenko V. RNA viruses in Blechomonas (Trypanosomatidae) and evolution of Leishmaniavirus. mBio. 2018;9:e01932\u201318.","journal-title":"mBio"},{"key":"10194_CR8","doi-asserted-by":"publisher","unstructured":"Louten J. Virus structure and classification. Essential Hum Virol. 2016;6:19\u201329. https:\/\/doi.org\/10.1016\/B978-0-12-800947-5.00002-8.","DOI":"10.1016\/B978-0-12-800947-5.00002-8"},{"key":"10194_CR9","doi-asserted-by":"publisher","unstructured":"Proch\u00e1zkov\u00e1 M, F\u00fczik T, Grybchuk D, Falginella FL, Pode\u0161vov\u00e1 L, Yurchenko V, et al. Capsid structure of Leishmania RNA virus 1. J Virol. 2021;95(3):https:\/\/doi.org\/10.1128\/jvi.01957-20.","DOI":"10.1128\/jvi.01957-20"},{"issue":"10","key":"10194_CR10","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1017\/S0031182017001810","volume":"145","author":"LF Paranaiba","year":"2018","unstructured":"Paranaiba LF, Pinheiro LJ, Macedo DH, Menezes-Neto A, Torrecilhas AC, Tafuri WL, et al. An overview on Leishmania (Mundinia) enriettii: biology, immunopathology, LRV and extracellular vesicles during the host\u2013parasite interaction. Parasitology. 2018;145(10):1265\u201373.","journal-title":"Parasitology"},{"key":"10194_CR11","doi-asserted-by":"publisher","first-page":"99","DOI":"10.3389\/fcimb.2012.00099","volume":"2","author":"M-A Hartley","year":"2012","unstructured":"Hartley M-A, Ronet C, Zangger H, Beverley SM, Fasel N. Leishmania RNA virus: when the host pays the toll. Front Cell Infect Microbiol. 2012;2:99.","journal-title":"Front Cell Infect Microbiol"},{"issue":"6018","key":"10194_CR12","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1126\/science.1199326","volume":"331","author":"A Ives","year":"2011","unstructured":"Ives A, Ronet C, Prevel F, Ruzzante G, Fuertes-Marraco S, Schutz F, et al. Leishmania RNA virus controls the severity of mucocutaneous leishmaniasis. Science. 2011;331(6018):775\u20138.","journal-title":"Science"},{"issue":"1","key":"10194_CR13","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1186\/s12866-023-03140-0","volume":"23","author":"Z Mirabedini","year":"2023","unstructured":"Mirabedini Z, Mirjalali H, Kazemirad E, Khamesipour A, Samimirad K, Koosha M, et al. The effects of Leishmania RNA virus 2 (LRV2) on the virulence factors of L. major and pro-inflammatory biomarkers: an in vitro study on human monocyte cell line (THP-1). BMC Microbiol. 2023;23(1):398.","journal-title":"BMC Microbiol."},{"issue":"11","key":"10194_CR14","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.3390\/v13112305","volume":"13","author":"AY Kostygov","year":"2021","unstructured":"Kostygov AY, Grybchuk D, Kleschenko Y, Chistyakov DS, Lukashev AN, Gerasimov ES, et al. Analyses of Leishmania-LRV co-phylogenetic patterns and evolutionary variability of viral proteins. Viruses. 2021;13(11):2305.","journal-title":"Viruses"},{"issue":"1","key":"10194_CR15","doi-asserted-by":"publisher","first-page":"14","DOI":"10.4103\/0972-9062.234621","volume":"55","author":"R Saberi","year":"2018","unstructured":"Saberi R, Moin-Vaziri V, Hajjaran H, Niyyati M, Taghipour N, Kheirandish F, et al. Identification of Leishmania species using N-acetylglucosamine-1-phosphate transferase gene in a zoonotic cutaneous leishmaniasis focus of Iran. J Vector Borne Dis. 2018;55(1):14\u20139.","journal-title":"J Vector Borne Dis"},{"key":"10194_CR16","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.ijid.2020.08.033","volume":"101","author":"R Saberi","year":"2020","unstructured":"Saberi R, Fakhar M, Hajjaran H, Ataei-Pirkooh A, Mohebali M, Taghipour N, et al. Presence and diversity of Leishmania RNA virus in an old zoonotic cutaneous leishmaniasis focus, northeastern Iran: haplotype and phylogenetic based approach. Int J Infect Dis. 2020;101:6\u201313.","journal-title":"Int J Infect Dis"},{"issue":"6","key":"10194_CR17","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","volume":"35","author":"S Kumar","year":"2018","unstructured":"Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547.","journal-title":"Mol Biol Evol"},{"issue":"2","key":"10194_CR18","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1093\/molbev\/msh018","volume":"21","author":"D Bryant","year":"2004","unstructured":"Bryant D, Moulton V. Neighbor-net: an agglomerative method for the construction of phylogenetic networks. Mol Biol Evol. 2004;21(2):255\u201365.","journal-title":"Mol Biol Evol"},{"key":"10194_CR19","unstructured":"Sverdlov E, Azhikina T. Primer walking. e LS. 2001."},{"issue":"2","key":"10194_CR20","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1006\/viro.1994.1149","volume":"199","author":"S Scheffter","year":"1994","unstructured":"Scheffter S, Widmer G, Patterson JL. Complete sequence of Leishmania RNA virus 1\u20134 and identification of conserved sequences. Virology. 1994;199(2):479\u201383.","journal-title":"Virology"},{"issue":"1","key":"10194_CR21","doi-asserted-by":"publisher","first-page":"109","DOI":"10.2353\/jmoldx.2010.090035","volume":"12","author":"R Stadhouders","year":"2010","unstructured":"Stadhouders R, Pas SD, Anber J, Voermans J, Mes TH, Schutten M. The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5\u2032 nuclease assay. J Mol Diagn. 2010;12(1):109\u201317.","journal-title":"J Mol Diagn"},{"issue":"1","key":"10194_CR22","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1186\/s12915-023-01687-y","volume":"21","author":"DH Macedo","year":"2023","unstructured":"Macedo DH, Grybchuk D, Re\u017enarov\u00e1 J, Vot\u00fdpka J, Klocek D, Yurchenko T, et al. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris. BMC Biol. 2023;21(1):191.","journal-title":"BMC Biol"},{"issue":"7","key":"10194_CR23","doi-asserted-by":"publisher","first-page":"e0005764","DOI":"10.1371\/journal.pntd.0005764","volume":"11","author":"S Tirera","year":"2017","unstructured":"Tirera S, Ginouves M, Donato D, Caballero IS, Bouchier C, Lavergne A, et al. Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana. PLoS Negl Trop Dis. 2017;11(7):e0005764.","journal-title":"PLoS Negl Trop Dis"},{"key":"10194_CR24","first-page":"3385","volume":"161","author":"H Hajjaran","year":"2016","unstructured":"Hajjaran H, Mahdi M, Mohebali M, Samimi-Rad K, Ataei-Pirkooh A, Kazemi-Rad E, et al. Detection and molecular identification of Leishmania RNA virus (LRV) in Iranian Leishmania species. Adv Virol. 2016;161:3385\u201390.","journal-title":"Adv Virol"},{"issue":"1","key":"10194_CR25","doi-asserted-by":"publisher","first-page":"e2006","DOI":"10.1371\/journal.pntd.0002006","volume":"7","author":"H Zangger","year":"2013","unstructured":"Zangger H, Ronet C, Desponds C, Kuhlmann FM, Robinson J, Hartley M-A, et al. Detection of Leishmania RNA virus in Leishmania parasites. PLoS Negl Trop Dis. 2013;7(1):e2006.","journal-title":"PLoS Negl Trop Dis"},{"key":"10194_CR26","doi-asserted-by":"publisher","first-page":"105222","DOI":"10.1016\/j.meegid.2022.105222","volume":"98","author":"M Farrokhi-Karibozorg","year":"2022","unstructured":"Farrokhi-Karibozorg M, Ghayour-Najafabadi Z, Hejazi SH, Ataei-Pirkooh A, Mohebali M, Teimouri P, et al. Molecular identification of Leishmania RNA virus in cutaneous leishmaniasis patients and rodent reservoirs in Isfahan province, Iran. Infect Genet Evol. 2022;98:105222.","journal-title":"Infect Genet Evol"},{"issue":"10","key":"10194_CR27","doi-asserted-by":"publisher","first-page":"2279","DOI":"10.1007\/s00436-023-07928-x","volume":"122","author":"D Klocek","year":"2023","unstructured":"Klocek D, Grybchuk D, Tich\u00e1 L, Vot\u00fdpka J, Volf P, Kostygov AY, et al. Evolution of RNA viruses in trypanosomatids: new insights from the analysis of Sauroleishmania. Parasitol Res. 2023;122(10):2279\u201386.","journal-title":"Parasitol Res"},{"issue":"3","key":"10194_CR28","doi-asserted-by":"publisher","first-page":"e0004349","DOI":"10.1371\/journal.pntd.0004349","volume":"10","author":"M Akhoundi","year":"2016","unstructured":"Akhoundi M, Kuhls K, Cannet A, Vot\u00fdpka J, Marty P, Delaunay P, et al. A historical overview of the classification, evolution, and dispersion of Leishmania parasites and sandflies. PLoS Negl Trop Dis. 2016;10(3):e0004349.","journal-title":"PLoS Negl Trop Dis"},{"key":"10194_CR29","doi-asserted-by":"publisher","unstructured":"Grybchuk D, Kostygov AY, Macedo DH, Vot\u00fdpka J, Luke\u0161 J, Yurchenko V. RNA viruses in Blechomonas (Trypanosomatidae) and evolution of Leishmaniavirus. MBio. 2018;9(5):https:\/\/doi.org\/10.1128\/mbio.01932-18.","DOI":"10.1128\/mbio.01932-18"},{"issue":"6","key":"10194_CR30","doi-asserted-by":"publisher","first-page":"e0198727","DOI":"10.1371\/journal.pone.0198727","volume":"13","author":"LM Cantanh\u00eade","year":"2018","unstructured":"Cantanh\u00eade LM, Fernandes FG, Ferreira GEM, Porrozzi R, Ferreira RdGM, Cupolillo E. New insights into the genetic diversity of Leishmania RNA Virus 1 and its species-specific relationship with Leishmania parasites. PLoS One. 2018;13(6):e0198727.","journal-title":"PLoS One."}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-024-10194-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12879-024-10194-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-024-10194-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T10:03:56Z","timestamp":1734516236000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/s12879-024-10194-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["10194"],"URL":"https:\/\/doi.org\/10.1186\/s12879-024-10194-6","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,18]]},"assertion":[{"value":"1 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The current research was conducted with approval from the ethical principles and national regulations governing medical research (IR.MAZUMS.REC.1398.344). All experiments were performed in accordance with relevant guidelines and regulations. Informed written consent was obtained from adult participants and the parents of children, adhering to guidelines, and their responses were documented using a structured questionnaire.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1407"}}