{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:05:56Z","timestamp":1790229956273,"version":"4.1.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002386","name":"Cairo University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002386","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Introduction<\/jats:title>\n            <jats:p>Rapid and accurate pathogen identification is critical and has a major impact on sepsis-related mortality and morbidity rates. The aim of this study is to evaluate the performance of a simple, fast, and inexpensive method for direct processing of positive blood cultures for immediate identification of microorganisms using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>This study was performed on 125 positive blood culture bottles collected from April 2022 to September 2023. We compared using MALDI-TOF MS for identifying isolated microorganisms from positive blood culture bottles on routine culture media versus identifying microorganisms directly from positive blood culture bottles without previous isolation on routine culture media.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The total number of microorganisms isolated by routine subculture methods was 128, as three bottles had two types of organisms. Out of the 128 organisms isolated by routine subculture method, 97 (75.8%) organisms were identified to species level using the direct detection method from blood culture bottles by MALDI-TOF MS. 4 (3.1%) organisms were identified to genus level only, while 3 (2.3%) were wrongly identified. This direct method could not identify 24 (18.8%) organisms. Gram-negative organisms were identified with high accuracy, achieving 90.16% (55\/61) at the species level and 3.28% (2\/61) at the genus level. Among Gram-positive organisms, 69.1% (38\/55) were identified to the species level, while 27.3% (15\/55) could not be identified. Yeast identification was limited, with only 33.3% (4\/12) identified to the species level, 8.3% (1\/12) to the genus level, and 41.7% (5\/12) could not be identified.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This method showed great sensitivity for the identification of Gram-negative bacteria, directly from positive blood culture bottles, followed by Gram-positive bacteria, and very low sensitivity for yeasts.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12879-025-11129-5","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T13:03:53Z","timestamp":1748005433000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid and cost-effective identification of microorganisms from positive blood cultures using MALDI-TOF MS"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7189-5401","authenticated-orcid":false,"given":"Maryam Mostafa","family":"Ashmawy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar Mohamed","family":"Khairat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nashwa Mohamed","family":"Reda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7852-3045","authenticated-orcid":false,"given":"Mona Moheyeldin","family":"Abdelhalim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"issue":"4","key":"11129_CR1","doi-asserted-by":"publisher","first-page":"e23119","DOI":"10.1002\/jcla.23119","volume":"34","author":"Y Yuan","year":"2020","unstructured":"Yuan Y, Wang J, Zhang J, Ma B, Gao S, Li Y, et al. Evaluation of an optimized method to directly identify bacteria from positive blood cultures using MALDI-TOF mass spectrometry. J Clin Lab Anal. 2020;34(4):e23119. https:\/\/doi.org\/10.1002\/jcla.23119.","journal-title":"J Clin Lab Anal"},{"issue":"1","key":"11129_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-019-4861-4","volume":"13","author":"WAMP Samaranayake","year":"2020","unstructured":"Samaranayake WAMP, Dempsey S, Howard-Jones AR, Outhred AC, Kesson AM. Rapid direct identification of positive pediatric blood cultures by MALDI-TOF MS technology and its clinical impact in the pediatric hospital setting. 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This study did not directly involve human participants. It utilized anonymized clinical specimens, and the need for informed consent was waived by the Research Ethics Committee (REC) of the Faculty of Medicine at Cairo University.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"736"}}