{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:56:55Z","timestamp":1781585815414,"version":"3.54.5"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Natural Science Foundation of Shandong Province in China","award":["ZR2022MH095"],"award-info":[{"award-number":["ZR2022MH095"]}]},{"name":"Natural Science Foundation of Shandong Province in China","award":["ZR2023MH030"],"award-info":[{"award-number":["ZR2023MH030"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Nephrol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Ureteropelvic junction obstruction (UPJO) is the most common cause of pediatric congenital hydronephrosis, and continuous kidney function monitoring plays a role in guiding the treatment of UPJO. In this study, we aimed to explore the differentially expressed proteins (DEPs) in the urinary extracellular vesicles(uEVs) of children with UPJO and determine potential biomarkers of uEVs proteins that reflect kidney function changes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Preoperative urine samples from 6 unilateral UPJO patients were collected and divided into two groups: differential renal function (DRF)\u2009\u2265\u200940% and DRF\u2009&lt;\u200940%.We subsequently used data-independent acquisition (DIA) to identify and quantify uEVs proteins in urine, screened for DEPs between the two groups, and analyzed biofunctional enrichment information. The proteomic data were evaluated by Western blotting and enzyme-linked immunosorbent assay (ELISA) in a new UPJO testing cohort.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>After one-way ANOVA, a <jats:italic>P<\/jats:italic> adj value\u2009&lt;\u20090.05 (<jats:italic>P<\/jats:italic>-value corrected by Benjamin\u2013Hochberg) was taken, and the absolute value of the difference multiple was more than 1.5 as the screening basis for obtaining 334 DEPs. After analyzing the enrichment of the DEPs according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment combined with the protein\u2013protein interaction (PPI) network results, we selected nicotinamide adenine dinucleotide-ubiquinone oxidoreductase core subunit S1 (NDUFS1) for further detection. The expression of NDUFS1 in uEVs was significantly lower in patients with DRF\u2009&lt;\u200940% (1.182\u2009\u00b1\u20090.437 vs. 1.818\u2009\u00b1\u20090.489, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and the expression level of NDUFS1 was correlated with the DRF in the affected kidney (<jats:italic>r<\/jats:italic>\u2009=\u20090.78, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). However, the NDUFS1 concentration in intravesical urine was not necessarily related to the change in DRF (<jats:italic>r<\/jats:italic>\u2009=\u20090.28, <jats:italic>P<\/jats:italic>\u2009=\u20090.24).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Reduced expression of NDUFS1 in uEVs might indicate the decline of DRF in children with UPJO.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12882-024-03592-0","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T14:02:28Z","timestamp":1715176948000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Association between NDUFS1 from urinary extracellular vesicles and decreased differential renal function in children with ureteropelvic junction obstruction"],"prefix":"10.1186","volume":"25","author":[{"given":"Lingyun","family":"Bu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongde","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"issue":"2","key":"3592_CR1","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/S0022-5347(17)43157-0","volume":"138","author":"SA Koff","year":"1987","unstructured":"Koff SA. Problematic ureteropelvic junction obstruction. J Urol. 1987;138(2):390.","journal-title":"J Urol"},{"issue":"Suppl 1","key":"3592_CR2","doi-asserted-by":"publisher","first-page":"S41","DOI":"10.1007\/s00247-007-0590-2","volume":"38","author":"SA Koff","year":"2008","unstructured":"Koff SA. Requirements for accurately diagnosing chronic partial upper urinary tract obstruction in children with hydronephrosis. Pediatr Radiol. 2008;38(Suppl 1):S41\u20138.","journal-title":"Pediatr Radiol"},{"issue":"8","key":"3592_CR3","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1016\/j.jpedsurg.2010.03.030","volume":"45","author":"Y Yang","year":"2010","unstructured":"Yang Y, Hou Y, Niu ZB, Wang CL. Long-term follow-up and management of prenatally detected, isolated hydronephrosis. J Pediatr Surg. 2010;45(8):1701\u20136. https:\/\/doi.org\/10.1016\/j.jpedsurg.2010.03.030.","journal-title":"J Pediatr Surg"},{"issue":"4","key":"3592_CR4","doi-asserted-by":"publisher","first-page":"504.e501","DOI":"10.1016\/j.jpurol.2022.06.017","volume":"18","author":"Y Li","year":"2022","unstructured":"Li Y, He Y, Zhang W, Song H, Wang T. Factors predicting improvement of differential renal function after pyeloplasty in children of ureteropelvic junction obstruction. J Pediatr Urol. 2022;18(4):504.e501\u2013504.e506. https:\/\/doi.org\/10.1016\/j.jpurol.2022.06.017.","journal-title":"J Pediatr Urol"},{"issue":"11","key":"3592_CR5","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1038\/ki.2011.292","volume":"80","author":"BWM van Balkom","year":"2011","unstructured":"van Balkom BWM, Pisitkun T, Verhaar MC, Knepper MA. Exosomes and the kidney: prospects for diagnosis and therapy of kidney diseases. Kidney Int. 2011;80(11):1138\u201345. https:\/\/doi.org\/10.1038\/ki.2011.292.","journal-title":"Kidney Int"},{"issue":"36","key":"3592_CR6","doi-asserted-by":"publisher","first-page":"13368","DOI":"10.1073\/pnas.0403453101","volume":"101","author":"T Pisitkun","year":"2004","unstructured":"Pisitkun T, Shen R-F, Knepper MA. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004;101(36):13368\u201373.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"3592_CR7","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s00467-017-3816-z","volume":"34","author":"A-L St\u00e5hl","year":"2019","unstructured":"St\u00e5hl A-L, Johansson K, Mossberg M, Kahn R, Karpman D. Exosomes and microvesicles in normal physiology, pathophysiology, and kidney diseases. Pediatr Nephrol. 2019;34(1):11\u201330. https:\/\/doi.org\/10.1007\/s00467-017-3816-z.","journal-title":"Pediatr Nephrol"},{"issue":"R1","key":"3592_CR8","doi-asserted-by":"publisher","first-page":"R125","DOI":"10.1093\/hmg\/dds317","volume":"21","author":"Y Lee","year":"2012","unstructured":"Lee Y, El Andaloussi S, Wood MJA. Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy. Hum Mol Genet. 2012;21(R1):R125\u201334.","journal-title":"Hum Mol Genet"},{"key":"3592_CR9","doi-asserted-by":"publisher","unstructured":"Cricr\u00ec G, Bellucci L, Montini G, Collino F: Urinary Extracellular Vesicles: Uncovering the Basis of the Pathological Processes in Kidney-Related Diseases. Int J Mol Sci. 2021, 22(12).https:\/\/doi.org\/10.3390\/ijms22126507","DOI":"10.3390\/ijms22126507"},{"issue":"3","key":"3592_CR10","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1515\/cclm-2015-0599","volume":"54","author":"RJ Hendriks","year":"2016","unstructured":"Hendriks RJ, Dijkstra S, Jannink SA, Steffens MG, van Oort IM, Mulders PFA, Schalken JA. Comparative analysis of prostate cancer specific biomarkers PCA3 and ERG in whole urine, urinary sediments and exosomes. Clin Chem Lab Med. 2016;54(3):483\u201392. https:\/\/doi.org\/10.1515\/cclm-2015-0599.","journal-title":"Clin Chem Lab Med"},{"key":"3592_CR11","doi-asserted-by":"publisher","first-page":"504","DOI":"10.3389\/fped.2020.00504","volume":"8","author":"Q Wang","year":"2020","unstructured":"Wang Q, Shi Z, Xing X, Deng Y, Li W, Xie T, Jiang D. Matrix remodeling-associated protein 5 in urinary exosomes as a potential novel marker of obstructive nephropathy in children with Ureteropelvic junction obstruction. Front Pediatr. 2020;8:504. https:\/\/doi.org\/10.3389\/fped.2020.00504.","journal-title":"Front Pediatr"},{"issue":"2","key":"3592_CR12","doi-asserted-by":"publisher","first-page":"e12404","DOI":"10.1002\/jev2.12404","volume":"13","author":"JA Welsh","year":"2024","unstructured":"Welsh JA, Goberdhan DCI, O\u2019Driscoll L, Buzas EI, Blenkiron C, Bussolati B, Cai H, Di Vizio D, Driedonks TAP, Erdbr\u00fcgger U, et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024;13(2):e12404. https:\/\/doi.org\/10.1002\/jev2.12404.","journal-title":"J Extracell Vesicles"},{"issue":"3","key":"3592_CR13","doi-asserted-by":"publisher","first-page":"e12286","DOI":"10.1002\/jev2.12286","volume":"12","author":"ME van Royen","year":"2023","unstructured":"van Royen ME, Soekmadji C, Grange C, Webber JP, Tertel T, Droste M, Buescher A, Giebel B, Jenster GW, Llorente A, et al. The quick reference card \u201cStorage of urinary EVs\u201d - A practical guideline tool for research and clinical laboratories. J Extracell Vesicles. 2023;12(3):e12286. https:\/\/doi.org\/10.1002\/jev2.12286.","journal-title":"J Extracell Vesicles"},{"issue":"3","key":"3592_CR14","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1111\/bju.15689","volume":"130","author":"A Rai","year":"2022","unstructured":"Rai A, Hsieh A, Smith A. Contemporary diagnosis and management of pelvi-ureteric junction obstruction. BJU Int. 2022;130(3):285\u201390. https:\/\/doi.org\/10.1111\/bju.15689.","journal-title":"BJU Int"},{"issue":"4","key":"3592_CR15","doi-asserted-by":"publisher","first-page":"e1800101","DOI":"10.1002\/prca.201800101","volume":"13","author":"H Chen","year":"2019","unstructured":"Chen H, Lin H, Xu M, Xu G, Fang X, He L, Chen Z, Wu Z, Geng H. Quantitative urinary proteome reveals potential biomarkers for ureteropelvic junction obstruction. Proteomics Clin Appl. 2019;13(4):e1800101. https:\/\/doi.org\/10.1002\/prca.201800101.","journal-title":"Proteomics Clin Appl"},{"issue":"6","key":"3592_CR16","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1016\/j.cell.2016.01.043","volume":"164","author":"M Tkach","year":"2016","unstructured":"Tkach M, Th\u00e9ry C. Communication by extracellular vesicles: where we are and where we need to go. Cell. 2016;164(6):1226\u201332. https:\/\/doi.org\/10.1016\/j.cell.2016.01.043.","journal-title":"Cell"},{"key":"3592_CR17","doi-asserted-by":"publisher","first-page":"31357","DOI":"10.1038\/srep31357","volume":"6","author":"C-Y Su","year":"2016","unstructured":"Su C-Y, Chang Y-C, Yang C-J, Huang M-S, Hsiao M. The opposite prognostic effect of NDUFS1 and NDUFS8 in lung cancer reflects the oncojanus role of mitochondrial complex I. Sci Rep. 2016;6:31357. https:\/\/doi.org\/10.1038\/srep31357.","journal-title":"Sci Rep"},{"issue":"2","key":"3592_CR18","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1038\/ki.2015.332","volume":"89","author":"O Ruiz-Andres","year":"2016","unstructured":"Ruiz-Andres O, Suarez-Alvarez B, S\u00e1nchez-Ramos C, Monsalve M, Sanchez-Ni\u00f1o MD, Ruiz-Ortega M, Egido J, Ortiz A, Sanz AB. The inflammatory cytokine TWEAK decreases PGC-1\u03b1 expression and mitochondrial function in acute kidney injury. Kidney Int. 2016;89(2):399\u2013410. https:\/\/doi.org\/10.1038\/ki.2015.332.","journal-title":"Kidney Int"},{"key":"3592_CR19","doi-asserted-by":"publisher","unstructured":"Ni Y, Hagras MA, Konstantopoulou V, Mayr JA, Stuchebrukhov AA, Meierhofer D: Mutations in NDUFS1 Cause Metabolic Reprogramming and Disruption of the Electron Transfer. Cells. 2019, 8(10).https:\/\/doi.org\/10.3390\/cells8101149","DOI":"10.3390\/cells8101149"},{"issue":"7","key":"3592_CR20","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1038\/s12276-022-00800-5","volume":"54","author":"B Qi","year":"2022","unstructured":"Qi B, Song L, Hu L, Guo D, Ren G, Peng T, Liu M, Fang Y, Li C, Zhang M, et al. Cardiac-specific overexpression of Ndufs1 ameliorates cardiac dysfunction after myocardial infarction by alleviating mitochondrial dysfunction and apoptosis. Exp Mol Med. 2022;54(7):946\u201360. https:\/\/doi.org\/10.1038\/s12276-022-00800-5.","journal-title":"Exp Mol Med"},{"key":"3592_CR21","doi-asserted-by":"publisher","unstructured":"Elkholi R, Abraham-Enachescu I, Trotta AP, Rubio-Pati\u00f1o C, Mohammed JN, Luna-Vargas MPA, Gelles JD, Kaminetsky JR, Serasinghe MN, Zou C et al: MDM2 Integrates Cellular Respiration and Apoptotic Signaling through NDUFS1 and the Mitochondrial Network. Mol Cell 2019, 74(3).https:\/\/doi.org\/10.1016\/j.molcel.2019.02.012","DOI":"10.1016\/j.molcel.2019.02.012"}],"container-title":["BMC Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12882-024-03592-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12882-024-03592-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12882-024-03592-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T14:02:57Z","timestamp":1715176977000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcnephrol.biomedcentral.com\/articles\/10.1186\/s12882-024-03592-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,8]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["3592"],"URL":"https:\/\/doi.org\/10.1186\/s12882-024-03592-0","relation":{},"ISSN":["1471-2369"],"issn-type":[{"value":"1471-2369","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,8]]},"assertion":[{"value":"4 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental protocol was established according to the ethical guidelines of the.The Helsinki Declaration was approved by the Human Ethics Committee of Shandong Provincial Hospital (SWYX: No. 2023\u2013403). Written informed consent was obtained from the individuals or guardian participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"158"}}