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The targeting relationship between NLRP3 and miR-223-3P was verified by bioinformatics analysis and classical double-luciferase reporter gene assay. Lipopolysaccharide (LPS) was used to induce HTR8\/SVneo cells as PE placental cell inflammation model. Then we transfected miR-223-3p overexpression\/miR-223-3p negative control plasmid into the LPS-induced HTR8\/SVneo cells. Next, the expressions of NLRP3, Caspase-1, GSDMD, IL-1\u03b2 and IL-18 were evaluated to elucidate the regulatory effect of miR-223-3p on the inflammatory response mediated by NLRP3 in PE placenta.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Compared with normal controls, NLRP3 was significantly up-regulated in PE placenta, while miR-223-3p was down-regulated. In addition, NLRP3 was a direct target of miR-223-3p. Further research revealed that the expression of NLRP3, Caspase-1, GSDMD, IL-1\u03b2 and IL-18 could be obviously promoted in HTR8\/SVneo cells treated with LPS (500 ng\/ml) for 24\u00a0h, nevertheless it could be significantly suppressesed under the overexpression of miR-223-3p.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>MiR-223-3p suppressed NLRP3 inflamariomes activation, downstream inflammatory factors secretion and pyroptosis in LPS-induced HTR8\/SVneo cells indicating that miR-223-3p could serve as an anti-inflammatory factor in preeclampsia.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12884-024-06371-9","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T13:02:21Z","timestamp":1709730141000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3"],"prefix":"10.1186","volume":"24","author":[{"given":"Xueqiong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"issue":"9461","key":"6371_CR1","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/S0140-6736(05)17987-2","volume":"365","author":"B Sibai","year":"2005","unstructured":"Sibai B, Dekker G, Kupferminc M. 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All subjects provided informed consent prior to their inclusion in the study. All the procedures performed in this study were in accordance with the principles of the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"175"}}