{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:40:26Z","timestamp":1782135626886,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Xiamen Science and Technology Bureau Science and Technology Benefits People Program","award":["3502Z20184045"],"award-info":[{"award-number":["3502Z20184045"]}]},{"name":"Xiamen Medical and Health Guidance Project","award":["3502Z20209195"],"award-info":[{"award-number":["3502Z20209195"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Pregnancy Childbirth"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Previous studies have reported inconsistent results regarding blastocyst selection with a high day 3 (D3) cell number and the eventual pregnancy outcomes. Thus, in this study, the relationship between the D3 cell number and clinical outcomes of day 5 single blastocyst transfer (SBT) in vitrified-warmed transfer cycles was investigated.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Our retrospective study included 1144\u00a0day 5 SBT in vitrified-warmed cycles between February 2016 and February 2021. All cycles were the first vitrified-warmed cycles, and the female patients were less than 35 years of age. Based on the D3 cell number, the cycles were divided into four groups, as follows: group A (3\u20137 cells, <jats:italic>n<\/jats:italic>\u2009=\u2009130); group B (8\u20139 cells, <jats:italic>n<\/jats:italic>\u2009=\u2009621); group C (10\u201312 cells, <jats:italic>n<\/jats:italic>\u2009=\u2009328); and group D (13\u201316 cells, <jats:italic>n<\/jats:italic>\u2009=\u200965). The differences in the live birth rate (LBR), clinical pregnancy rate, and miscarriage rate were examined among the four groups.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The LBR and clinical pregnancy rate increased with the D3 cell number (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). No significant difference was found in the miscarriage rate among the groups (<jats:italic>P<\/jats:italic>\u2009=\u20090.055). After adjusting for confounding factors, the LBR was significantly higher in groups C (odds ratio [OR]\u2009=\u20091.477, 95% confidence interval [CI]: 1.124\u20131.941, <jats:italic>P<\/jats:italic>\u2009=\u20090.005) and D (OR\u2009=\u20092.000, 95% CI: 1.166\u20133.429, <jats:italic>P<\/jats:italic>\u2009=\u20090.012) than in group B.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A high D3 cell number (&gt;\u20099 cells) was associated with a high LBR in the vitrified-warmed day 5 SBT cycles of patients\u2009&lt;\u200935 years of age. The cell number of D3 embryos can be an important reference indicator for blastocyst selection. Among blastocysts with the same morphological score, those with &gt;\u20099 cells on D3 can be preferentially selected for transplantation.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12884-024-06468-1","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T07:01:53Z","timestamp":1713510113000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Day 3 cell number on the live birth rate of vitrified-warmed Day 5 single blastocyst transfer in young women"],"prefix":"10.1186","volume":"24","author":[{"given":"Pingping","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghui","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"key":"6468_CR1","doi-asserted-by":"publisher","unstructured":"Glujovsky D, Farquhar C. 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PMID 21995143.","journal-title":"Clin Exp Obstet Gynecol"}],"container-title":["BMC Pregnancy and Childbirth"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12884-024-06468-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12884-024-06468-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12884-024-06468-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T07:04:01Z","timestamp":1713510241000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcpregnancychildbirth.biomedcentral.com\/articles\/10.1186\/s12884-024-06468-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,19]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["6468"],"URL":"https:\/\/doi.org\/10.1186\/s12884-024-06468-1","relation":{},"ISSN":["1471-2393"],"issn-type":[{"value":"1471-2393","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,19]]},"assertion":[{"value":"26 February 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 March 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 April 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was approved by the Ethics Committee of Xiamen Women and Children\u2019s Hospital (Approval number: KY-2022-057-K01) and was in line with the Declaration of Helsinki. Informed patient consent was not required as the study was retrospective in nature and analyzed patient data anonymously. A statement from the Ethics Committee of Xiamen Women and Children\u2019s Hospital waived the need for informed consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"289"}}