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Colitis associated colorectal adenocarcinoma (CAC) often shows distinct morphologic and molecular phenotypes compared to sporadic cases. However, the expression profile of LGR5, and by extension the potential role of an intestinal stem cell phenotype, has not been well described in a series of human CAC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      RNA in situ hybridization (ISH) for\n                      <jats:italic>LGR5<\/jats:italic>\n                      expression on 30 CACs (12 cases with conventional morphology and 18 cases with non-conventional type morphology) from 29 inflammatory bowel disease (IBD) patients was performed and compared the expression profile to a control group of 10 sporadic CRCs. Immunohistochemistry for beta-catenin and SATB2 was performed on the 30 CACs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      <jats:italic>LGR5<\/jats:italic>\n                      was positive in 30% (9\/30) of CAC cases and 90% (9\/10) of sporadic CRCs (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002). A large majority (89%) of\n                      <jats:italic>LGR5<\/jats:italic>\n                      positive CACs were of the conventional histologic type, and conventional type CAC showed a significantly higher\n                      <jats:italic>LGR5<\/jats:italic>\n                      score (median 3.0; interquartile range 1.75\u20133.25) than non-conventional type CAC (median 1.5; interquartile range 1.00\u20132.00) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.034). CAC with conventional morphology did have a lower level of LGR5 expression than sporadic CRC. Sporadic CRCs showed a significantly higher LGR5 level score than non-conventional type CACs (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Nuclear translocation of beta-catenin was strongly associated with\n                      <jats:italic>LGR5<\/jats:italic>\n                      expression (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.003), however no significant association was identified between SATB2 expression and\n                      <jats:italic>LGR5<\/jats:italic>\n                      expression status in CACs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that the wider spectrum of tumor morphology in CAC may be associated with absence of a\n                      <jats:italic>LGR5<\/jats:italic>\n                      -expressing intestinal stem cell phenotype.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12885-021-07835-3","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T12:19:03Z","timestamp":1612441143000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Most colitis associated carcinomas lack expression of LGR5: a preliminary study with implications for unique pathways of carcinogenesis compared to sporadic colorectal carcinoma"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2135-2219","authenticated-orcid":false,"given":"Mai","family":"Iwaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyoshi","family":"Ota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Uehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Riddell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Conner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"issue":"4","key":"7835_CR1","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1136\/gut.48.4.526","volume":"48","author":"JA Eaden","year":"2001","unstructured":"Eaden JA, Abrams KR, Mayberry JF. 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