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Infection with specific human papillomavirus (HPV) types is considered the most severe risk factor for cervical cancer. In the context of our socioeconomic conditions, an increasing burden of this disease and high mortality rate prevail in Bangladesh. Although several researches related to the epidemiology, HPV vaccination, and treatment modalities were conducted, researches on the mutation profiles of marker genes in cervical cancer in Bangladesh remain unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, five different genomic regions within the top three most frequently mutated genes (\n                      <jats:italic>EGFR, KRAS<\/jats:italic>\n                      and\n                      <jats:italic>PIK3CA)<\/jats:italic>\n                      in COSMIC database with a key role in the development of cervical cancers were selected to study the mutation frequency in Bangladeshi patients.\n                      <jats:italic>In silico<\/jats:italic>\n                      analysis was done in two steps: nucleotide sequence analysis and its corresponding amino acid analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DNA from 46 cervical cancer tissue samples were extracted and amplified by PCR, using 1 set of primers designed for\n                      <jats:italic>EGFR<\/jats:italic>\n                      and 2 sets of primers designed for two different regions of both\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      and\n                      <jats:italic>KRAS<\/jats:italic>\n                      gene. In total, 39 mutations were found in 26 patient samples. Eleven different mutations (23.91%), twenty-four different mutations (52.17%) and four mutations (8.7%) were found in amplified\n                      <jats:italic>EGFR, PIK3CA<\/jats:italic>\n                      and\n                      <jats:italic>KRAS<\/jats:italic>\n                      gene fragments,\u00a0respectively; among which 1 (\n                      <jats:italic>EGFR<\/jats:italic>\n                      ) was common in seven patient samples and 2 (\n                      <jats:italic>PIKCA<\/jats:italic>\n                      ) were found in more than 1 patient. Our study shows that except for\n                      <jats:italic>KRAS<\/jats:italic>\n                      , the frequency of observed mutations in our patients is higher than those reported earlier in other parts of the world. Most of the exonic mutations were found only in the\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      and\n                      <jats:italic>EGFR<\/jats:italic>\n                      genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The study can be used as a basis to build a mutation database for cervical cancer in Bangladesh with the possibility of targetable oncogenic mutations. Further explorations are\u00a0needed to establish future diagnostics, personalized medicine decisions, and other pharmaceutical applications for specific cancer subtypes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12885-021-07906-5","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T18:16:06Z","timestamp":1616091366000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mutational profiles of marker genes of cervical carcinoma in Bangladeshi patients"],"prefix":"10.1186","volume":"21","author":[{"given":"Shahana","family":"Sharmin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima Tuj","family":"Zohura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. 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Institutional ethical clearance was taken from National Institute of Cancer Research and Hospital (NICHR), Mohakhali, Dhaka (Ref. no: NICRH\/Ethics\/2014\/115). Institutional review board (IRB) clearance was taken from Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka (No. BSMMU\/2017\/151). Ethical Clearance Committee of the University of Dhaka also approved all protocols (Ethical Clearance No. 30\/Bio.Fac.\/2016\u20132017). Before collection of the tissue sample, included every patients\u2019 written consents were obtained using a consent form.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no conflict of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"289"}}