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In 305 eyes of 305 patients without infective eye diseases who underwent ocular surgeries at the First Affiliated Hospital of Harbin Medical University between May 2018 and May 2019, conjunctival sac scrapings were collected on the day before surgery.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The mean age of the participants was 60.73\u2009\u00b1\u200914.23\u2009years, with the majority being female, married and unemployed with at least primary school education. The positive culture rate was 48.20% (147\/305 eyes), and 191 bacterial strains were isolated; two or more strains were isolated from 22.45% (33\/147) of positive samples. The most commonly isolated bacteria was <jats:italic>Staphylococcus epidermidis<\/jats:italic> (64.92%), surpassing <jats:italic>Staphylococcus aureus<\/jats:italic> (5.76%). The culture positive rate of the young (18\u201340\u2009years) group was different between the females (26.67%) and males (69.23%) (<jats:italic>P<\/jats:italic>\u2009=\u20090.024), but in the middle-aged group and the elderly group, the rates between the sexes were similar, with an increasing trend. Patients who visited in summer or autumn presented a higher positive rate than other seasons. Hypertensive women had a higher rate than hypertensive men (58.14% vs. 40%, \u03c7<jats:sup>2<\/jats:sup>\u2009=\u20095.<jats:italic>8662, P<\/jats:italic>\u2009=\u20090.0154).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In northeastern China, coagulase-negative <jats:italic>Staphylococcus<\/jats:italic> were the most common preoperative conjunctival bacteria<jats:italic>.<\/jats:italic> Hypertensive female patients, elderly patients, or those operated on in the summer and autumn should pay more attention to perioperative treatment.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Trial registration<\/jats:title>\n                <jats:p><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.chictr.org.cn\/showproj.aspx?proj=123755\">ChiCTR2100044659<\/jats:ext-link>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12886-022-02441-8","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T07:13:28Z","timestamp":1652685208000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bacteriological profile of conjunctiva bacterial Flora in Northeast China: a hospital-based study"],"prefix":"10.1186","volume":"22","author":[{"given":"Shuo","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4179-5666","authenticated-orcid":false,"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"issue":"1","key":"2441_CR1","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1186\/s12887-020-02203-9","volume":"20","author":"R Ke","year":"2020","unstructured":"Ke R, Zhang M, Zhou Q, et al. 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All experimental protocols were approved by the First Affiliated Hospital of Harbin Medical University Ethical Committee (No. 201848) and strictly followed the Helsinki Declaration. All patients provided written informed consent before participating in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Appropriate comment consent was taken from participants.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"223"}}