{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T16:30:24Z","timestamp":1778603424590,"version":"3.51.4"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007051","name":"Uppsala University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007051","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Ophthalmol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purposes<\/jats:title>\n                <jats:p>The aim of this study is to investigate the time evolution of active caspase 3 within first 120\u00a0h in the rat lens after in vivo exposure to subthreshold dose of UVR-B.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Twenty three six-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1 kJ\/m<jats:sup>2<\/jats:sup>) of UVR-B unilaterally and sacrificed at 24, 41, 70 and 120\u00a0h after exposure. Lenses were enucleated and active caspase 3 was detected by Western Blot. The time evolution of active caspase 3 was then plotted as a function of relative mean difference in active caspase 3 between exposed and nonexposed lenses.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>There is expression of active caspase 3 in both exposed and nonexposed lenses but there is no difference in relative mean difference in active caspase 3 between exposed and nonexposed lenses in all four postexposure groups.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Exposure to subthreshold dose of UVR-B does not induce apoptosis in the rat lens in vivo within first 120\u00a0h though there is a non-significant increase of active caspase 3 at 120\u00a0h. Increase in sample size might reduce the variation level in expression of active caspase 3 in the rat lenses.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12886-024-03315-x","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T08:03:07Z","timestamp":1705910587000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of active caspase 3 in the rat lens after in vivo exposure to subthreshold dose of UVR-B"],"prefix":"10.1186","volume":"24","author":[{"given":"Konstantin","family":"Galichanin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohua","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"3315_CR1","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1080\/01926230701320337","volume":"35","author":"S Elmore","year":"2007","unstructured":"Elmore S. 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All animals were treated in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Visual Research. Ethical approval was obtained from the Uppsala Ethics Committee on Animal Experiments, protocol number 5.2.18\u20138927\/16.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29"}}