{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T20:07:25Z","timestamp":1780430845362,"version":"3.54.1"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,5]],"date-time":"2021-06-05T00:00:00Z","timestamp":1622851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,5]],"date-time":"2021-06-05T00:00:00Z","timestamp":1622851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["17ZR1418000"],"award-info":[{"award-number":["17ZR1418000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81270085"],"award-info":[{"award-number":["81270085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Municipal Health Commission","award":["202040164"],"award-info":[{"award-number":["202040164"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Pediatr"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Wheezing is common in younger children and often related to viral infection. It is lack of reliable indicators for asthma prediction.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Objective<\/jats:title>\n                <jats:p>To evaluate the relationship between circulation CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup> memory Th2 cells and asthma diagnosis in wheezing children.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A prospective study was performed in children under 5 years old presented with wheezing or at last one episode of documented wheezing history. After inclusion, the level of serum allergen-specific serum IgE (sIgE) and circulating CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup>cells were detected. The patients\u2019 personal and family histories of allergic disease were acquired by questionnaire. The children were followed up over 2 years. Diagnosis of asthma was assessed at the end follow-up. The risk factors in predicting asthma diagnosis were evaluated.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 43 children completed follow-up. Higher wheezing frequency were found in children with asthma diagnosis. The mean of circulating CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup>cells in children diagnosed with or without asthma was 1.6\u2009%\u00b10.8 and 0.8\u2009%\u00b10.6\u2009%, respectively, and was significantly higher in children diagnosed with asthma (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). There was no significant difference between children with and without allergic diseases history or family allergic diseases in level of circulating CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup> cells. Logistic regression analysis indicated that circulating CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup> cells (EXP, 8.986; 95\u2009% CI,1.886\u201342.816) and wheezing frequency(EXP, 0.127; 95\u2009% CI, 0.023\u20130.703)were high risk factors for asthma.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our exploratory study shown that circulating CD4<jats:sup>+<\/jats:sup>CCR6<jats:sup>+<\/jats:sup>CRTh2<jats:sup>+<\/jats:sup> memory Th2 cells increased in asthma diagnosed children and it was a high-risk factor for asthma. Detection of this type of cells could be helpful in predicting the risk of asthma in wheezing children.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12887-021-02746-5","type":"journal-article","created":{"date-parts":[[2021,6,5]],"date-time":"2021-06-05T06:04:54Z","timestamp":1622873094000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A pilot study to evaluate the role of circulation CD4+ CCR6+ CRTh2+ cell in predicting risk of asthma in wheezing children"],"prefix":"10.1186","volume":"21","author":[{"given":"Jingyang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanhua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingzhu","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoxu","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwei","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,5]]},"reference":[{"issue":"4\u20135","key":"2746_CR1","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1242\/dmm.000323","volume":"1","author":"AT Nials","year":"2008","unstructured":"Nials AT, Uddin S. 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The Ethics Committee at Xinhua Hospital approved the study. The children\u2019s legal guardians signed written informed consent before the research began.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that there is no conflict of interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"263"}}