{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T15:29:33Z","timestamp":1781969373026,"version":"3.54.5"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Pediatr"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sepsis-associated encephalopathy (SAE) is characterized by brain dysfunction during sepsis, without central nervous system infection. Here, we explored the molecular basis of brain injury in preterm infants with SAE. From Jan 2016 to Dec 2019, a total of 20 preterm infants were hospitalized in the neonatal intensive care unit (NICU)\u00a0of our hospital, including 10 preterm infants with SAE (SAE group) and 10 preterm infants without encephalopathy after sepsis (no SAE group). Among the 20 premature infants, there were 12 males and 8 females, with mean gestational age 31.0\u2009\u00b1\u20092.46\u2009weeks, 7 cases with birth weight\u2009\u2264\u20091500\u2009g and 13 cases\u00a0with birth weight 1500\u20132500\u2009g. Blood cultures were negative in 6 cases and positive in 14 cases, including 10 cases of Gram-negative and 4 cases of\u00a0Gram-positive bacteria, respectively. Expression levels of messenger RNA (mRNA) and  MicroRNA\u00a0(miRNA) were analyzed in peripheral blood samples from both groups during sepsis. There were 1858 upregulated and 2226 downregulated mRNAs [fold-change (FC)\u2009&gt;\u2009|2|, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05], and 322 upregulated and 160 downregulated miRNAs (FC\u2009&gt;\u2009|2|, <jats:italic>p\u2009&lt;<\/jats:italic>\u00a00.05), respectively, in the SAE group compared with the no SAE group. Expression levels of miRNA-1197 [95% confidence intervals (CI), 0.042 to 0.166] were 6.03-fold higher in the SAE group than the no SAE group, while those of miRNA-485-5p (95% CI, 0.064 to 0.024) were lower (0.31-fold). Both high expression of miRNA-1197 and low expression of miRNA-485-5p may be associated with pathogenic alteration of the oxidative respiratory chain and energy metabolism in preterm infants with SAE.<\/jats:p>","DOI":"10.1186\/s12887-022-03372-5","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T11:07:00Z","timestamp":1654859220000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The molecular basis of brain injury in preterm infants with sepsis - associated\u00a0encephalopathy"],"prefix":"10.1186","volume":"22","author":[{"given":"Xiaohui","family":"Gong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongbing","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"issue":"2","key":"3372_CR1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1097\/MOP.0000000000000315","volume":"28","author":"JL Wynn","year":"2016","unstructured":"Wynn JL. 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Premature infants with (<i>n<\/i>\u2009=\u200910) and without (<i>n<\/i>\u2009=\u200910) SAE after sepsis were recruited in the NICU of Shanghai Children\u2019s Hospital. All parents of the infants signed the informed consent for the study. This study does not involve personal privacy. The study protocol was approved by Ethics Committee of Shanghai Children\u2019s Hospital (2012RY001-E01), all methods were carried out in accordance with relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"336"}}