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We evaluated anxiety responses and testing intentions among gay, bisexual and other men who have sex with men (GBMSM) using\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      , an artificial intelligence-powered STI risk assessment tool, compared to a standard sexual health information webpage for GBMSMs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a quasi-experimental pre-post study at the Melbourne Sexual Health Centre between April and October 2024. Participants were allocated to either\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      , which provides personalised HIV\/STI risk assessment, or a standard webpage providing general sexual health information for GBMSM on alternating days. We measured anxiety using the State-Trait Anxiety Inventory (STAI-6), which ranges from 6 to 24, before and after participants viewed their assigned websites. We defined clinically significant anxiety changes as \u2265\u20093 points on STAI-6. We used multivariable ordinal logistic regression analysis to evaluate clinically significant anxiety changes while controlling for baseline scores and demographic variables.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our study population had a median age of 34 years (IQR: 28\u201342); 48% were born in Australia, and 69.7% had tertiary education. The baseline characteristics were similar between\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009150) and control (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009150) groups. In the\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      group, STAI-6 scores increased significantly from baseline (median [IQR]: 10 [8, 12]) to post-intervention (11 [8, 13],\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001). The control group showed a decrease from baseline (11 [9, 12]) to post-intervention (10 [8, 13],\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.01). Despite increased anxiety,\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      users maintained high user acceptability (92.0%) with similar testing intentions between groups (87.3% vs. 82.7%,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.6). The multivariable ordinal logistic regression analysis showed that the intervention\u2019s effect on anxiety was independent of demographic characteristics, except for employment status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      While\n                      <jats:italic>MySTIRisk<\/jats:italic>\n                      increased anxiety in some users, it maintained high acceptability and did not deter testing intentions. These findings support implementing such tools with appropriate anxiety management strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12889-025-25064-2","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T11:56:19Z","timestamp":1763466979000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anxiety responses and testing intentions among gay and bisexual men using an AI-powered HIV\/STI risk assessment tool: a quasi-experimental study"],"prefix":"10.1186","volume":"25","author":[{"given":"Phyu Mon","family":"Latt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nyi Nyi","family":"Soe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yining","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason J.","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric P. 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All research procedures adhered to the committee\u2019s ethical standards and regulations, ensuring the safety of participants throughout the study. All participants provided their informed consent at the beginning of the online survey. They could only continue with the survey after providing their consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"PL, NS, XX, YB, LZ and CKF have licensed the use of MySTIRisk to Helfie (Level 5\/171 La Trobe St, Melbourne VIC 3000). XX is an editorial board member for BMC Public Health. The remaining authors declare no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"4028"}}