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Infections in humans mainly present as community-acquired pneumonia (CAP). However, most cases are treated without diagnostic testing, and the importance of\n                      <jats:italic>Chlamydia psittaci<\/jats:italic>\n                      infection as a cause of CAP is therefore unclear. Diagnostic tools, including culture, serologic test, and PCR-based methods, are available but prone to false negative results. Metagenomic next-generation sequencing (mNGS) has been increasingly used in the diagnosis of infectious diseases, particularly when conventional diagnostic approaches have limitation.\n                    <\/jats:p>\n                    <jats:p>\n                      Detection of nucleic acid sequence of\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      in respiratory tract samples by metagenomic next-generation sequencing (mNGS) is effective for early diagnosis of severe\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia. Timely treatment based on tetracycline can reduce unnecessary use of antibiotics and improve prognosis of patients with severe\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Clinical data of thirteen patients with severe\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia diagnosed by mNGS were collected. Clinical manifestations, treatment and prognosis of patients were summarized.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The typical symptoms of pneumonia caused by\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      include fever, headache, myalgia, cough, and dyspnea. In the current study, all patients met the criteria for severe\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia and received mechanical ventilation, including noninvasive mechanical ventilation (five\/thirteen) and invasive mechanical ventilation (eight\/thirteen). The findings showed that patients with\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia presented with normal or slightly increased leucocytes and procalcitonin, and high C-reactive protein levels. Computed tomography manifestations included consolidation of lung parenchyma, with air bronchogram and pleural effusion in some patients. mNGS analysis results were obtained within 48\u201372\u00a0h. Eleven patients fully recovered after targeted treatment, however, two patients died from secondary multidrug-resistant\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The findings of the current study show that mNGS is effective in diagnosis of\n                      <jats:italic>C. psittaci<\/jats:italic>\n                      pneumonia, and has significant diagnosis value in patients with severe infection. Patients responds well to the timely use of appropriate antibiotics.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12890-021-01673-6","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T13:04:58Z","timestamp":1632402298000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Application of metagenomic next-generation sequencing in the diagnosis of severe pneumonia caused by Chlamydia psittaci"],"prefix":"10.1186","volume":"21","author":[{"given":"Huan-huan","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan-fang","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang-yan","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"issue":"2","key":"1673_CR1","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.tvjl.2013.10.034","volume":"199","author":"ID Kalmar","year":"2014","unstructured":"Kalmar ID, Dicxk V, Dossche L, et al. 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