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Few candidate gene associations have been reported for AS and the current understanding of its pathogenesis remains still poor. Thus, the exact mechanism of AS is needed to urgently be disclosed. The purpose of this study was to identify candidate genes involving in AS disease.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods and results<\/jats:title>\n                <jats:p>GSE25101 publicly available microarray and GSE117769 RNA-seq datasets of AS patients were obtained for bioinformatics analyses. Gene set enrichment analysis showed that in the microarray dataset, the ribosome pathway was significantly up-regulated in AS compared with controls. Furthermore, some ribosomal components demonstrated overexpression in patients in the RNA-seq dataset. To confirm the findings, 20 AS patients and 20 matching controls were selected from the Rheumatology Research Center clinic, Shariati Hospital. PBMCs were separated from whole blood and RNA contents were extracted. Following the results of datasets analysis, the expression level of <jats:italic>rRNA5.8S<\/jats:italic> pseudogene, <jats:italic>rRNA18S<\/jats:italic> pseudogene, <jats:italic>RPL23<\/jats:italic>, <jats:italic>RPL7<\/jats:italic>, and <jats:italic>RPL17<\/jats:italic> genes were measured through real-time PCR. Our findings showed dysregulation of <jats:italic>rRNA5.8S<\/jats:italic> and <jats:italic>rRNA18S<\/jats:italic> pseudogenes, and also the <jats:italic>RPL17<\/jats:italic> gene in patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Considering that genes involved in ribosome biogenesis contributed to some AS-associated biological processes as well as diseases that have comorbidities with AS, our results might advance our understanding of the pathological mechanisms of ankylosing spondylitis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12891-021-04662-2","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T12:05:37Z","timestamp":1631621137000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dysregulation of ribosome-related genes in ankylosing spondylitis: a systems biology approach and experimental method"],"prefix":"10.1186","volume":"22","author":[{"given":"Arezou","family":"Lari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid Gholami","family":"Pourbadie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Sharifi-Zarchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Akhtari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila Nejatbakhsh","family":"Samimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmadreza","family":"Jamshidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-8831","authenticated-orcid":false,"given":"Mahdi","family":"Mahmoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,14]]},"reference":[{"key":"4662_CR1","doi-asserted-by":"crossref","unstructured":"Dibra D, Xia X, Gagea M, Lozano G, Li S. 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