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The role of pericytes in the pathogenesis of fibrosis has recently been determined. Extracellular vesicle (EV)\u2013mimetic nanovesicles (NVs) have attracted attention regarding intercellular communication between cells in the field of fibrosis. However, the global gene expression of pericyte-derived EV\u2013mimetic NVs (PC\u2013NVs) in regulating fibrosis remains unknown. Here, we used RNA-sequencing technology to investigate the potential target genes regulated by PC\u2013NVs in primary fibroblasts derived from human PD plaque.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human primary fibroblasts derived from normal and PD patients was cultured and treated with cavernosum pericytes isolated extracellular vesicle (EV)\u2013mimetic nanovesicles (NVs). A global gene expression RNA-sequencing assay was performed on normal fibroblasts, PD fibroblasts, and PD fibroblasts treated with PC\u2013NVs. Reverse transcription polymerase chain reaction (RT-PCR) was used for sequencing data validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 4135 genes showed significantly differential expression in the normal fibroblasts, PD fibroblasts, and PD fibroblasts treated with PC\u2013NVs. However, only 91 contra-regulated genes were detected among the three libraries. Furthermore, 20 contra-regulated genes were selected and 11 showed consistent changes in the RNA-sequencing assay, which were validated by RT-PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The gene expression profiling results suggested that these validated genes may be good targets for understanding potential mechanisms and conducting molecular studies into PD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12894-021-00872-x","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T04:03:01Z","timestamp":1628222581000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["RNA-sequencing profiling analysis of pericyte-derived extracellular vesicle\u2013mimetic nanovesicles-regulated genes in primary cultured fibroblasts from normal and Peyronie\u2019s disease penile tunica albuginea"],"prefix":"10.1186","volume":"21","author":[{"given":"Guo Nan","family":"Yin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Piao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyeon","family":"Ock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Hye","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do-Kyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Song","family":"Gho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Kyu","family":"Suh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0025-6025","authenticated-orcid":false,"given":"Ji-Kan","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,6]]},"reference":[{"key":"872_CR1","doi-asserted-by":"publisher","first-page":"1234","DOI":"10.1097\/01.ju.0000053800.62741.fe","volume":"169","author":"SS Gholami","year":"2003","unstructured":"Gholami SS, Gonzalez-Cadavid NF, Lin CS, Rajfer J, Lue TF. 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And all male C57BL\/6J mice (8\u00a0weeks old, Orient Bio, Korea) used in this study were approved by the Institutional Animal Care and Use Committee of our University (approval number: 171129-527), respectively. All authors of the manuscript have read and agreed to its content of the abstract.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All participating authors agree to publish in \u201c<i>BMC urology<\/i>\u201d.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"103"}}