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When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This study aimed to identify and characterize the<jats:italic>GmRD26<\/jats:italic>promoter (p<jats:italic>GmRD26<\/jats:italic>), which is involved in the regulation of plant responses to drought stress. The expression profile of the<jats:italic>GmRD26<\/jats:italic>gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that<jats:italic>GmRD26<\/jats:italic>is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the<jats:italic>GmRD26<\/jats:italic>promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on<jats:italic>A. thaliana<\/jats:italic>plants containing the complete construct of p<jats:italic>GmRD26<\/jats:italic>::<jats:italic>GUS<\/jats:italic>(2.054\u2009bp) and two promoter modules, p<jats:italic>GmRD26A<\/jats:italic>::<jats:italic>GUS<\/jats:italic>(909 pb) and p<jats:italic>GmRD26B<\/jats:italic>::<jats:italic>GUS<\/jats:italic>(435\u2009bp), controlling the expression of the \u03b2-glucuronidase (<jats:italic>uidA<\/jats:italic>) gene. Analysis of GUS activity has demonstrated that p<jats:italic>GmRD26<\/jats:italic>and p<jats:italic>GmRD26A<\/jats:italic>induce strong reporter gene expression, as the p<jats:italic>AtRD29<\/jats:italic>positive control promoter under ABA and PEG treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The full-length promoter p<jats:italic>GmRD26<\/jats:italic>and the p<jats:italic>GmRD26A<\/jats:italic>module provides an improved<jats:italic>uidA<\/jats:italic>transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that p<jats:italic>GmRD26A<\/jats:italic>may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12896-019-0561-3","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T08:01:34Z","timestamp":1574236894000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application"],"prefix":"10.1186","volume":"19","author":[{"given":"Elinea O.","family":"Freitas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno P.","family":"Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela T.","family":"Louren\u00e7o-Tessutti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabr\u00edcio B. 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