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The samples divided into a control and four sample groups (n\u2009=\u200920). All the samples were immersed in different solutions as follows, Control: Normal saline for 15\u00a0min, G1and G2: 2.5% NaOCl for 15\u00a0min, G3: 2.5% NaOCl for 15\u00a0min, followed by 5% STS for 10\u00a0min, G4: Normal saline for 15\u00a0min followed by 5% STS for 10\u00a0min. All groups except G1 incubated for one week before the test. The micro-hardness of samples was measured. Data were analyzed using the Kruskal\u2013Wallis test for pairwise comparisons. A\n                      <jats:italic>p<\/jats:italic>\n                      value &lt;\u20090.05 was considered significant\n                      <jats:bold>.<\/jats:bold>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All groups showed a significant decrease in the micro-hardness value compared with the control group. NaOCl for one week (G2) reduced the micro-hardness of dentine compared with samples, tested immediately after immersion in NaOCl (G1) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). NaOCl alone (G2) or treated with STS (G3) resulted in a significant decrease in micro-hardness compared with the STS group (G4) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>STS as a neutralizing agent could not prevent the dentin micro-hardness downturn caused by NaOCl.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12903-020-01320-2","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T09:03:10Z","timestamp":1605171790000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of sodium thiosulfate neutralizing effect on micro-hardness of dentin treated with sodium hypochlorite"],"prefix":"10.1186","volume":"20","author":[{"given":"Safoora","family":"Sahebi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Sobhnamayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3035-2926","authenticated-orcid":false,"given":"Fariborz","family":"Moazami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadhasan","family":"Naseri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"1320_CR1","doi-asserted-by":"crossref","unstructured":"Haikel Y, Gorce F, Allemann C, VOEGEL JC. 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