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Lymphatic vessels were quantified in intra and peritumoral areas by sequential analysis and hot spot evaluation. Associations of the ILVD and PLVD with clinicopathologic parameters were determined by Chi-square or Fisher\u2019s exact test. The 5 and 10-year survival rates were calculated by the Kaplan\u2013Meier and compared using the log-rank test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant association was observed between ILVD or PLDV and clinicopathologic variables including occult lymph node metastasis, or clinical follow-up. However, ILVD showed a significant association with regional recurrence (p\u2009=\u20090.040). The perineural invasion was associated with PLVD (p\u2009=\u20090.041). Disease-specific (p\u2009=\u20090.044) and disease-free survivals (p\u2009=\u20090.016) had significant association with PLVD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The intra or peritumoral lymphatic vessel density had no predictive value for occult lymph node metastasis in the early stages of oral cancer arising in the tongue or floor of mouth.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12903-021-01459-6","type":"journal-article","created":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T11:03:13Z","timestamp":1615460593000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Association of lymph vessel density with occult lymph node metastasis and prognosis in oral squamous cell carcinoma"],"prefix":"10.1186","volume":"21","author":[{"given":"Simone E. 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Camargo Cancer Center ethics committee (Protocol Number: 746\/05) and was performed according to the Helsinki declaration principles. The informed consent was waived by the A.C. Camargo Cancer Center ethics committee once it was a retrospective study that included the analysis of specimens that were collected previously for diagnostic and\/or treatment purposes.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"None to declare.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"114"}}