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However, <jats:italic>fimA<\/jats:italic> genotypes distribution of <jats:italic>Pg<\/jats:italic>, the origination of <jats:italic>Pg<\/jats:italic> in tissue, and its prognostic value are inconclusive. We aimed to investigate the frequency of <jats:italic>fimA<\/jats:italic> genotypes in OSCC patients, study the association between <jats:italic>Pg<\/jats:italic> and OSCC, and explore the prognostic value of <jats:italic>Pg<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The abundance of <jats:italic>Pg<\/jats:italic> in saliva from the OSCC group and the OSCC-free group was analysed by qPCR. The presence of <jats:italic>Pg<\/jats:italic> was explored in OSCC tissue and para-cancerous tissue by in situ hybridization. The frequency of <jats:italic>fimA<\/jats:italic> genotypes in saliva and OSCC tissue was determined by PCR, then PCR products were sequenced and compared. Clinical data were extracted, and patients followed up for a median period of 23\u00a0months. Clinicopathological variables were compared with the abundance of <jats:italic>Pg<\/jats:italic> using Pearson Chi-square test or Fisher\u2019s exact test. The disease-free survival (DFS) rate was calculated by Kaplan\u2013Meier method with log-rank tests<jats:bold>.<\/jats:bold><\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Comparing the OSCC-free group, 95 patients with OSCC showed a high abundance of <jats:italic>Pg<\/jats:italic> in saliva (<jats:italic>P<\/jats:italic>\u2009=\u20090.033), and OSCC tissue showed strong in situ expression of <jats:italic>Pg<\/jats:italic> compared with paired normal tissue. Patients with OSCC showed a dominant distribution of <jats:italic>Pg<\/jats:italic> with genotype I\u2009+\u2009Ib (21.1%), II (31.6%), and IV (21.1%). <jats:italic>FimA<\/jats:italic> genotypes detected in saliva were in accordance with those in OSCC tissue, there was, moreover, a significant similarity in amplified <jats:italic>Pg<\/jats:italic> fragments. Of the 94 responsive OSCC patients, the recurrence rate was 26.6% (25\/94). Overabundance of <jats:italic>Pg<\/jats:italic> in saliva showed advanced pathologic staging (<jats:italic>P<\/jats:italic>\u2009=\u20090.008), longer disease-free time (<jats:italic>P<\/jats:italic>\u2009=\u20090.029) and lower recurrence rate (<jats:italic>P<\/jats:italic>\u2009=\u20090.033). The overabundance of <jats:italic>Pg<\/jats:italic> in saliva was associated with improved disease-free survival (<jats:italic>P<\/jats:italic>\u2009=\u20090.049).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This study indicated that <jats:italic>Pg<\/jats:italic> might involve in the pathogenesis of OSCC, <jats:italic>Pg<\/jats:italic> carrying <jats:italic>fimA<\/jats:italic> I, Ib, II, and IV were prevalent genotypes in patients with OSCC, the provenance of <jats:italic>Pg<\/jats:italic> in OSCC tissue might be from the salivary microbial reservoir, and the abundance of <jats:italic>Pg<\/jats:italic> in saliva might consider as a favorable potential prognostic indicator in OSCC.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12903-021-01580-6","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T11:03:03Z","timestamp":1620039783000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Salivary Porphyromonas gingivalis predicts outcome in oral squamous cell carcinomas: a cohort study"],"prefix":"10.1186","volume":"21","author":[{"given":"Qingli","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erhui","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengjun","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,3]]},"reference":[{"issue":"6","key":"1580_CR1","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","volume":"68","author":"F Bray","year":"2018","unstructured":"Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. 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Informed consent was written by each patient. Animal Ethics clearance is not applicable, as this study does not involve any animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"228"}}