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This study aimed to assess the origin of MMP-8 and Lactoferrin in periodontitis patients and to identify the degree to which conventional clinical parameters correlate with their presence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of ten periodontitis and ten healthy patients were included in this study. Whole saliva (stimulated and unstimulated), parotid\/sublingual glandular fluid and gingival crevicular fluid from pockets and sulci were tested for MMP-8 and Lactoferrin and protein concentrations were quantified using an ELISA assay. Clinical parameters were checked for potential associations with MMP-8 and Lactoferrin levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Periodontal patients presented higher concentrations of MMP-8 and Lactoferrin in pockets than other sources (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.03). Lactoferrin measurement was higher in the parotid compared to sublingual glandular fluid in periodontitis patients (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.03). Increased probing pocket depth was positively correlated with high MMP-8 and Lactoferrin levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Periodontal pockets appear to be the major source of active matrix metalloproteinase and Lactoferrin, which also may also enter the oral cavity through the salivary glands. Since clinically healthy sites in periodontitis patients also had elevated biomarker levels, gingival crevicular fluid biomarker testing may be more predictive of future tissue breakdown than conventional clinical parameters.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12903-021-01743-5","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T05:03:15Z","timestamp":1628139795000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Origin of MMP-8 and Lactoferrin levels from gingival crevicular fluid, salivary glands and whole saliva"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7732-2118","authenticated-orcid":false,"given":"Liza L.","family":"Ramenzoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Hofer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Solderer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Wiedemeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Attin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Schmidlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"1743_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1902\/annals.1999.4.1.1","volume":"4","author":"GC Armitage","year":"1999","unstructured":"Armitage GC. 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The written informed consent including biomedical, clinical, and biometric data was obtained from all individual participants included in the study. The study was approved by the Canton of Zurich Ethics Committee (BASEC-Nr. 2016-00243).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The written informed consent for publication was obtained from all individual participants included in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"385"}}