{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T06:19:05Z","timestamp":1777184345810,"version":"3.51.4"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T00:00:00Z","timestamp":1640908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T00:00:00Z","timestamp":1640908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"This study was funded by the START funding program of the medical faculty of Aachen University.","award":["research project 105\/18"],"award-info":[{"award-number":["research project 105\/18"]}]},{"DOI":"10.13039\/501100007210","name":"RWTH Aachen University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007210","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Oral Health"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>The aim of the present study was to assess the development of bacterial deposits and morphological parameters around dental zirconia and titanium implants compared with natural teeth during systemic bisphosphonate medication.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Materials and methods<\/jats:title>\n                <jats:p>Fifty-four rats were randomly allocated into one control group and two experimental groups (drug application of zoledronic and alendronic acid), with 18 animals in each group. After 4\u00a0weeks of drug delivery, either a zirconia or a titanium implant was immediately inserted. Microbiological analysis conducted 1\u00a0week, 8\u00a0weeks, and 12\u00a0weeks after surgery included total bacterial count and composition measurements. Samples were analyzed in a scanning electron microscope (SEM) equipped with energy-dispersive X-ray spectroscopy (EDX). Bone cell morphology was analyzed by transmission electron microscopy (TEM).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>One week after surgery, titanium and zirconia implants of the alendronic acid and control group showed a significantly higher bacterial count when compared to natural teeth in rats with zoledronic acid administration (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Less significant differences were recorded after 3\u00a0months, at which time no inter-material differences were evaluated (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). I\nn the control group, TEM analysis showed that the osteoblasts had a strongly developed endoplasmic reticulum. In contrast, the endoplasmic reticulum of the osteoblasts in drug-treated animals was significantly less developed, indicating less activity.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Within the limits of this study, neither implant material was superior to the other at 3-month follow-up. With regard to the treatment and complications of patients with bisphosphonates, the implant material should not be an influencing factor. Bisphosphonates can be used in the rat model to reduce not only the activity of osteoclasts but also osteoblasts of the peri-implant bone.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12903-021-02031-y","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T18:02:33Z","timestamp":1640973753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In vivo and in vitro analysis in a rat model using zoledronate and alendronate medication: microbiological and scanning electron microscopy findings on peri-implant rat tissue"],"prefix":"10.1186","volume":"21","author":[{"given":"Kristian","family":"Kniha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva Miriam","family":"Buhl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan Christian","family":"M\u00f6hlhenrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"H\u00f6lzle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elmar","family":"Hellwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Al-Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Modabber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,12,31]]},"reference":[{"issue":"5 Suppl","key":"2031_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.joms.2008.10.005","volume":"67","author":"SL Ruggiero","year":"2009","unstructured":"Ruggiero SL. Bisphosphonate-related osteonecrosis of the jaw (BRONJ): initial discovery and subsequent development. J Oral Maxillofac Surg. 2009;67(5 Suppl):13\u20138.","journal-title":"J Oral Maxillofac Surg"},{"issue":"1","key":"2031_CR2","first-page":"2","volume":"7","author":"MD Francis","year":"2007","unstructured":"Francis MD, Valent DJ. Historical perspectives on the clinical development of bisphosphonates in the treatment of bone diseases. J Musculoskelet Neuronal Interact. 2007;7(1):2\u20138.","journal-title":"J Musculoskelet Neuronal Interact"},{"key":"2031_CR3","unstructured":"Health CAfDaTi: CADTH rapid response reports. In: Denosumab versus zoledronic acid for men with osteoporosis: a review of clinical effectiveness and guidelines. Canadian Agency for Drugs and Technologies in Health; 2016."},{"issue":"11","key":"2031_CR4","doi-asserted-by":"publisher","first-page":"963","DOI":"10.2165\/0002512-200825110-00007","volume":"25","author":"ED Deeks","year":"2008","unstructured":"Deeks ED, Perry CM. Zoledronic acid: a review of its use in the treatment of osteoporosis. Drugs Aging. 2008;25(11):963\u201386.","journal-title":"Drugs Aging"},{"issue":"17","key":"2031_CR5","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.1007\/s40265-016-0662-4","volume":"76","author":"S Dhillon","year":"2016","unstructured":"Dhillon S. Zoledronic acid (Reclast((R)), Aclasta((R))): a review in osteoporosis. Drugs. 2016;76(17):1683\u201397.","journal-title":"Drugs"},{"key":"2031_CR6","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1196\/annals.1346.041","volume":"1068","author":"RG Russell","year":"2006","unstructured":"Russell RG. Bisphosphonates: from bench to bedside. Ann N Y Acad Sci. 2006;1068:367\u2013401.","journal-title":"Ann N Y Acad Sci"},{"issue":"4","key":"2031_CR7","doi-asserted-by":"publisher","first-page":"241","DOI":"10.2165\/00024677-200201040-00006","volume":"1","author":"JP Devogelaer","year":"2002","unstructured":"Devogelaer JP. Modern therapy for Paget\u2019s disease of bone: focus on bisphosphonates. Treat Endocrinol. 2002;1(4):241\u201357.","journal-title":"Treat Endocrinol"},{"issue":"1","key":"2031_CR8","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.bone.2011.04.022","volume":"49","author":"RG Russell","year":"2011","unstructured":"Russell RG. Bisphosphonates: the first 40 years. Bone. 2011;49(1):2\u201319.","journal-title":"Bone"},{"issue":"9","key":"2031_CR9","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1016\/j.jcms.2017.05.028","volume":"45","author":"S Aljohani","year":"2017","unstructured":"Aljohani S, Fliefel R, Ihbe J, Kuhnisch J, Ehrenfeld M, Otto S. What is the effect of anti-resorptive drugs (ARDs) on the development of medication-related osteonecrosis of the jaw (MRONJ) in osteoporosis patients: a systematic review. J Craniomaxillofac Surg. 2017;45(9):1493\u2013502.","journal-title":"J Craniomaxillofac Surg"},{"issue":"2","key":"2031_CR10","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1111\/j.1600-0501.2011.02220.x","volume":"23","author":"GE Salvi","year":"2012","unstructured":"Salvi GE, Aglietta M, Eick S, Sculean A, Lang NP, Ramseier CA. Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans. Clin Oral Implant Res. 2012;23(2):182\u201390.","journal-title":"Clin Oral Implant Res"},{"issue":"4","key":"2031_CR11","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1016\/j.ijom.2018.10.017","volume":"48","author":"K Clever","year":"2019","unstructured":"Clever K, Schlegel KA, Kniha H, Conrads G, Rink L, Modabber A, Holzle F, Kniha K. Experimental peri-implant mucositis around titanium and zirconia implants in comparison to a natural tooth: part 2-clinical and microbiological parameters. Int J Oral Maxillofac Surg. 2019;48(4):560\u20135.","journal-title":"Int J Oral Maxillofac Surg"},{"key":"2031_CR12","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1186\/s12967-015-0568-z","volume":"13","author":"S Kalyan","year":"2015","unstructured":"Kalyan S, Wang J, Quabius ES, Huck J, Wiltfang J, Baines JF, Kabelitz D. Systemic immunity shapes the oral microbiome and susceptibility to bisphosphonate-associated osteonecrosis of the jaw. J Transl Med. 2015;13:212.","journal-title":"J Transl Med"},{"issue":"10","key":"2031_CR13","doi-asserted-by":"publisher","first-page":"1937.e1931","DOI":"10.1016\/j.joms.2014.04.037","volume":"72","author":"D Holzinger","year":"2014","unstructured":"Holzinger D, Seemann R, Matoni N, Ewers R, Millesi W, Wutzl A. Effect of dental implants on bisphosphonate-related osteonecrosis of the jaws. J Oral Maxillofac Surg. 2014;72(10):1937.e1931-1938.","journal-title":"J Oral Maxillofac Surg"},{"issue":"1","key":"2031_CR14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/s00784-012-0708-2","volume":"17","author":"C Jacobsen","year":"2013","unstructured":"Jacobsen C, Metzler P, Rossle M, Obwegeser J, Zemann W, Gratz KW. Osteopathology induced by bisphosphonates and dental implants: clinical observations. Clin Oral Investig. 2013;17(1):167\u201375.","journal-title":"Clin Oral Investig"},{"issue":"5","key":"2031_CR15","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1111\/clr.12088","volume":"25","author":"TG Kwon","year":"2014","unstructured":"Kwon TG, Lee CO, Park JW, Choi SY, Rijal G, Shin HI. Osteonecrosis associated with dental implants in patients undergoing bisphosphonate treatment. Clin Oral Implant Res. 2014;25(5):632\u201340.","journal-title":"Clin Oral Implant Res"},{"issue":"8","key":"2031_CR16","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1016\/j.bjoms.2013.06.011","volume":"51","author":"JL Lopez-Cedrun","year":"2013","unstructured":"Lopez-Cedrun JL, Sanroman JF, Garcia A, Penarrocha M, Feijoo JF, Limeres J, Diz P. Oral bisphosphonate-related osteonecrosis of the jaws in dental implant patients: a case series. Br J Oral Maxillofac Surg. 2013;51(8):874\u20139.","journal-title":"Br J Oral Maxillofac Surg"},{"issue":"5","key":"2031_CR17","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1111\/cid.12048","volume":"16","author":"Y Tam","year":"2014","unstructured":"Tam Y, Kar K, Nowzari H, Cha HS, Ahn KM. Osteonecrosis of the jaw after implant surgery in patients treated with bisphosphonates\u2014a presentation of six consecutive cases. Clin Implant Dent Relat Res. 2014;16(5):751\u201361.","journal-title":"Clin Implant Dent Relat Res"},{"issue":"1","key":"2031_CR18","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.bone.2011.03.774","volume":"49","author":"FH Ebetino","year":"2011","unstructured":"Ebetino FH, Hogan AM, Sun S, Tsoumpra MK, Duan X, Triffitt JT, Kwaasi AA, Dunford JE, Barnett BL, Oppermann U, et al. The relationship between the chemistry and biological activity of the bisphosphonates. Bone. 2011;49(1):20\u201333.","journal-title":"Bone"},{"key":"2031_CR19","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1186\/s12866-014-0312-5","volume":"14","author":"AC Anderson","year":"2014","unstructured":"Anderson AC, Sanunu M, Schneider C, Clad A, Karygianni L, Hellwig E, Al-Ahmad A. Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing. BMC Microbiol. 2014;14:312\u2013312.","journal-title":"BMC Microbiol"},{"issue":"3","key":"2031_CR20","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1099\/jmm.0.000682","volume":"67","author":"A Al-Ahmad","year":"2018","unstructured":"Al-Ahmad A, Muzafferiy F, Anderson AC, W\u00f6lber JP, Ratka-Kr\u00fcger P, Fretwurst T, Nelson K, Vach K, Hellwig E. Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning. J Med Microbiol. 2018;67(3):332\u201340.","journal-title":"J Med Microbiol"},{"issue":"3","key":"2031_CR21","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/j.dental.2020.01.013","volume":"36","author":"X He","year":"2020","unstructured":"He X, Reichl FX, Milz S, Michalke B, Wu X, Sprecher CM, Yang Y, Gahlert M, R\u00f6hling S, Kniha H, et al. Titanium and zirconium release from titanium- and zirconia implants in mini pig maxillae and their toxicity in vitro. Dent Mater. 2020;36(3):402\u201312.","journal-title":"Dent Mater"},{"issue":"9","key":"2031_CR22","doi-asserted-by":"publisher","first-page":"2195","DOI":"10.1007\/s10856-013-4978-2","volume":"24","author":"CM Sprecher","year":"2013","unstructured":"Sprecher CM, Gahlert M, Rohling S, Kniha H, Gueorguiev B, Milz S. Comparison of imaging methods used for dental implant osseous integration assessment. J Mater Sci Mater Med. 2013;24(9):2195\u2013200.","journal-title":"J Mater Sci Mater Med"},{"issue":"4","key":"2031_CR23","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1111\/j.1708-8208.2009.00168.x","volume":"12","author":"M Gahlert","year":"2010","unstructured":"Gahlert M, Rohling S, Wieland M, Eichhorn S, Kuchenhoff H, Kniha H. A comparison study of the osseointegration of zirconia and titanium dental implants. A biomechanical evaluation in the maxilla of pigs. Clin Implant Dent Relat Res. 2010;12(4):297\u2013305.","journal-title":"Clin Implant Dent Relat Res"},{"issue":"19","key":"2031_CR24","doi-asserted-by":"publisher","first-page":"4349","DOI":"10.3390\/ma13194349","volume":"13","author":"A Ibrahim","year":"2020","unstructured":"Ibrahim A, Heitzer M, Bock A, Peters F, M\u00f6hlhenrich SC, H\u00f6lzle F, Modabber A, Kniha K. Relationship between implant geometry and primary stability in different bony defects and variant bone densities: an in vitro study. Materials. 2020;13(19):4349.","journal-title":"Materials"},{"issue":"11","key":"2031_CR25","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1016\/j.ijom.2020.04.003","volume":"49","author":"K Kniha","year":"2020","unstructured":"Kniha K, Bock A, Peters F, Heitzer M, Modabber A, Kniha H, H\u00f6lzle F, M\u00f6hlhenrich SC. Aesthetic aspects of adjacent maxillary single-crown implants-influence of zirconia and titanium as implant materials. Int J Oral Maxillofac Surg. 2020;49(11):1489\u201396.","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"2","key":"2031_CR26","doi-asserted-by":"publisher","first-page":"e694","DOI":"10.1111\/jopr.12766","volume":"28","author":"K Kniha","year":"2019","unstructured":"Kniha K, Schlegel KA, Kniha H, Modabber A, Neukam F, Kniha K. Papilla-crown height dimensions around zirconium dioxide implants in the esthetic area: a 3-year follow-up study. J Prosthodont. 2019;28(2):e694\u20138.","journal-title":"J Prosthodont"},{"key":"2031_CR27","doi-asserted-by":"publisher","first-page":"e195","DOI":"10.11607\/prd.3282","volume":"39","author":"K Kniha","year":"2018","unstructured":"Kniha K, Kniha H, Grunert I, Edelhoff D, Holzle F, Modabber A. Esthetic evaluation of maxillary single-tooth zirconia implants in the esthetic zone. Int J Periodontics Restorative Dent. 2018;39:e195\u2013201.","journal-title":"Int J Periodontics Restorative Dent"},{"issue":"1","key":"2031_CR28","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1186\/s12903-018-0636-x","volume":"18","author":"KH Bormann","year":"2018","unstructured":"Bormann KH, Gellrich NC, Kniha H, Schild S, Weingart D, Gahlert M. A prospective clinical study to evaluate the performance of zirconium dioxide dental implants in single-tooth edentulous area: 3-year follow-up. BMC Oral Health. 2018;18(1):181.","journal-title":"BMC Oral Health"},{"issue":"1","key":"2031_CR29","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.bjoms.2017.11.005","volume":"56","author":"K Kniha","year":"2018","unstructured":"Kniha K, Modabber A, Kniha H, Mohlhenrich SC, Holzle F, Milz S. Dimensions of hard and soft tissue around adjacent, compared with single-tooth, zirconia implants. Br J Oral Maxillofac Surg. 2018;56(1):43\u20137.","journal-title":"Br J Oral Maxillofac Surg"},{"issue":"4","key":"2031_CR30","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.ijom.2017.10.013","volume":"47","author":"K Kniha","year":"2018","unstructured":"Kniha K, Schlegel KA, Kniha H, Modabber A, Holzle F, Kniha K. Evaluation of peri-implant bone levels and soft tissue dimensions around zirconia implants-a three-year follow-up study. Int J Oral Maxillofac Surg. 2018;47(4):492\u20138.","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"3","key":"2031_CR31","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1177\/0022034512472335","volume":"92","author":"J Abtahi","year":"2013","unstructured":"Abtahi J, Agholme F, Sandberg O, Aspenberg P. Effect of local vs. systemic bisphosphonate delivery on dental implant fixation in a model of osteonecrosis of the jaw. J Dent Res. 2013;92(3):279\u201383.","journal-title":"J Dent Res"},{"issue":"6","key":"2031_CR32","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1016\/j.injury.2015.02.011","volume":"46","author":"M Bernhardsson","year":"2015","unstructured":"Bernhardsson M, Sandberg O, Aspenberg P. Anti-RANKL treatment improves screw fixation in cancellous bone in rats. Injury. 2015;46(6):990\u20135.","journal-title":"Injury"},{"key":"2031_CR33","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s00784-020-03361-x","volume":"25","author":"K Kniha","year":"2020","unstructured":"Kniha K, Buhl EM, Hermanns-Sachweh B, Al-Sibai F, Bock A, Peters F, H\u00f6lzle F, Modabber A. Implant removal using thermal necrosis-an in vitro pilot study. Clin Oral Investig. 2020;25:265\u201373.","journal-title":"Clin Oral Investig"},{"issue":"4","key":"2031_CR34","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.3758\/BRM.41.4.1149","volume":"41","author":"F Faul","year":"2009","unstructured":"Faul F, Erdfelder E, Buchner A, Lang AG. Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses. Behav Res Methods. 2009;41(4):1149\u201360.","journal-title":"Behav Res Methods"},{"issue":"1","key":"2031_CR35","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1128\/MMBR.62.1.71-109.1998","volume":"62","author":"H Marcotte","year":"1998","unstructured":"Marcotte H, Lavoie MC. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev. 1998;62(1):71\u2013109.","journal-title":"Microbiol Mol Biol Rev"},{"issue":"3","key":"2031_CR36","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.archoralbio.2012.07.017","volume":"58","author":"Z Jabbour","year":"2013","unstructured":"Jabbour Z, do Nascimento C, Kotake BG, El-Hakim M, Henderson JE, de Albuquerque Jr RF. Assessing the oral microbiota of healthy and alcohol-treated rats using whole-genome DNA probes from human bacteria. Arch Oral Biol. 2013;58(3):317\u201323.","journal-title":"Arch Oral Biol"},{"issue":"9","key":"2031_CR37","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1016\/j.ijom.2015.12.017","volume":"45","author":"Z Jabbour","year":"2016","unstructured":"Jabbour Z, de Nascimento C, El-Hakim M, Henderson JE, de Albuquerque Jr RF. Bacterial profile and bone healing in rats receiving cancer therapeutic doses of bisphosphonates and corticosteroids: a pilot study. Int J Oral Maxillofac Surg. 2016;45(9):1162\u20139.","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"3","key":"2031_CR38","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1111\/clr.13556","volume":"31","author":"J Sun","year":"2020","unstructured":"Sun J, Eberhard J, Glage S, Held N, Voigt H, Schwabe K, Winkel A, Stiesch M. Development of a peri-implantitis model in the rat. Clin Oral Implant Res. 2020;31(3):203\u201314.","journal-title":"Clin Oral Implant Res"},{"issue":"11","key":"2031_CR39","doi-asserted-by":"publisher","first-page":"e49576","DOI":"10.1371\/journal.pone.0049576","volume":"7","author":"AC Anderson","year":"2012","unstructured":"Anderson AC, Hellwig E, Vespermann R, Wittmer A, Schmid M, Karygianni L, Al-Ahmad A. Comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. PLoS ONE. 2012;7(11):e49576.","journal-title":"PLoS ONE"},{"issue":"1","key":"2031_CR40","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1210\/jc.2009-1740","volume":"95","author":"S Khosla","year":"2010","unstructured":"Khosla S. Update in male osteoporosis. J Clin Endocrinol Metab. 2010;95(1):3\u201310.","journal-title":"J Clin Endocrinol Metab"},{"issue":"21","key":"2031_CR41","doi-asserted-by":"publisher","first-page":"10126","DOI":"10.1111\/jcmm.16949","volume":"25","author":"Q Xu","year":"2021","unstructured":"Xu Q, Zhan P, Li X, Mo F, Xu H, Liu Y, Lai Q, Zhang B, Dai M, Liu X. Bisphosphonate-enoxacin inhibit osteoclast formation and function by abrogating RANKL-induced JNK signalling pathways during osteoporosis treatment. J Cell Mol Med. 2021;25(21):10126\u201339.","journal-title":"J Cell Mol Med"},{"key":"2031_CR42","doi-asserted-by":"publisher","first-page":"105291","DOI":"10.1016\/j.archoralbio.2021.105291","volume":"132","author":"PHC Isaias","year":"2021","unstructured":"Isaias PHC, Silva PGB, do Nascimento IV, Verde M, Moreira MDS, Alves A, Sousa FB, Pereira KMA, Mota MRL. Effect of continuous and intermittent sodium alendronate oral dosing on post-extraction alveoli healing in rats. Arch Oral Biol. 2021;132:105291.","journal-title":"Arch Oral Biol"},{"issue":"1","key":"2031_CR43","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1186\/s12967-017-1230-8","volume":"15","author":"C Gross","year":"2017","unstructured":"Gross C, Weber M, Creutzburg K, M\u00f6bius P, Preidl R, Amann K, Wehrhan F. Osteoclast profile of medication-related osteonecrosis of the jaw secondary to bisphosphonate therapy: a comparison with osteoradionecrosis and osteomyelitis. J Transl Med. 2017;15(1):128.","journal-title":"J Transl Med"},{"issue":"1","key":"2031_CR44","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1056\/NEJMoa0802633","volume":"360","author":"RS Weinstein","year":"2009","unstructured":"Weinstein RS, Roberson PK, Manolagas SC. Giant osteoclast formation and long-term oral bisphosphonate therapy. N Engl J Med. 2009;360(1):53\u201362.","journal-title":"N Engl J Med"},{"issue":"3","key":"2031_CR45","doi-asserted-by":"publisher","first-page":"e421","DOI":"10.4317\/medoral.18449","volume":"18","author":"F Camacho-Alonso","year":"2013","unstructured":"Camacho-Alonso F, L\u00f3pez-Jornet P, Vicente-Hern\u00e1ndez A. Short-term effect of zoledronic acid upon fracture resistance of the mandibular condyle and femoral head in an animal model. Med Oral Patol Oral Cir Bucal. 2013;18(3):e421-426.","journal-title":"Med Oral Patol Oral Cir Bucal"},{"issue":"1","key":"2031_CR46","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1111\/odi.12212","volume":"21","author":"H Okata","year":"2015","unstructured":"Okata H, Nakamura M, Henmi A, Yamaguchi S, Mikami Y, Shimauchi H, Sasano Y. Calcification during bone healing in a standardised rat calvarial defect assessed by micro-CT and SEM-EDX. Oral Dis. 2015;21(1):74\u201382.","journal-title":"Oral Dis"},{"issue":"2","key":"2031_CR47","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s10867-011-9247-3","volume":"38","author":"N Kourkoumelis","year":"2012","unstructured":"Kourkoumelis N, Balatsoukas I, Tzaphlidou M. Ca\/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy. J Biol Phys. 2012;38(2):279\u201391.","journal-title":"J Biol Phys"}],"container-title":["BMC Oral Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-021-02031-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12903-021-02031-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-021-02031-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T09:04:00Z","timestamp":1666861440000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcoralhealth.biomedcentral.com\/articles\/10.1186\/s12903-021-02031-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,31]]},"references-count":47,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["2031"],"URL":"https:\/\/doi.org\/10.1186\/s12903-021-02031-y","relation":{},"ISSN":["1472-6831"],"issn-type":[{"value":"1472-6831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,31]]},"assertion":[{"value":"1 October 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 December 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 December 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"We confirm that all experiments were performed in accordance with relevant named guidelines and regulations that the authors complied with the ARRIVE guidelines. The study protocol was approved by the local ethics commission for animal studies. For this type of study, formal consent is not required.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not required.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"672"}}