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This study aimed to add molecular insight into internal root resorption. The present study was conducted to investigate the effect of hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) as an inducer of oxidative stress on the calcification ability of human dental pulp cells (hDPCs) and the involvement of inositol 1, 4, 5-trisphosphate (IP3).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material and methods<\/jats:title>\n                <jats:p>hDPCs (Lonza, Basel, Switzerland) were exposed to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Cell viability and reactive oxygen species (ROS) production were then evaluated. To investigate the effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> on the calcification ability of hDPCs, real-time PCR for alkaline phosphatase (ALP) mRNA expression, ALP staining, and Alizarin red staining were performed. Data were compared with those of hDPCs pretreated with 2-aminoethyldiphenylborate (2-APB), which is an IP3 receptor inhibitor.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> at concentrations above 250\u00a0\u00b5M significantly reduced cell viability (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). More ROS production occurred in 100\u00a0\u00b5M H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-treated hDPCs than in control cells (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). 2-APB significantly decreased the production (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-treated hDPCs showed significant reductions in ALP mRNA expression (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), ALP activity (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), and mineralized nodule deposition compared with negative control cells (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). 2-APB significantly inhibited these reductions (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01, respectively). Data are representative of three independent experiments with three replicates for each treatment and values are expressed as means\u2009\u00b1\u2009SD.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>To the best of our knowledge, this is the first study documenting the involvement of IP<jats:sub>3<\/jats:sub> signaling in the calcification ability of human dental pulp cells impaired by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12903-022-02467-w","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T18:44:02Z","timestamp":1664822642000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Oxidative stress impairs the calcification ability of human dental pulp cells"],"prefix":"10.1186","volume":"22","author":[{"given":"Satomi","family":"Shirawachi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Naruse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Takahasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Shindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Shiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"2467_CR1","doi-asserted-by":"publisher","DOI":"10.1136\/bcr-2013-201570","author":"P Gayathri","year":"2014","unstructured":"Gayathri P, Pandey RK, Jain E. 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