{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,3]],"date-time":"2024-03-03T00:12:06Z","timestamp":1709424726889},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T00:00:00Z","timestamp":1709337600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T00:00:00Z","timestamp":1709337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Faculty of Dentistry, Universiti Kebangsaan Malaysia","award":["DD-2021-005","DD-2021-005","DD-2021-005","DD-2021-005","DD-2021-005"],"award-info":[{"award-number":["DD-2021-005","DD-2021-005","DD-2021-005","DD-2021-005","DD-2021-005"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Oral Health"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Objectives<\/jats:title>\n                <jats:p>To determine the efficacy of a newly developed kit in dentine sialophosphoprotein (DSPP) detection and compare it with enzyme-linked immunosorbent assay (ELISA). User acceptance was also determined.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Materials and methods<\/jats:title>\n                <jats:p>This cross-sectional study consisted of 45 subjects who were divided into 3 groups based on the severity of root resorption using radiographs: normal (RO), mild (RM), and severe (RS). DSPP in GCF samples was analyzed using both methods. Questionnaires were distributed to 30 orthodontists to evaluate future user acceptance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The sensitivity and specificity of the kit were 0.98 and 0.8 respectively. The DSPP concentrations measured using ELISA were the highest in the RS group (6.33\u2009\u00b1\u20090.85 ng\/mL) followed by RM group (3.77\u2009\u00b1\u20090.36 ng\/mL) and the RO group had the lowest concentration (2.23\u2009\u00b1\u20090.55 ng\/mL). The new kit portrayed similar results as the ELISA, the optical density (OD) values were the highest in the RS group (0.62\u2009\u00b1\u20090.10) followed by RM group (0.33\u2009\u00b1\u20090.03) and the RO group (0.19\u2009\u00b1\u20090.06). The differences among all the groups were statistically significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) for both methods. The Pearson correlation coefficient showed a statistically significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) strong and positive correlation between DSPP concentrations and OD values.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The new kit was validated to detect the colour intensities of different severity of root resorptions. Most of the responses to the survey were positive towards the new kit for being a safer and simpler method to detect apical root resorption.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12903-024-04056-5","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T14:02:01Z","timestamp":1709388121000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A newly developed kit for dental apical root resorption detection: efficacy and acceptability"],"prefix":"10.1186","volume":"24","author":[{"given":"Jun Hong Steven","family":"Tan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farinawati","family":"Yazid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurfathiha Abu","family":"Kasim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahrul Hisham Zainal","family":"Ariffin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohaya Megat Abdul","family":"Wahab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,2]]},"reference":[{"issue":"1","key":"4056_CR1","doi-asserted-by":"publisher","first-page":"101","DOI":"10.21307\/aoj-2020-012","volume":"36","author":"Y Farinawati","year":"2020","unstructured":"Farinawati Y, Teh Y, Asma A, Shahrul Hisham ZA, Rohaya MAW. Detection methods of orthodontically induced inflammatory root resorption (OIIRR): a review. Australasian Orthodontic J. 2020;36(1):101\u20137.","journal-title":"Australasian Orthodontic J"},{"issue":"4","key":"4056_CR2","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1093\/ejo\/cjaa054","volume":"43","author":"SA Bellini-Pereira","year":"2020","unstructured":"Bellini-Pereira SA, Almeida J, Aliaga-Del Castillo A, Dos Santos CCO, Henriques JFC, Janson G. Evaluation of root resorption following orthodontic intrusion: a systematic review and meta-analysis. Eur J Orthod. 2020;43(4):432\u201341.","journal-title":"Eur J Orthod"},{"issue":"5","key":"4056_CR3","doi-asserted-by":"publisher","first-page":"329","DOI":"10.4041\/kjod.2021.51.5.329","volume":"51","author":"KC Toyokawa-Sperandio","year":"2021","unstructured":"Toyokawa-Sperandio KC, Conti ACCF, Fernandes TMF, Almeida-Pedrin RRd A, MRd, Oltramari PVP. External apical root resorption 6 months after initiation of orthodontic treatment: a randomized clinical trial comparing fixed appliances and orthodontic aligners. Korean J Orthod. 2021;51(5):329\u201336.","journal-title":"Korean J Orthod"},{"issue":"3","key":"4056_CR4","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.archoralbio.2006.08.018","volume":"52","author":"L Balducci","year":"2007","unstructured":"Balducci L, Ramachandran A, Hao J, Narayanan K, Evans C, George A. Biological markers for evaluation of root resorption. Arch Oral Biol. 2007;52(3):203\u20138.","journal-title":"Arch Oral Biol"},{"issue":"5","key":"4056_CR5","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1007\/s00056-020-00267-x","volume":"82","author":"KAA Mandour","year":"2020","unstructured":"Mandour KAA, Tawfeek MA, Montasser MA. Expression of biological markers in gingival crevicular fluid of teeth with orthodontically induced root resorption. J Orofac Orthop \/ Fortschr Der Kieferorthop\u00e4die. 2020;82(5):313\u201320.","journal-title":"J Orofac Orthop \/ Fortschr Der Kieferorthop\u00e4die"},{"issue":"1","key":"4056_CR6","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1186\/s12903-022-02178-2","volume":"22","author":"MN Mohd Zain","year":"2022","unstructured":"Mohd Zain MN, Md Yusof Z, Basri KN, Yazid F, Teh YX, Ashari A, et al. Multivariate versus univariate spectrum analysis of dentine sialophosphoprotein (DSPP) for root resorption prediction: a clinical trial. BMC Oral Health. 2022;22(1):151.","journal-title":"BMC Oral Health"},{"issue":"3","key":"4056_CR7","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1093\/ejo\/cjn024","volume":"30","author":"S Kereshanan","year":"2008","unstructured":"Kereshanan S, Stephenson P, Waddington R. Identification of dentine sialoprotein in gingival crevicular fluid during physiological root resorption and orthodontic tooth movement. Eur J Orthod. 2008;30(3):307\u201314.","journal-title":"Eur J Orthod"},{"issue":"1","key":"4056_CR8","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1093\/ejo\/26.1.25","volume":"26","author":"J Mah","year":"2004","unstructured":"Mah J, Prasad N. Dentine phosphoproteins in gingival crevicular fluid during root resorption. Eur J Orthod. 2004;26(1):25\u201330.","journal-title":"Eur J Orthod"},{"issue":"5","key":"4056_CR9","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/S0934-8832(11)80030-2","volume":"12","author":"WT Butler","year":"1992","unstructured":"Butler WT, Bhown M, Brunn JC, D\u2019Souza RN, Farach-Carson MC, Happonen RP, et al. Isolation, characterization and immunolocalization of a 53-kDal dentin sialoprotein (DSP). Matrix. 1992;12(5):343\u201351.","journal-title":"Matrix"},{"issue":"6","key":"4056_CR10","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1002\/jbmr.5650090609","volume":"9","author":"AL Bronckers","year":"1994","unstructured":"Bronckers AL, Farach-Carson MC, Van Waveren E, Butler WT. Immunolocalization of osteopontin, osteocalcin, and dentin sialoprotein during dental root formation and early cementogenesis in the rat. J Bone Miner Res. 1994;9(6):833\u201341.","journal-title":"J Bone Miner Res"},{"issue":"1","key":"4056_CR11","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.ajodo.2013.09.008","volume":"145","author":"H Sha","year":"2014","unstructured":"Sha H, Bai Y, Li S, Wang X, Yin Y. Comparison between electrochemical ELISA and spectrophotometric ELISA for the detection of dentine sialophosphoprotein for root resorption. Am J Orthod Dentofac Orthop. 2014;145(1):36\u201340.","journal-title":"Am J Orthod Dentofac Orthop"},{"issue":"1","key":"4056_CR12","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1007\/s11418-017-1144-z","volume":"72","author":"S Sakamoto","year":"2018","unstructured":"Sakamoto S, Putalun W, Vimolmangkang S, Phoolcharoen W, Shoyama Y, Tanaka H, et al. Enzyme-linked immunosorbent assay for the quantitative\/qualitative analysis of plant secondary metabolites. J Nat Med. 2018;72(1):32\u201342.","journal-title":"J Nat Med"},{"issue":"5","key":"4056_CR13","doi-asserted-by":"publisher","first-page":"e0177592","DOI":"10.1371\/journal.pone.0177592","volume":"12","author":"MM Billingsley","year":"2017","unstructured":"Billingsley MM, Riley RS, Day ES. Antibody-nanoparticle conjugates to enhance the sensitivity of ELISA-based detection methods. PLoS ONE. 2017;12(5):e0177592.","journal-title":"PLoS ONE"},{"issue":"1","key":"4056_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1021\/acsanm.9b02003","volume":"3","author":"Y Gao","year":"2020","unstructured":"Gao Y, Zhou Y, Chandrawati R. Metal and metal oxide nanoparticles to enhance the performance of enzyme-linked immunosorbent assay (ELISA). ACS Appl Nano Mater. 2020;3(1):1\u201321.","journal-title":"ACS Appl Nano Mater"},{"issue":"7","key":"4056_CR15","doi-asserted-by":"publisher","first-page":"2935","DOI":"10.1039\/C4NJ00253A","volume":"38","author":"L Zhan","year":"2014","unstructured":"Zhan L, Wu W, Yang X, Huang C. Gold nanoparticle-based enhanced ELISA for respiratory syncytial virus. New J Chem. 2014;38(7):2935\u201340.","journal-title":"New J Chem"},{"issue":"1","key":"4056_CR16","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1093\/ejo\/10.1.30","volume":"10","author":"E Levander","year":"1988","unstructured":"Levander E, Malmgren O. Evaluation of the risk of root resorption during orthodontic treatment: a study of upper incisors. Eur J Orthod. 1988;10(1):30\u20138.","journal-title":"Eur J Orthod"},{"issue":"2","key":"4056_CR17","doi-asserted-by":"publisher","first-page":"237","DOI":"10.21315\/aos2022.1702.OA07","volume":"17","author":"S Ahmad","year":"2022","unstructured":"Ahmad S, Noor Ayuni AS, Rohaya MAW. Quantitative assessment of dentine sialophosphoprotein, aspartate aminotransferase and lactate dehydrogenase in gingival crevicular fluid of teeth with root resorption. Archives Orofac Sci. 2022;17(2):237\u201346.","journal-title":"Archives Orofac Sci"},{"issue":"6","key":"4056_CR18","doi-asserted-by":"publisher","first-page":"763","DOI":"10.3844\/jcssp.2013.763.770","volume":"9","author":"H Abu-Dalbouh","year":"2013","unstructured":"Abu-Dalbouh H. A questionnaire approach based on the technology acceptance model for mobile tracking on patient progress applications. J Comput Sci. 2013;9(6):763\u201370.","journal-title":"J Comput Sci"},{"key":"4056_CR19","doi-asserted-by":"crossref","unstructured":"Baharum A, Amirul S, Yusop N, Halamy S, Fabeil N, Ramli R. Development of questionnaire to measure user acceptance towards user interface design. Advances in visual informatics: 5th international visual informatics conference, IVIC 2017; November 28\u201330, 2017: Springer International Publishing; 2017. p. 531\u2009\u2013\u200943.","DOI":"10.1007\/978-3-319-70010-6_49"},{"issue":"6","key":"4056_CR20","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1007\/s11165-016-9602-2","volume":"48","author":"KS Taber","year":"2018","unstructured":"Taber KS. The use of cronbach\u2019s alpha when developing and reporting research instruments in science education. Res Sci Educ. 2018;48(6):1273\u201396.","journal-title":"Res Sci Educ"},{"issue":"1","key":"4056_CR21","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1136\/eb-2012-100645","volume":"18","author":"M Power","year":"2013","unstructured":"Power M, Fell G, Wright M. Principles for high-quality, high-value testing. Evid Based Med. 2013;18(1):5\u201310.","journal-title":"Evid Based Med"},{"issue":"1","key":"4056_CR22","first-page":"56","volume":"17","author":"H Uma","year":"2018","unstructured":"Uma H, Nausheer A. Identification of dentine sialophosphoprotein in gingival crevicular fluid to assess root resorption using three piece base arch. IOSR J Dent Med Sci (IOSR-JDMS). 2018;17(1):56\u201363.","journal-title":"IOSR J Dent Med Sci (IOSR-JDMS)"},{"key":"4056_CR23","doi-asserted-by":"crossref","unstructured":"Zain MNM, Yusof ZM, Yazid F, Ashari A, Wong KSH, Lee WJ et al. Absorption spectrum analysis of dentine sialophosphoprotein (DSPP) in orthodontic patient. AIP Conference Proceedings; 2020:020007.","DOI":"10.1063\/1.5142099"},{"issue":"2","key":"4056_CR24","first-page":"175","volume":"72","author":"N Brezniak","year":"2002","unstructured":"Brezniak N, Wasserstein A. Orthodontically induced inflammatory root resorption. Part I: the basic science aspects. Angle Orthod. 2002;72(2):175\u20139.","journal-title":"Angle Orthod"},{"issue":"4","key":"4056_CR25","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1111\/ocr.12329","volume":"22","author":"F Tarallo","year":"2019","unstructured":"Tarallo F, Chimenti C, Paiella G, Cordaro M, Tepedino M. Biomarkers in the gingival crevicular fluid used to detect root resorption in patients undergoing orthodontic treatment: a systematic review. Orthod Craniofac Res. 2019;22(4):236\u201347.","journal-title":"Orthod Craniofac Res"},{"issue":"4","key":"4056_CR26","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1016\/j.ajodo.2009.06.021","volume":"137","author":"B Weltman","year":"2010","unstructured":"Weltman B, Vig KW, Fields HW, Shanker S, Kaizar EE. Root resorption associated with orthodontic tooth movement: a systematic review. Am J Orthod Dentofac Orthop. 2010;137(4):462\u201376.","journal-title":"Am J Orthod Dentofac Orthop"},{"issue":"3","key":"4056_CR27","doi-asserted-by":"publisher","first-page":"147","DOI":"10.5624\/isd.2012.42.3.147","volume":"42","author":"K Nanekrungsan","year":"2012","unstructured":"Nanekrungsan K, Patanaporn V, Janhom A, Korwanich N. External apical root resorption in maxillary incisors in orthodontic patients: associated factors and radiographic evaluation. Imaging Sci Dentistry. 2012;42(3):147\u201354.","journal-title":"Imaging Sci Dentistry"}],"container-title":["BMC Oral Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-024-04056-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12903-024-04056-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-024-04056-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T14:02:37Z","timestamp":1709388157000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcoralhealth.biomedcentral.com\/articles\/10.1186\/s12903-024-04056-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,2]]},"references-count":27,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["4056"],"URL":"https:\/\/doi.org\/10.1186\/s12903-024-04056-5","relation":{},"ISSN":["1472-6831"],"issn-type":[{"value":"1472-6831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,2]]},"assertion":[{"value":"9 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 February 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was approved by the Research Ethical Committee of Universiti Kebangsaan Malaysia (UKM\/PPI\/111\/8\/JEP-2021-423). This study was conducted in accordance with the principles of the Declaration of Helsinki, and all patients provided written informed consent prior to enrolment.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"298"}}