{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T10:13:07Z","timestamp":1767262387822},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Oral Health"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>The aim of this study was to evaluate the effect of the use of different NaOCl concentrations (1%, 2.5%, and 5.25%) during root canal treatment of molar teeth with symptomatic irreversible pulpitis on the change of the IL-8 level in gingival crevicular fluid (GFC).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>GCF sampling was performed on experimental tooth with irreversible pulpitis before and after treatment and also contralateral healthy tooth of 54 patients. The patients were divided into three groups according to concentration of NaOCl solution (<jats:italic>n<\/jats:italic>\u2009=\u200918); 1%, 2.5%, and 5.25% NaOCl solution. GCF sampling from experimental teeth was repeated one week after root canal treatment. Statistical analysis was performed using Mann-Whitney U, Wilcoxon test, one-way ANOVA and Pearson correlation analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>There was a significant correlation between IL-8 levels in GCF samples taken from teeth with pulpitis before treatment and from healthy contralateral teeth (<jats:italic>p<\/jats:italic>\u2009=\u2009.000). Furthermore, the pretreatment IL-8 level was significantly higher than the posttreatment IL-8 level(<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The effect of the NaOCl concentration on the change in IL-8 level betweeen pre and post treatment was not found statistically significant (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The use of NaOCl during root canal treatment can effectively reduce the levels of IL-8 in GCF and improve clinical outcomes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Trial registration<\/jats:title>\n                <jats:p>This study was registred in the Institutional Review Board and the Ethics Committee of the University (No:11) on 15\/12\/2021.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12903-024-04128-6","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T12:02:14Z","timestamp":1711540934000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Interleukin-8 (IL-8) levels in gingival crevicular fluid during root canal treatment of molar teeth with symptomatic irreversible pulpitis: impact of varying sodium hypochlorite concentrations"],"prefix":"10.1186","volume":"24","author":[{"given":"Esin","family":"\u00d6zlek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gizem","family":"Kad\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasser","family":"Shoshaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuf","family":"Saed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130smet","family":"Meydan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,3,27]]},"reference":[{"issue":"2","key":"4128_CR1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/ni1158","volume":"6","author":"J Galli Stephen","year":"2005","unstructured":"Galli Stephen J, Nakae S, Tsai M. Mast cells in the development of adaptive immune responses. Nat Immunol. 2005;6(2):135\u201342.","journal-title":"Nat Immunol"},{"issue":"12","key":"4128_CR2","doi-asserted-by":"publisher","first-page":"6785","DOI":"10.1128\/IAI.68.12.6785-6789.2000","volume":"68","author":"L Hahn Chin","year":"2000","unstructured":"Hahn Chin L, Best Al M, Tew John G. Cytokine induction by Streptococcus mutans and pulpal pathogenesis. Infect Immun. 2000;68(12):6785\u20139.","journal-title":"Infect Immun"},{"issue":"10","key":"4128_CR3","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1016\/j.joen.2020.07.013","volume":"46","author":"G Akbal Dincer","year":"2020","unstructured":"Akbal Dincer G, Erdemir A, Kisa U, Comparison of Neurokinin A, Substance P. Interleukin 8, and Matrix Metalloproteinase-8 changes in pulp tissue and gingival crevicular fluid samples of healthy and symptomatic irreversible pulpitis teeth. J Endod. 2020;46(10):1428\u201337.","journal-title":"J Endod"},{"issue":"5","key":"4128_CR4","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1590\/S1678-77572009000500031","volume":"17","author":"O Silva Alessandra Cec\u00edlia","year":"2009","unstructured":"Silva Alessandra Cec\u00edlia O, Faria Miriam R, Fontes A, Campos Marcia S, Cavalcanti Bruno N. Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps. J Appl Oral Sci. 2009;17(5):527\u201332.","journal-title":"J Appl Oral Sci"},{"issue":"2","key":"4128_CR5","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/S1079-2104(99)70118-6","volume":"88","author":"TJ Huang George","year":"1999","unstructured":"Huang George TJ, Potente Anthony P, Kim Jang W, Chugal N, Zhang X. Increased interleukin-8 expression in inflamed human dental pulps. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999;88(2):214\u201320.","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"issue":"6","key":"4128_CR6","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1111\/iej.12030","volume":"46","author":"M Elsalhy","year":"2013","unstructured":"Elsalhy M, Azizieh F, Raghupathy R. Cytokines as diagnostic markers of pulpal inflammation. Int Endod J. 2013;46(6):573\u201380.","journal-title":"Int Endod J"},{"issue":"3\u20134","key":"4128_CR7","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/S1043-4666(03)00116-9","volume":"22","author":"M Zehnder","year":"2003","unstructured":"Zehnder M, Delaleu N, Du Y, Bickel M. Cytokine gene expression - part of host defence in pulpitis. Cytokine. 2003;22(3\u20134):84\u20138.","journal-title":"Cytokine"},{"issue":"2","key":"4128_CR8","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.joen.2007.10.022","volume":"34","author":"V Karapanou","year":"2008","unstructured":"Karapanou V, Kempuraj D, Theoharides Theoharis C. Interleukin-8 is increased in gingival crevicular fluid from patients with acute pulpitis. J Endod. 2008;34(2):148\u201351.","journal-title":"J Endod"},{"issue":"7","key":"4128_CR9","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1016\/j.joen.2017.02.009","volume":"43","author":"M Zanini","year":"2017","unstructured":"Zanini M, Meyer E, Simon S. Pulp inflammation diagnosis from clinical to Inflammatory mediators: a systematic review. J Endod. 2017;43(7):1033\u201351.","journal-title":"J Endod"},{"key":"4128_CR10","doi-asserted-by":"crossref","unstructured":"Ozlek E, Gunduz H, Kadi G, Ta\u015fan A, Akkol E. The effect of solution and gel forms of sodium hypochlorite on postoperative pain: a randomized clinical trial. J Appl Oral Sci. 2021;29.","DOI":"10.1590\/1678-7757-2020-0998"},{"issue":"6","key":"4128_CR11","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1111\/j.1875-595X.2008.tb00354.x","volume":"58","author":"Z Mohammadi","year":"2008","unstructured":"Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Int Dent J. 2008;58(6):329\u201341.","journal-title":"Int Dent J"},{"issue":"6","key":"4128_CR12","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1111\/iej.13294","volume":"53","author":"V Nagendrababu","year":"2020","unstructured":"Nagendrababu V, Duncan HF, Bj\u00f8rndal L, et al. PRIRATE 2020 guidelines for reporting randomized trials in endodontics: a consensus-based development. Int Endod J. 2020;53(6):764\u201373.","journal-title":"Int Endod J"},{"issue":"12","key":"4128_CR13","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1016\/j.joen.2019.09.011","volume":"45","author":"E Uluk\u00f6yl\u00fc","year":"2019","unstructured":"Uluk\u00f6yl\u00fc E, Karata\u015f E, Albayrak M, Bay\u0131r Y. Effect of calcium hydroxide alone or in combination with ibuprofen and ciprofloxacin on nuclear factor Kappa B Ligand and Osteoprotegerin Level in Periapical lesions: a Randomized Controlled Clinical Study. J Endod. 2019;45(12):1489\u201395.","journal-title":"J Endod"},{"issue":"3","key":"4128_CR14","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1111\/j.0143-2885.2004.00782.x","volume":"37","author":"SH Park","year":"2004","unstructured":"Park SH, Hsiao GYW, Huang GTJ. Role of substance P and calcitonin gene-related peptide in the regulation of interleukin-8 and monocyte chemotactic protein-1 expression in human dental pulp. Int Endod J. 2004;37(3):185\u201392.","journal-title":"Int Endod J"},{"issue":"11","key":"4128_CR15","doi-asserted-by":"publisher","first-page":"1738","DOI":"10.1016\/j.joen.2020.07.020","volume":"46","author":"B Orlowski Nayra","year":"2020","unstructured":"Orlowski Nayra B, Schimdt Tamer F, Teixeira Cleonice da S, et al. Smear layer removal using passive ultrasonic irrigation and different concentrations of sodium hypochlorite. J Endod. 2020;46(11):1738\u201344.","journal-title":"J Endod"},{"issue":"6","key":"4128_CR16","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1097\/00004770-200006000-00006","volume":"26","author":"F Siqueira Jos\u00e9","year":"2000","unstructured":"Siqueira Jos\u00e9 F, R\u00f4\u00e7as Isabela N, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 2000;26(6):331\u20134.","journal-title":"J Endod"},{"issue":"2","key":"4128_CR17","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1046\/j.1365-2591.1999.00196.x","volume":"32","author":"H Ayhan","year":"1999","unstructured":"Ayhan H, Sultan N, \u00c7irak M, Ruhi MZ, Bodur H. Antimicrobial effects of various endodontic irrigants on selected microorganisms. Int Endod J. 1999;32(2):99\u2013102.","journal-title":"Int Endod J"},{"issue":"12","key":"4128_CR18","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1016\/S0099-2399(06)81331-2","volume":"18","author":"JC Baumgartner","year":"1992","unstructured":"Baumgartner JC, Cuenin Paul R. Efficacy of several concentrations of sodium hypochlorite for root canal irrigation. J Endod. 1992;18(12):605\u201312.","journal-title":"J Endod"},{"issue":"8","key":"4128_CR19","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1097\/00004770-200208000-00003","volume":"28","author":"M Goldsmith","year":"2002","unstructured":"Goldsmith M, Gulabivala K, Knowles JC. The effect of sodium hypochlorite irrigant concentration on tooth surface strain. J Endod. 2002;28(8):575\u20139.","journal-title":"J Endod"},{"key":"4128_CR20","first-page":"106","volume":"11","author":"I Laxmi","year":"2021","unstructured":"Laxmi I, Kumar S, Sciences SR. Comparison of inflammatory markers in saliva before and after endodontic treatment. Ann Med Health Sci Res. 2021;11:106\u201312.","journal-title":"Ann Med Health Sci Res"},{"issue":"1","key":"4128_CR21","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1111\/aej.12340","volume":"46","author":"J Evangelin","year":"2020","unstructured":"Evangelin J, Sherwood IA, Abbott PV, Uthandakalaipandian R, Velu V. Influence of different irrigants on substance P and IL-8 expression for single visit root canal treatment in acute irreversible pulpitis. Aust Endod J. 2020;46(1):17\u201325.","journal-title":"Aust Endod J"}],"container-title":["BMC Oral Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-024-04128-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12903-024-04128-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-024-04128-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T12:03:20Z","timestamp":1711541000000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcoralhealth.biomedcentral.com\/articles\/10.1186\/s12903-024-04128-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,27]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["4128"],"URL":"https:\/\/doi.org\/10.1186\/s12903-024-04128-6","relation":{},"ISSN":["1472-6831"],"issn-type":[{"value":"1472-6831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,27]]},"assertion":[{"value":"9 October 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 March 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The use of extracted teeth was approved by the Institutional Review Board and Ethics committee of The University of Yuzuncu Yil (11\u201315\/12\/2021) and informed consent was obtained from all subjects and\/or their legal guardian(s).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"392"}}