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The pathogenesis of pain is still unclear. Our objective was to assess Whether Voltage-gated calcium channel \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    \u03b4-1(Cav\u03b12\u03b4-1) is related to myofascial orofacial pain. Rats were divided into the masseter tendon ligation group and the sham group. Compared with the sham group, the mechanical pain threshold of the masseter tendon ligation group was reduced on the 4th, 7th, 10th and 14th day after operation(\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05). On the 14th day after operation, Cav\u03b12\u03b4-1 mRNA expression levels in trigeminal ganglion (TG) and the trigeminal spinal subnucleus caudalis and C1-C2 spinal cervical dorsal horn (Vc\/C\n                    <jats:sub>2<\/jats:sub>\n                    ) of the masseter tendon ligation group were increased (\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>TG<\/jats:italic>\n                    <\/jats:sub>\n                    =0.021,\n                    <jats:italic>P<\/jats:italic>\n                    <jats:sub>Vc\/C2<\/jats:sub>\n                    =0.012). Rats were divided into three groups. On the 4th day after ligating the superficial tendon of the left masseter muscle of the rats, 10 ul Cav\u03b12\u03b4-1 antisense oligonucleotide, 10 ul Cav\u03b12\u03b4-1 mismatched oligonucleotides and 10 ul normal saline was separately injected into the left masseter muscle of rats in Cav\u03b12\u03b4-1 antisense oligonucleotide group, Cav\u03b12\u03b4-1 mismatched oligonucleotides group and normal saline control group twice a day for 4 days. The mechanical pain threshold of the Cav\u03b12\u03b4-1 antisense oligonucleotides group was higher than Cav\u03b12\u03b4-1 mismatched oligonucleotides group on the 7th and 10th day after operation (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01). After PC12 cells were treated with lipopolysaccharide, Cav\u03b12\u03b4-1 mRNA expression level increased (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001). Cav\u03b12\u03b4-1 may be involved in the occurrence and development in myofascial orofacial pain.\n                  <\/jats:p>","DOI":"10.1186\/s12903-024-04338-y","type":"journal-article","created":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T12:01:29Z","timestamp":1715515289000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The role of voltage-gated calcium channel \u03b12\u03b4-1 in the occurrence and development in myofascial orofacial pain"],"prefix":"10.1186","volume":"24","author":[{"given":"Yang","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,12]]},"reference":[{"key":"4338_CR1","doi-asserted-by":"crossref","unstructured":"International Classification of Orofacial Pain, 1st edition (ICOP). 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All the rats were male. Our experiments are reported in accordance with ARRIVE guidelines and approved by School and Hospital of Stomatology, China Medical University, Ethics No. 2018067. The experimental operation and animal feeding complianced the basic regulations for relevant guidelines (e.g. the revised Animals (Scientific Procedures) Act 1986 in the UK and Directive 2010\/63\/EU in Europe).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"552"}}