{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T14:14:29Z","timestamp":1759760069524,"version":"build-2065373602"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T00:00:00Z","timestamp":1759708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T00:00:00Z","timestamp":1759708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Oral Health"],"DOI":"10.1186\/s12903-025-06932-0","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T13:34:29Z","timestamp":1759757669000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Salivary mitofusin levels in periodontal disease: a cross-sectional case-control study"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9425-5615","authenticated-orcid":false,"given":"\u00d6mer F.","family":"Okumu\u015f","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1685-5332","authenticated-orcid":false,"given":"Nezahat","family":"Kurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2679-9100","authenticated-orcid":false,"given":"Alpdo\u011fan","family":"Kantarc\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,6]]},"reference":[{"key":"6932_CR1","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1042\/EBC20170104","volume":"62","author":"L Tilokani","year":"2018","unstructured":"Tilokani L, Nagashima S, Paupe V, Prudent J. Mitochondrial dynamics: overview of molecular mechanisms. Essays Biochem. 2018;62:341\u201360. https:\/\/doi.org\/10.1042\/EBC20170104.","journal-title":"Essays Biochem"},{"key":"6932_CR2","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1002\/JPER.23-0072","volume":"95","author":"\u00d6A K\u0131rm\u0131z\u0131g\u00fcl","year":"2024","unstructured":"K\u0131rm\u0131z\u0131g\u00fcl \u00d6A, Sabanci A, Di\u015fli F, Y\u0131ld\u0131z S, Milward MR, Aral K. Evaluation of the role of mitofusin-1 and mitofusin-2 in periodontal disease. J Periodontol. 2024;95:64\u201373. https:\/\/doi.org\/10.1002\/JPER.23-0072.","journal-title":"J Periodontol"},{"key":"6932_CR3","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1038\/nature07534","volume":"456","author":"OM de Brito","year":"2008","unstructured":"de Brito OM, Scorrano L. Mitofusin 2 tethers Endoplasmic reticulum to mitochondria. Nature. 2008;456:605\u201310. https:\/\/doi.org\/10.1038\/nature07534.","journal-title":"Nature"},{"key":"6932_CR4","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.bbrc.2018.12.143","volume":"509","author":"Y Chen","year":"2019","unstructured":"Chen Y, Ji Y, Jin X, Sun X, Zhang X, Chen Y, et al. Mitochondrial abnormalities are involved in periodontal ligament fibroblast apoptosis induced by oxidative stress. Biochem Biophys Res Commun. 2019;509:483\u201390. https:\/\/doi.org\/10.1016\/j.bbrc.2018.12.143.","journal-title":"Biochem Biophys Res Commun"},{"key":"6932_CR5","doi-asserted-by":"publisher","first-page":"105173","DOI":"10.1016\/j.archoralbio.2021.105173","volume":"128","author":"K Aral","year":"2021","unstructured":"Aral K, Milward MR, Cooper PR. Gene expression profiles of mitochondria-endoplasmic reticulum tethering in human gingival fibroblasts in response to periodontal pathogens. Arch Oral Biol. 2021;128:105173. https:\/\/doi.org\/10.1016\/j.archoralbio.2021.105173.","journal-title":"Arch Oral Biol"},{"key":"6932_CR6","doi-asserted-by":"publisher","first-page":"17963","DOI":"10.1073\/pnas.1312571110","volume":"110","author":"T Ichinohe","year":"2013","unstructured":"Ichinohe T, Yamazaki T, Koshiba T, Yanagi Y. Mitochondrial protein Mitofusin 2 is required for NLRP3 inflammasome activation after RNA virus infection. Proc Natl Acad Sci. 2013;110:17963\u20138. https:\/\/doi.org\/10.1073\/pnas.1312571110.","journal-title":"Proc Natl Acad Sci"},{"key":"6932_CR7","doi-asserted-by":"publisher","unstructured":"Khodzhaeva V, Schreiber Y, Geisslinger G, Brandes RP, Br\u00fcne B, Namgaladze D. Mitofusin 2 deficiency causes Pro-Inflammatory effects in human primary macrophages. Front Immunol. 2021;12:723683. https:\/\/doi.org\/10.3389\/fimmu.2021.723683.","DOI":"10.3389\/fimmu.2021.723683"},{"key":"6932_CR8","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1902\/jop.2014.130654","volume":"85","author":"E Baltac\u0131o\u011flu","year":"2014","unstructured":"Baltac\u0131o\u011flu E, Yuva P, Ayd\u0131n G, Alver A, Kahraman C, Karabulut E, et al. Lipid peroxidation levels and total oxidant\/antioxidant status in serum and saliva from patients with chronic and aggressive periodontitis. Oxidative stress index: A new biomarker for periodontal disease?? J Periodontol. 2014;85:1432\u201341. https:\/\/doi.org\/10.1902\/jop.2014.130654.","journal-title":"J Periodontol"},{"key":"6932_CR9","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1139\/cjpp-2014-0361","volume":"93","author":"P Delmotte","year":"2015","unstructured":"Delmotte P, Sieck GC. Interaction between endoplasmic\/sarcoplasmic reticulum stress (ER\/SR stress), mitochondrial signaling and Ca2\u2009+\u2009regulation in airway smooth muscle (ASM). Can J Physiol Pharmacol. 2015;93:97\u2013110. https:\/\/doi.org\/10.1139\/cjpp-2014-0361.","journal-title":"Can J Physiol Pharmacol"},{"key":"6932_CR10","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1111\/jre.13132","volume":"58","author":"OK \u015eim\u015fek","year":"2023","unstructured":"\u015eim\u015fek OK, Baser U, \u00d6zg\u00fcnler \u00d6, Demirci O, Aydin AF, Kucukgergin C, et al. Comparison of oxidative stress markers in the saliva, gingival crevicular fluid, and serum samples of pregnant women with gestational diabetes and healthy pregnant women. J Periodontal Res. 2023;58:745\u201354. https:\/\/doi.org\/10.1111\/jre.13132.","journal-title":"J Periodontal Res"},{"key":"6932_CR11","doi-asserted-by":"publisher","first-page":"E2174","DOI":"10.1073\/pnas.1504880112","volume":"112","author":"R Filadi","year":"2015","unstructured":"Filadi R, Greotti E, Turacchio G, Luini A, Pozzan T, Pizzo P. Mitofusin 2 ablation increases Endoplasmic reticulum-mitochondria coupling. Proc Natl Acad Sci U S A. 2015;112:E2174\u20132181. https:\/\/doi.org\/10.1073\/pnas.1504880112.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"6932_CR12","doi-asserted-by":"publisher","unstructured":"Tur J, Pereira-Lopes S, Vico T, Mar\u00edn EA, Mu\u00f1oz JP, Hern\u00e1ndez-Alvarez M, et al. Mitofusin 2 in macrophages links mitochondrial ROS production, cytokine release, phagocytosis, autophagy, and bactericidal activity. Cell Rep. 2020;32:108079. https:\/\/doi.org\/10.1016\/j.celrep.2020.108079.","DOI":"10.1016\/j.celrep.2020.108079"},{"key":"6932_CR13","doi-asserted-by":"publisher","first-page":"jcs253443","DOI":"10.1242\/jcs.253443","volume":"134","author":"S Han","year":"2021","unstructured":"Han S, Zhao F, Hsia J, Ma X, Liu Y, Torres S, et al. The role of Mfn2 in the structure and function of Endoplasmic reticulum-mitochondrial tethering in vivo. J Cell Sci. 2021;134:jcs253443. https:\/\/doi.org\/10.1242\/jcs.253443.","journal-title":"J Cell Sci"},{"key":"6932_CR14","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.1111\/jcmm.12863","volume":"20","author":"NS Leal","year":"2016","unstructured":"Leal NS, Schreiner B, Pinho CM, Filadi R, Wiehager B, Karlstr\u00f6m H, et al. Mitofusin-2 knockdown increases ER-mitochondria contact and decreases amyloid \u03b2-peptide production. J Cell Mol Med. 2016;20:1686\u201395. https:\/\/doi.org\/10.1111\/jcmm.12863.","journal-title":"J Cell Mol Med"},{"key":"6932_CR15","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3109\/00016356408993968","volume":"22","author":"J Silness","year":"1964","unstructured":"Silness J, Loe H, PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. Acta Odontol Scand. 1964;22:121\u201335. https:\/\/doi.org\/10.3109\/00016356408993968.","journal-title":"Acta Odontol Scand"},{"key":"6932_CR16","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1902\/jop.1967.38.6.610","volume":"38","author":"H L\u00f6e","year":"1967","unstructured":"L\u00f6e H. The gingival index, the plaque index and the retention index systems. J Periodontol. 1967;38:610\u20136. https:\/\/doi.org\/10.1902\/jop.1967.38.6.610.","journal-title":"J Periodontol"},{"key":"6932_CR17","first-page":"229","volume":"25","author":"J Ainamo","year":"1975","unstructured":"Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975;25:229\u201335.","journal-title":"Int Dent J"},{"issue":"Suppl 1","key":"6932_CR18","doi-asserted-by":"publisher","first-page":"S74","DOI":"10.1002\/JPER.17-0719","volume":"89","author":"ILC Chapple","year":"2018","unstructured":"Chapple ILC, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: consensus report of workgroup 1 of the 2017 world workshop on the classification of periodontal and Peri-Implant diseases and conditions. J Periodontol. 2018;89(Suppl 1):S74\u201384. https:\/\/doi.org\/10.1002\/JPER.17-0719.","journal-title":"J Periodontol"},{"issue":"Suppl 1","key":"6932_CR19","doi-asserted-by":"publisher","first-page":"S173","DOI":"10.1002\/JPER.17-0721","volume":"89","author":"PN Papapanou","year":"2018","unstructured":"Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: consensus report of workgroup 2 of the 2017 world workshop on the classification of periodontal and Peri-Implant diseases and conditions. J Periodontol. 2018;89(Suppl 1):S173\u201382. https:\/\/doi.org\/10.1002\/JPER.17-0721.","journal-title":"J Periodontol"},{"key":"6932_CR20","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1111\/j.1749-6632.1993.tb18343.x","volume":"694","author":"M Navazesh","year":"1993","unstructured":"Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;694:72\u20137. https:\/\/doi.org\/10.1111\/j.1749-6632.1993.tb18343.x.","journal-title":"Ann N Y Acad Sci"},{"key":"6932_CR21","doi-asserted-by":"publisher","unstructured":"Sanz M, Herrera D, Kebschull M, Chapple I, Jepsen S, Berglundh T, et al. Treatment of stage I\u2013III periodontitis\u2014The EFP S3 level clinical practice guideline. J Clin Periodontol. 2020;47(Suppl 22):4\u201360. https:\/\/doi.org\/10.1111\/jcpe.13290.","DOI":"10.1111\/jcpe.13290"},{"key":"6932_CR22","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1186\/s12903-021-01600-5","volume":"21","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Kang N, Xue F, Qiao J, Duan J, Chen F, et al. Evaluation of salivary biomarkers for the diagnosis of periodontitis. BMC Oral Health. 2021;21:266. https:\/\/doi.org\/10.1186\/s12903-021-01600-5.","journal-title":"BMC Oral Health"},{"key":"6932_CR23","doi-asserted-by":"publisher","first-page":"71","DOI":"10.4196\/kjpp.2012.16.1.71","volume":"16","author":"K-S Park","year":"2012","unstructured":"Park K-S, Wiederkehr A, Wollheim CB. Defective mitochondrial function and motility due to Mitofusin 1 overexpression in insulin secreting cells. Korean J Physiol Pharmacol Off J Korean Physiol Soc Korean Soc Pharmacol. 2012;16:71\u20137. https:\/\/doi.org\/10.4196\/kjpp.2012.16.1.71.","journal-title":"Korean J Physiol Pharmacol Off J Korean Physiol Soc Korean Soc Pharmacol"},{"key":"6932_CR24","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.phrs.2018.01.003","volume":"128","author":"R Filadi","year":"2018","unstructured":"Filadi R, Greotti E, Pizzo P. Highlighting the Endoplasmic reticulum-mitochondria connection: focus on Mitofusin 2. Pharmacol Res. 2018;128:42\u201351. https:\/\/doi.org\/10.1016\/j.phrs.2018.01.003.","journal-title":"Pharmacol Res"},{"key":"6932_CR25","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.15252\/embj.201593084","volume":"35","author":"D Sebasti\u00e1n","year":"2016","unstructured":"Sebasti\u00e1n D, Sorianello E, Segal\u00e9s J, Irazoki A, Ruiz-Bonilla V, Sala D, et al. Mfn2 deficiency links age\u2010related sarcopenia and impaired autophagy to activation of an adaptive mitophagy pathway. EMBO J. 2016;35:1677\u201393. https:\/\/doi.org\/10.15252\/embj.201593084.","journal-title":"EMBO J"},{"key":"6932_CR26","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/j.jobcr.2023.05.003","volume":"13","author":"S Nisha","year":"2023","unstructured":"Nisha S, Bettahalli Shivamallu A, Prashant A, Shashikumar P, Ganganna A, Das D. Effect of Non surgical periodontal therapy and vitamin C supplementation on total antioxidant capacity in patients with chronic generalised periodontitis - A randomised controlled trial. J Oral Biol Craniofac Res. 2023;13:511\u20136. https:\/\/doi.org\/10.1016\/j.jobcr.2023.05.003.","journal-title":"J Oral Biol Craniofac Res"},{"key":"6932_CR27","doi-asserted-by":"publisher","unstructured":"Zhang T, Andrukhov O, Haririan H, M\u00fcller-Kern M, Liu S, Liu Z, et al. Total antioxidant capacity and total oxidant status in saliva of periodontitis patients in relation to bacterial load. Front Cell Infect Microbiol. 2016;5:97. https:\/\/doi.org\/10.3389\/fcimb.2015.00097.","DOI":"10.3389\/fcimb.2015.00097"},{"key":"6932_CR28","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.archoralbio.2017.09.006","volume":"84","author":"U Ko\u0142odziej","year":"2017","unstructured":"Ko\u0142odziej U, Maciejczyk M, Nikli\u0144ska W, Waszkiel D, \u017bendzian-Piotrowska M, \u017bukowski P, et al. Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats. Arch Oral Biol. 2017;84:6\u201312. https:\/\/doi.org\/10.1016\/j.archoralbio.2017.09.006.","journal-title":"Arch Oral Biol"},{"key":"6932_CR29","doi-asserted-by":"publisher","first-page":"548","DOI":"10.3390\/jcm9020548","volume":"9","author":"A Zalewska","year":"2020","unstructured":"Zalewska A, Kossakowska A, Taranta-Janusz K, Zi\u0119ba S, Fejfer K, Salamonowicz M, et al. Dysfunction of salivary glands, disturbances in salivary antioxidants and increased oxidative damage in saliva of overweight and obese adolescents. J Clin Med. 2020;9:548. https:\/\/doi.org\/10.3390\/jcm9020548.","journal-title":"J Clin Med"},{"key":"6932_CR30","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1111\/j.1600-0757.2006.00178.x","volume":"43","author":"ILC Chapple","year":"2007","unstructured":"Chapple ILC, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 2007;43:160\u2013232. https:\/\/doi.org\/10.1111\/j.1600-0757.2006.00178.x.","journal-title":"Periodontol 2000"},{"key":"6932_CR31","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.freeradbiomed.2015.04.005","volume":"85","author":"J Wang","year":"2015","unstructured":"Wang J, Schipper HM, Velly AM, Mohit S, Gornitsky M. Salivary biomarkers of oxidative stress: A critical review. Free Radic Biol Med. 2015;85:95\u2013104. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2015.04.005.","journal-title":"Free Radic Biol Med"},{"key":"6932_CR32","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1111\/jre.12850","volume":"56","author":"P Pani","year":"2021","unstructured":"Pani P, Tsilioni I, McGlennen R, Brown CA, Hawley CE, Theoharides TC, et al. IL-1B(3954) polymorphism and red complex bacteria increase IL-1\u03b2 (GCF) levels in periodontitis. J Periodontal Res. 2021;56:501\u201311. https:\/\/doi.org\/10.1111\/jre.12850.","journal-title":"J Periodontal Res"},{"key":"6932_CR33","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1111\/j.1600-0765.2004.00744.x","volume":"39","author":"P-F Wei","year":"2004","unstructured":"Wei P-F, Ho K-Y, Ho Y-P, Wu Y-M, Yang Y-H, Tsai C-C. The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1\u03b2 in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases. J Periodontal Res. 2004;39:287\u201393. https:\/\/doi.org\/10.1111\/j.1600-0765.2004.00744.x.","journal-title":"J Periodontal Res"},{"key":"6932_CR34","doi-asserted-by":"publisher","first-page":"1851420","DOI":"10.1155\/2016\/1851420","volume":"2016","author":"T Thoudam","year":"2016","unstructured":"Thoudam T, Jeon J-H, Ha C-M, Lee I-K. Role of Mitochondria-Associated Endoplasmic reticulum membrane in Inflammation-Mediated metabolic diseases. Mediators Inflamm. 2016;2016:1851420. https:\/\/doi.org\/10.1155\/2016\/1851420.","journal-title":"Mediators Inflamm"}],"container-title":["BMC Oral Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-025-06932-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12903-025-06932-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12903-025-06932-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T13:34:29Z","timestamp":1759757669000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcoralhealth.biomedcentral.com\/articles\/10.1186\/s12903-025-06932-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,6]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6932"],"URL":"https:\/\/doi.org\/10.1186\/s12903-025-06932-0","relation":{},"ISSN":["1472-6831"],"issn-type":[{"value":"1472-6831","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,6]]},"assertion":[{"value":"4 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This research has been conducted in full accordance with the ethical principles outlined in the World Medical Association Declaration of Helsinki (version 2008). All interventions were conducted with the understanding and written consent of each participant in accordance with the aforementioned principles. Ethical approval was obtained from the Clinical Research Ethics Committee of Erzincan University (decision dated March 21, 2024, numbered 2024-04\/04, Erzincan, Turkey).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1542"}}