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This study addresses the challenges by exploring antimicrobial peptides (AMPs) derived from ameloblastin (AMBN), a protein integral to dental biomineralization and categorized as an intrinsically disordered protein. In humans, the AMBN gene encodes two isoforms, ISO I and ISO II, with distinct but not fully understood functions. Four AMBN ISO I\u2013derived peptides (A, Am, B, Bm) were designed, synthesized, and tested for antimicrobial and antibiofilm activity. Peptides A and Am moderately inhibited biofilms of\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    ,\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , and\n                    <jats:italic>E. coli<\/jats:italic>\n                    (MBIC\u2085\u2080 within 50\u2013300 \u00b5M), including resistant isolates, while B and Bm were more effective against Gram-positive strains, showing the strongest effect against methicillin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    CNCTC 6271. Cytotoxicity assays showed\u2009&gt;\u200990% cell viability at 50 \u00b5M and IC\u2085\u2080 &gt;100 \u00b5M for HCT116 and &gt;\u2009300 \u00b5M for HUVEC cells, with haemolytic activity\u2009&gt;\u2009300 \u00b5M. Stable immobilization of peptides on titanium surfaces was confirmed by XPS and QCM-D techniques, supporting their potential as low-toxicity antimicrobial coatings for medical implants.\n                  <\/jats:p>","DOI":"10.1186\/s12903-025-07433-w","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T05:37:50Z","timestamp":1764653870000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial peptides derived from human ameloblastin targeting biofilms"],"prefix":"10.1186","volume":"26","author":[{"given":"Veronika","family":"Vetyskova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Kasparova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Bednarova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"Hajek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Luxa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro Barrantes","family":"Bautista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane Elin","family":"Reseland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Matatkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Masak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Vondrasek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristyna","family":"Vydra Bousova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"issue":"12","key":"7433_CR1","doi-asserted-by":"publisher","first-page":"4458","DOI":"10.3390\/ijms21124458","volume":"21","author":"A Gil-Bona","year":"2020","unstructured":"Gil-Bona A, Bidlack FB. 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