{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:45:38Z","timestamp":1792259138296,"version":"4.4.0"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Women's Health"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Posterior compartment prolapse is a frequent pelvic floor disorder. While conventional posterior colporrhaphy is widely used, the recurrence rates and limitations in terms of tissue support remain concerns. Human amniotic membrane represents a promising biological scaffold with potent regenerative properties. This study aimed to evaluate the feasibility, safety and effectiveness of using amniotic membrane as a graft in posterior colporrhaphy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This pilot one-arm clinical trial was conducted on women with posterior compartment prolapse at Ain Shams University Hospital during the period (November 2023- August 2025). Twenty-five patients underwent posterior colporrhaphy using gamma-irradiated amniotic membrane as a graft on the rectovaginal septum. We assessed symptoms score via the Pelvic Organ Prolapse Distress Inventory 6 (POPDI-6), sexual function via the Female Sexual Function Index Questionnaire, and pelvic organ prolapse quantification (POP-Q) preoperatively, and at 3 and 6 months post-operatively, and assessed complication rates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The AM graft was successfully implanted in all patients. The overall procedure was associated with reduction in POPDI-6 scores from 68.00\u2009\u00b1\u200914.52 at baseline to 20.67\u2009\u00b1\u20097.93 at 6 months (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), with a mean percentage reduction of 68.22% \u00b1 14.54%. The sexual function score improved from 11.75\u2009\u00b1\u20093.55 to 30.80\u2009\u00b1\u20092.75. Anatomical assessment revealed that 80% of patients achieved POP-Q stage 0, whereas 20% reached stage I by 6 months. No serious complications (wound dehiscence, visceral injury) were reported; however, vaginitis was common, affecting 76% of patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Posterior colporrhaphy with adjuvant gamma-irradiated amniotic membrane graft was associated with some improvement in symptoms, clinical examination, as well as sexual function of the patients. However, this is a one-arm pilot study; further studies are needed to compare this technique with conventional colporrhaphy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial registration<\/jats:title>\n                    <jats:p>Clinicaltrials.gov NCT06117670, on 31\/10\/2023.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12905-026-04919-9","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:04:43Z","timestamp":1792224283000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gamma-irradiated amniotic membrane graft as an adjuvant to posterior colporrhaphy: an exploratory prospective single-arm clinical trial"],"prefix":"10.1186","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-2363","authenticated-orcid":false,"given":"Rania Hassan Mostafa","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4618-3434","authenticated-orcid":false,"given":"Mohab Ahmed Fouad","family":"ElRabat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8807-351X","authenticated-orcid":false,"given":"Nourhan Ashraf","family":"ElSherbiny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5708-977X","authenticated-orcid":false,"given":"Ihab Fouad Serag Eldin","family":"Allam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0955-0696","authenticated-orcid":false,"given":"Ahmed Hesham Abdel","family":"Hakim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1455-523X","authenticated-orcid":false,"given":"Ahmed Mohamed Abbas","family":"Sadek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,17]]},"reference":[{"key":"4919_CR1","doi-asserted-by":"publisher","unstructured":"Mowat A, Maher D, Baessler K, Christmann-Schmid C, Haya N, Maher C. Surgery for women with posterior compartment prolapse. Cochrane Database Syst Rev. 2018;3(3):CD012975. https:\/\/doi.org\/10.1002\/14651858.CD012975.\u00a0Published 2018 Mar 5.","DOI":"10.1002\/14651858.CD012975"},{"issue":"2","key":"4919_CR2","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.ajog.2022.04.046","volume":"227","author":"SFM Schulten","year":"2022","unstructured":"Schulten SFM, Claas-Quax MJ, Weemhoff M, et al. Risk factors for primary pelvic organ prolapse and prolapse recurrence: an updated systematic review and meta-analysis. Am J Obstet Gynecol. 2022;227(2):192\u2013208. https:\/\/doi.org\/10.1016\/j.ajog.2022.04.046.","journal-title":"Am J Obstet Gynecol"},{"issue":"3","key":"4919_CR3","doi-asserted-by":"publisher","first-page":"CD012079","DOI":"10.1002\/14651858.CD012079.pub2","volume":"3","author":"E Yeung","year":"2024","unstructured":"Yeung E, Baessler K, Christmann-Schmid C, et al. Transvaginal mesh or grafts or native tissue repair for vaginal prolapse. Cochrane Database Syst Rev. 2024;3(3):CD012079. https:\/\/doi.org\/10.1002\/14651858.CD012079.pub2. Published 2024 Mar 13.","journal-title":"Cochrane Database Syst Rev"},{"key":"4919_CR4","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1007\/s11934-020-01013-x","volume":"21","author":"AL Merriman","year":"2020","unstructured":"Merriman AL, Kennelly MJ. Biologic Grafts for Use in Pelvic Organ Prolapse Surgery: a Contemporary Review. Curr Urol Rep. 2020;21:52. https:\/\/doi.org\/10.1007\/s11934-020-01013-x.","journal-title":"Curr Urol Rep"},{"issue":"1","key":"4919_CR5","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s00404-026-08315-4","volume":"313","author":"F Beer","year":"2026","unstructured":"Beer F, Schaufelberger P, Friedl TW, Lindner A, Sch\u00fctze S, Janni W, Deniz M. Outcomes and patient satisfaction after pelvic organ prolapse surgery with and without mesh: a retrospective cohort study with prospective follow-up. Arch Gynecol Obstet. 2026;313(1):121. https:\/\/doi.org\/10.1007\/s00404-026-08315-4.","journal-title":"Arch Gynecol Obstet"},{"key":"4919_CR6","doi-asserted-by":"publisher","first-page":"1152068","DOI":"10.3389\/frtra.2023.1152068","volume":"2","author":"N Hofmann","year":"2023","unstructured":"Hofmann N, Rennekampff HO, Salz AK, B\u00f6rgel M. Preparation of human amniotic membrane for transplantation in different application areas. Front Transpl. 2023;2:1152068. https:\/\/doi.org\/10.3389\/frtra.2023.1152068. Published 2023 May 5.","journal-title":"Front Transpl"},{"key":"4919_CR7","doi-asserted-by":"publisher","first-page":"1067480","DOI":"10.3389\/fbioe.2022.1067480","volume":"10","author":"JR Munoz-Torres","year":"2023","unstructured":"Munoz-Torres JR, Mart\u00ednez-Gonz\u00e1lez SB, Lozano-Luj\u00e1n AD, et al. Biological properties and surgical applications of the human amniotic membrane. Front Bioeng Biotechnol. 2023;10:1067480. https:\/\/doi.org\/10.3389\/fbioe.2022.1067480. Published 2023 Jan 9.","journal-title":"Front Bioeng Biotechnol"},{"issue":"1","key":"4919_CR8","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.ajog.2004.12.025","volume":"193","author":"MD Barber","year":"2005","unstructured":"Barber MD, Walters MD, Bump RC. Short forms of two condition-specific quality-of-life questionnaires for women with pelvic floor disorders (PFDI-20 and PFIQ-7). Am J Obstet Gynecol. 2005;193(1):103\u201313. https:\/\/doi.org\/10.1016\/j.ajog.2004.12.025.","journal-title":"Am J Obstet Gynecol"},{"issue":"2","key":"4919_CR9","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1080\/009262300278597","volume":"26","author":"R Rosen","year":"2000","unstructured":"Rosen R, Brown C, Heiman J, et al. The Female Sexual Function Index (FSFI): a multidimensional self-report instrument for the assessment of female sexual function. J Sex Marital Ther. 2000;26(2):191\u2013208. https:\/\/doi.org\/10.1080\/009262300278597.","journal-title":"J Sex Marital Ther"},{"key":"4919_CR10","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1097\/AOG.0000000000000386","volume":"124","author":"SD Reed","year":"2014","unstructured":"Reed SD, Mitchell CM, Joffe H, et al. Sexual function in women on estradiol or venlafaxine for hot flushes: a randomized controlled trial. Obstet Gynecol. 2014;124:233\u201341. https:\/\/doi.org\/10.1097\/AOG.0000000000000386.","journal-title":"Obstet Gynecol"},{"issue":"4","key":"4919_CR11","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1007\/s10561-014-9433-1","volume":"15","author":"N Marsit","year":"2014","unstructured":"Marsit N, Dwejen S, Saad I, et al. 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Published 2019 Mar 30.","journal-title":"Genes Dis"}],"container-title":["BMC Women's Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12905-026-04919-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12905-026-04919-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12905-026-04919-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:02:41Z","timestamp":1792256561000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12905-026-04919-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,17]]},"references-count":16,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4919"],"URL":"https:\/\/doi.org\/10.1186\/s12905-026-04919-9","relation":{},"ISSN":["1472-6874"],"issn-type":[{"value":"1472-6874","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,17]]},"assertion":[{"value":"3 July 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study was performed in accordance with the principles of the Declaration of Helsinki. Approval was granted by the Research Ethical Committee, Faculty of Medicine, Ain Shams University, on 26-03-2023 (FMASU MD 81\/2023).Informed consent was obtained from all participants before recruitment in the study, and after the purpose and procedures of the study were explained.","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"532"}}