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On the basis of literature search, there is less conclusive evidence of the involvement of phytochemical constituents in breast cancer, in particular, animal tumor models. The current study aimed to determine the antitumor and antioxidant activities of <jats:italic>C. nutans<\/jats:italic> extract in 4\u2009T1 tumor-bearing mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p><jats:italic>C. nutans<\/jats:italic> leaves were subjected to methanol extraction and divided into two different concentrations, 200\u2009mg\/kg (low-dose) and 1000\u2009mg\/kg (high-dose). The antitumor effects of <jats:italic>C. nutans<\/jats:italic> extracts were assessed using bone marrow smearing, clonogenic, and splenocyte immunotype analyses. In addition, hematoxylin and eosin, tumor weight and tumor volume profiles also used to indicate apoptosis appearance. Serum cytokine levels were examined using ELISA assay. In addition, nitric oxide assay reflecting antioxidant activity was performed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>From the results obtained, the methanol extract of <jats:italic>C. nutans<\/jats:italic> leaves at 200\u2009mg\/kg (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and 1000\u2009mg\/kg (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) showed a significant decrease in nitric oxide (NO) and malondialdehyde (MDA) levels in the blood. On the other hand, <jats:italic>C. nutans<\/jats:italic> extract (1000\u2009mg\/kg) also showed a significant decrease in the number of mitotic cells, tumor weight, and tumor volume. No inflammatory and adverse reactions related to splenocytes activities were found in all treated groups of mice. Despite its promising results, the concentration of both <jats:italic>C. nutans<\/jats:italic> extracts have also reduced the number of colonies formed in the liver and lungs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In conclusion, <jats:italic>C. nutans<\/jats:italic> extracts exert antitumor and antioxidant activities against 4\u2009T1 mouse breast model with no adverse effect and inflammatory response at high dose of 1000\u2009mg\/kg, indicating an effective and complementary approach for cancer prevention and treatment.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12906-019-2757-4","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T17:02:44Z","timestamp":1575046964000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Antitumor and antioxidant effects of Clinacanthus nutans Lindau in 4\u2009T1 tumor-bearing mice"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4835-4687","authenticated-orcid":false,"given":"Nik Mohd Afizan","family":"Nik Abd Rahman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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