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Recent studies have revealed a high incidence of drug-resistant <jats:italic>Candida<\/jats:italic> strains. Additionally, treating Candida vulvovaginitis during pregnancy is challenging as antifungal therapy is associated with fetal abnormalities. Hence, it is important to develop novel therapeutic strategies to treat vulvovaginal candidiasis.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>In this study, we used the disc diffusion method to evaluate the anticandidal activity of different <jats:italic>Syzygium aromaticum<\/jats:italic> extracts (methanol, ethyl acetate, n-hexane, and diethyl ether) against <jats:italic>C. albicans<\/jats:italic>, <jats:italic>C. glabrata,<\/jats:italic> and <jats:italic>C. tropicalis<\/jats:italic>. Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis of different <jats:italic>S. aromaticum<\/jats:italic> extracts was performed to determine active components exhibiting anticandidal activity. Cytotoxicity of different clove extracts against the HUH7 cell line was evaluated.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The ethyl acetate extract exhibited the highest antifungal activity against <jats:italic>C. albicans<\/jats:italic>, <jats:italic>C. glabrata<\/jats:italic>, and <jats:italic>C. tropicalis<\/jats:italic> with inhibition zone diameters of 20.9, 14.9, and 30.7\u2009mm, respectively. The minimum inhibitory concentration of the <jats:italic>S. aromaticum<\/jats:italic> ethyl acetate extract was 250\u2009\u03bcg\/disc against <jats:italic>C. tropicalis<\/jats:italic>, and 500\u2009\u03bcg\/disc against <jats:italic>C. albicans<\/jats:italic> and <jats:italic>C. glabrata<\/jats:italic>, while the minimum fungicidal concentration was 0.5\u2009mg\/disc against <jats:italic>C. tropicalis<\/jats:italic> and 1\u2009mg\/disc against the <jats:italic>C. albicans<\/jats:italic> and <jats:italic>C. glabrata<\/jats:italic>. GC-MS analysis of the ethyl acetate extract revealed the main bioactive compound as eugenol (58.88%), followed by eugenyl acetate (23.86%), trans-caryophyllene (14.44%), and \u03b1-humulene (1.88%). The cytotoxicity assay indicated that the diethyl ether extract demonstrated the lowest toxicological effect against the HUH7 cell line, with a relative IC<jats:sub>50<\/jats:sub> of 62.43\u2009\u03bcg\/ml; the methanolic extract demonstrated a higher toxicity (IC<jats:sub>50<\/jats:sub>, 24.17\u2009\u03bcg\/ml).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>As the <jats:italic>S. aromaticum<\/jats:italic> extract exhibited high antifungal activity at low concentrations, it can be a potential source of natural antifungal drugs.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12906-020-2818-8","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T17:04:28Z","timestamp":1580403868000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0997-8350","authenticated-orcid":false,"given":"Mohamed Taha","family":"Yassin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf Abdel-Fattah","family":"Mostafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz Abdulrahman","family":"Al-Askar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,30]]},"reference":[{"issue":"6","key":"2818_CR1","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1093\/mmy\/myy117","volume":"57","author":"Mohammed A Farhan","year":"2018","unstructured":"Farhan MA, Moharram AM, Salah T, Shaaban OM. 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