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This was accomplished by using biochemical and cellular assays, including MTT, caspase Glo, western blot, and annexin V\/propidium iodide-based flow cytometry analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Test of the dichloromethane fraction of the methanolic extract of<jats:italic>C. droserifolia,<\/jats:italic>(CDD) revealed potent cytotoxic activity (from 70 to 90%) against several human cancer cell lines, including MCF-7, MDA-MB-231, and HeLa. Further characterization of the CDD fraction in MCF-7 cells revealed that it could activate the enzymatic activity of various caspases in a statistically significant manner, and induce cleavage of both caspase 7 and poly ADB ribose polymerase (PARP) proteins, but not the ethyl acetate fraction. Test of the ability of CDD to induce early signs of apoptosis was validated by annexin V\/propidium iodide assay using FACS analysis. Induction of apoptosis was completely reversed by the classic pan inhibitor of apoptosis, Z-VAD-FMK, reducing early apoptosis from 29.7 to 0.6%, confirming that CDD could induce caspase-dependent apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Altogether, our results reveal that<jats:italic>C. droserifolia<\/jats:italic>is a valuable medicinal plant with bioactive molecules that can induce apoptosis in human cancer cells. 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