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Benznidazole (BZ), a drug available for its treatment, has limited efficacy and significant side effects. Essential oils (EOs) have demonstrated trypanocidal activity and may constitute a therapeutic alternative. Our aim was to evaluate the efficacy of the EOs of clove (CEO -\n                      <jats:italic>Syzygium aromaticum<\/jats:italic>\n                      ) and ginger (GEO -\n                      <jats:italic>Zingiber officinale<\/jats:italic>\n                      ), administered alone and in combination with BZ, in Swiss mice infected with\n                      <jats:italic>T. cruzi.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The animals were inoculated with 10,000 blood trypomastigotes of the Y strain of\n                      <jats:italic>T. cruzi<\/jats:italic>\n                      II by gavage and divided into four groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912 to 15): 1) untreated control (NT); 2) treated with BZ; 3) treated with CEO or GEO; and 4) treated with BZ\u2009+\u2009CEO or GEO. The treatments consisted of oral administration of 100\u2009mg\/kg\/day, from the 5th day after parasite inoculation, for 20 consecutive days. All groups were submitted to fresh blood examination (FBE), blood culture (BC), conventional PCR (cPCR) and real-time PCR (qPCR), before and after immunosuppression with cyclophosphamide.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Clove and ginger EOs, administered alone and in combination with BZ, promoted suppression of parasitemia (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), except for the animals treated with CEO alone, which presented a parasitemia curve similar to NT animals. However, there was a decrease in the BC positivity rate (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and parasite load (&lt;\u20090.0001) in this group. Treatment with GEO alone, on the other hand, besides promoting a decrease in the BC positivity rate (p\u2009&lt;\u20090.05) and parasite load (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), this EO also resulted in a decrease in mortality rate (p\u2009&lt;\u20090.05) of treated mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Decreased parasite load, as detected by qPCR, was observed in all treatment groups (BZ, CEO, GEO and BZ\u2009+\u2009EOs), demonstrating benefits even in the absence of parasitological cure, thus opening perspectives for further studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12906-021-03248-8","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T08:03:29Z","timestamp":1614240209000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II"],"prefix":"10.1186","volume":"21","author":[{"given":"Marcella Paula Mansano","family":"Sarto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hevillyn Fernanda","family":"Lucas da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilma","family":"de Souza Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"de Abreu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson","family":"Zanusso Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6314-8668","authenticated-orcid":false,"given":"Max Jean","family":"de Ornelas Toledo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"issue":"2","key":"3248_CR1","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","volume":"1","author":"C Chagas","year":"1909","unstructured":"Chagas C. 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