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Natural products are considered as the antioxidants enrich food that can restrict the progress of oxidative stress induced disorders. Therefore, the present study was aimed to evaluate the renal protective effect of <jats:italic>Ajuga parviflora<\/jats:italic> leaf extract in carbon tetrachloride intoxicated rats.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The hydromethanolic extract of <jats:italic>A. parviflora<\/jats:italic> leaves was obtained by extracting twice in 60% methanol. The principal bioactive constituents were detected by LC\/MS analysis. Toxicity of plant extract was assessed using brine shrimp lethal toxicity test and acute toxicity model on healthy Sprague-Dawley male rats. Nephroprotective effects of plant extract were also evaluated on rats by inducing CCl<jats:sub>4<\/jats:sub> renal toxicity in comparison with positive control and na\u00efve groups. The dose of <jats:italic>A. parviflora<\/jats:italic> administered to animal was 100, 200 and 300\u2009mg\/kg. All administrations were given orally on an alternate day basis for 30\u2009days. Urine and serum biomarkers were analyzed, along with antioxidant enzymes. Finally, the DNA damages, lipid peroxides, hydrogen peroxides and nitrites were assessed in rat\u2019s renal tissue. The histopathology alterations in renal tissues were further studied for kidney damages.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The LC\/MS analysis confirmed the presence of different important pharmacological compounds in <jats:italic>A. parviflora<\/jats:italic> methanolic leaf extract. The key bioactive compounds include pyocyanin, zonisamide, D Saccharic acid, altretamine, carbocyclic thromboxane A2, Sinapyl alcohol, and vitamin C. The important polypeptides identified include Lys-Tyr-Lys, His-His-Lys, Met-Asp-Arg, Phe-Val-Arg, and PyroGlu-Val-Arg. The LD<jats:sub>50<\/jats:sub> of <jats:italic>A. parviflora<\/jats:italic> was found to be &gt;\u20091000\u2009\u03bcg\/mL. <jats:italic>A. parviflora<\/jats:italic> administration significantly subsides CCl<jats:sub>4<\/jats:sub> toxicity in rats, reduced the elevated level of RBCs, pus and epithelial cells. The abnormal elevated level of specific gravity, creatinine, urobilinogen, urea and albumin were also reduced to normal physiological level. The reduced urinary protein and pH were also normalized. The serum urobilinogen, urea and total bilirubin levels were also reversed to normal levels while the diminished albumin and total protein levels also came to normal. The important phase I and II enzyme levels were also reversed in <jats:italic>A. parviflora<\/jats:italic> administered rats. The H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, thiobarbituric acid reactive substance (TBARS) and nitrite levels were significantly decreased. Furthermore, the damaged DNA and histopathological changes in CCl<jats:sub>4<\/jats:sub> exposed rats were also highly significantly reversed after the administration of <jats:italic>A. parviflora<\/jats:italic>. All effects were significant (<jats:italic>P\u2009&lt;\u20090.05<\/jats:italic>) and highly significant (<jats:italic>P\u2009&lt;\u20090.005<\/jats:italic>) at 100 and 300\u2009mg\/kg respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The restored urine and serum profile of various parameters to normal physiological levels suggests that the <jats:italic>A. parviflora<\/jats:italic> has potential antioxidant and repairing potential in renal disorders.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12906-021-03360-9","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T13:03:19Z","timestamp":1626094999000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Phytochemical, acute toxicity and renal protective appraisal of Ajuga parviflora hydromethanolic leaf extract against CCl4 induced renal injury in rats"],"prefix":"10.1186","volume":"21","author":[{"given":"Samiullah","family":"Burki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeba Gul","family":"Burki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Arif","family":"Asghar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imdad","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saba","family":"Zafar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,12]]},"reference":[{"issue":"2","key":"3360_CR1","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1081\/JDI-120018723","volume":"25","author":"J Prakash","year":"2003","unstructured":"Prakash J, Sen D, Sarat Kumar N, Kumar H, Tripathi L, Saxena R. 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