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The major target organ of PQ poisoning is the lungs. The pathogenesis of PQ-induced lung injury involves oxidative stress and inflammation. Unfortunately, there is still no effective antidote for PQ poisoning. We hypothesized that aqueous\n                      <jats:italic>Thunbergia laurifolia<\/jats:italic>\n                      (TL) leaf extract is a possible antidote for PQ-induced lung injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The total phenolic content and caffeic acid content of an aqueous extract of TL leaves were analyzed. Male Wistar rats were randomly divided into four groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094 per group): the control group (administered normal saline), the PQ group (administered 18\u2009mg\/kg body weight (BW) PQ dichloride subcutaneously), the PQ\u2009+\u2009TL-low-dose (LD) group (administered PQ dichloride subcutaneously and 100\u2009mg\/kg BW aqueous TL leaf extract by oral gavage) and the PQ\u2009+\u2009TL-high-dose (HD) group (administered PQ dichloride subcutaneously and 200\u2009mg\/kg BW aqueous TL leaf extract by oral gavage). Malondialdehyde (MDA) levels and lung histopathology were analyzed. In addition, the mRNA expression of NADPH oxidase (NOX), interleukin 1 beta (IL-1\u03b2), and tumor necrosis factor alpha (TNF-\u03b1) was assessed using reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression of IL-1\u03b2 and TNF-\u03b1 was analyzed using immunohistochemistry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The total phenolic content of the extract was 20.1\u2009\u00b1\u20090.39\u2009\u03bcg gallic acid equivalents (Eq)\/mg extract, and the caffeic acid content was 0.31\u2009\u00b1\u20090.01\u2009\u03bcg\/mg. The PQ group showed significantly higher MDA levels and NOX, IL-1\u03b2 and TNF-\u03b1 mRNA expression than the control group. Significant pathological changes, including alveolar edema, diffuse alveolar collapse, hemorrhage, leukocyte infiltration, alveolar septal thickening and vascular congestion, were observed in the PQ group compared with the control group. However, the aqueous TL leaf extract significantly attenuated the PQ-induced increases in MDA levels and NOX, IL-1\u03b2 and TNF-\u03b1 expressions. Moreover, the aqueous TL leaf extract ameliorated PQ-induced lung pathology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study indicates that aqueous TL leaf extract can ameliorate PQ-induced lung pathology by modulating oxidative stress through inhibition of NOX and by regulating inflammation through inhibition of IL-1\u03b2 and TNF-\u03b1 expressions. We suggest that aqueous TL leaf extract can be used as an antidote for PQ-induced lung injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12906-022-03567-4","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T08:02:48Z","timestamp":1647936168000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Aqueous Thunbergia laurifolia leaf extract alleviates paraquat-induced lung injury in rats by inhibiting oxidative stress and inflammation"],"prefix":"10.1186","volume":"22","author":[{"given":"Sarawoot","family":"Palipoch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuchard","family":"Punsawad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phanit","family":"Koomhin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasit","family":"Na-Ek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasinee","family":"Poonsawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungruedi","family":"Kimseng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Potiga","family":"Chotipong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kingkan","family":"Bunluepuech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorawit","family":"Yusakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasit","family":"Suwannalert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,22]]},"reference":[{"issue":"15","key":"3567_CR1","doi-asserted-by":"publisher","first-page":"e9621","DOI":"10.1097\/MD.0000000000009621","volume":"97","author":"N Elenga","year":"2018","unstructured":"Elenga N, Merlin C, Le Guern R, Kom-Tchameni R, Ducrot YM, Pradier M, et al. 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