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Besides, there was no quantitative evaluation done for phytochemicals previously. Therefore, the main purpose of this research work is to evaluate the antimicrobial activity and quantitative evaluation of the phenol and flavonoid contents of<jats:italic>V. auriculifera<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of this study was to conduct the antimicrobial and quantitative evaluation of a sequentially extracted leaf of<jats:italic>Vernonia auriculifera Hiern<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The leaves of the plant were cleaned with tap water and air-dried. The grounded leaf product was subsequently extracted by hexane, chloroform, and methanol in maceration flasks. Then the total phenol and total flavonoid content in each extract were determined. Standard strains of bacterial and fungal species were used to assess the antibacterial, and antifungal susceptibility test and to determine the minimum inhibitory concentration of crude extract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Extraction yield for hexane, chloroform, and methanol was 0.6\u2009\u00b1\u20090.05%, 1.7\u2009\u00b1\u20090.02%, and 3.3\u2009\u00b1\u20090.01% respectively. The total phenolic content of methanol extract was 72.998\u2009\u00b1\u20090.002\u00a0mg GAE \/g. The total flavonoid content of hexane and chloroform extracts were 2.59\u2009\u00b1\u20090.004\u00a0mg QE\/g and 9.6\u2009\u00b1\u20090.02\u00a0mg QE\/g respectively. The antimicrobial activity test showed the chloroform extract was the most active against all test microorganisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study has shown the activity of<jats:italic>V. auriculifera<\/jats:italic>against selected microorganisms of study. The chloroform extract was the most active as compared to the hexane and methanol extracts.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12906-022-03690-2","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T09:04:58Z","timestamp":1660295098000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Antimicrobial Evaluation of Sequentially extracted Leaf of Vernonia auriculifera Hiern (Rejicho)"],"prefix":"10.1186","volume":"22","author":[{"given":"Teshale Etiso","family":"Wado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultan","family":"Suleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tesfaye","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"3690_CR1","first-page":"64","volume-title":"Medicinal plants and traditional medicine in Africa","author":"EA Sofowora","year":"1982","unstructured":"Sofowora EA. 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Afr Health Sci. 2016;16(2):629\u201339.","journal-title":"Afr Health Sci"}],"container-title":["BMC Complementary Medicine and Therapies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12906-022-03690-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12906-022-03690-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12906-022-03690-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,25]],"date-time":"2023-11-25T15:09:48Z","timestamp":1700924988000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmccomplementalternmed.biomedcentral.com\/articles\/10.1186\/s12906-022-03690-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,12]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["3690"],"URL":"https:\/\/doi.org\/10.1186\/s12906-022-03690-2","relation":{},"ISSN":["2662-7671"],"issn-type":[{"value":"2662-7671","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,12]]},"assertion":[{"value":"13 May 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 July 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 August 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The <i>Vernonia auriculifera<\/i> Hiern plant identification was performed at the National Herbarium of Addis Ababa University by Melaku Wendafrash, senior botanist, and a voucher specimen was deposited with the TE001 voucher number.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All authors declared that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"219"}}